A5002352885- Drug-Induced Hepatotoxicity and Protection
- Protease and Inhibitor Mechanisms
- Food Allergy and Anaphylaxis Research
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Asthma and respiratory diseases
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Wound Healing and Treatments
- IL-33, ST2, and ILC Pathways
- Allergic Rhinitis and Sensitization
- Lymphatic System and Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Rheumatoid Arthritis Research and Therapies
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- Drug Transport and Resistance Mechanisms
- Dental materials and restorations
- Immune Response and Inflammation
- Endodontics and Root Canal Treatments
- Research on Leishmaniasis Studies
- Antimicrobial agents and applications
- Hippo pathway signaling and YAP/TAZ
- Liver physiology and pathology
- Celiac Disease Research and Management
Rega Institute for Medical Research
2020-2024
KU Leuven
2020-2024
Hospital for Sick Children
2018-2023
Universidade Federal de Minas Gerais
2013-2019
Southwest Bahia State University
2019
Universidade Federal de Santa Catarina
2012-2017
Pontifical Catholic University of Rio de Janeiro
2004
Drug‐induced liver injury (DILI) is an important cause of acute failure, with limited therapeutic options. During DILI, oncotic necrosis concomitant release and recognition intracellular content amplifies inflammation injury. Among these molecules, self‐DNA has been widely shown to trigger inflammatory autoimmune diseases; however, whether DNA released from damaged hepatocytes accumulates into necrotic the impact its by immune system remains elusive. Here we show that treatment two different...
Introduction Wound healing is a complex process to restore homeostasis after injury and insufficient skin wound considerable problem in medicine. Whereas many attempts of regenerative medicine have been made for with growth factors cell therapies, simple pharmacological immunological studies are lagging behind. We investigated how fibrin hydrogels modulate immune cells molecules mice. Methods Physiological (3.5 mg/mL fibrinogen) were generated, biophysically analyzed stiffness protein...
Abstract Background Adenosine triphosphate (ATP) is secreted from hepatocytes under physiological conditions and plays an important role in liver biology through the activation of P2 receptors. Conversely, higher extracellular ATP concentrations, as observed during necrosis, trigger inflammatory responses that contribute to progression injury. Impaired calcium (Ca 2+ ) homeostasis a hallmark acetaminophen (APAP)-induced hepatotoxicity, since induces mobilization intracellular Ca stocks, we...
Acetaminophen (APAP) is usually safe when administrated in therapeutic doses; however, APAP overdose can lead to severe liver injury. cause direct hepatocyte damage, and stimulates an inflammatory response leading oxidative stress. Supressor of Cytokine Signaling (SOCS) 2 modulates cytokine growth factor signaling, plays a role the regulation hepatic cellular processes. Our study evaluated SOCS2 The administration toxic dose (600 mg/kg) caused significant necrosis WT mice. In SOCS2-/- mice,...
This work reports the development of a new, low-friction coefficient combined with high mechanical strength and scuffing resistance self-lubricating composite produced by powder injection moulding process recently introduced plasma-assisted debinding sintering process. We present discuss effect temperature SiC content on microstructure, friction properties. Three different temperatures (1100,1150, 1200°C) six contents (0–5%) were analysed. had paramount role behaviour. Sintering almost no...
Whereas the blood microvasculature constitutes a biological barrier to action of blood-borne insulin on target tissues, lymphatic might act as subcutaneously administrated reaching circulation. Here, we evaluate interaction with primary microvascular endothelial cells [human dermal (HDLEC)] and adipose (HAMEC)] origin, derived from human respectively. HDLEC express higher levels receptor signal in response low 2.5 nM, while HAMEC only activate signaling at 100 nM (a dose that vessels do not...
In the present study we evaluated protection raised by immunization with recombinant influenza viruses carrying sequences coding for polypeptides corresponding to medial and carboxi-terminal moieties of Trypanosoma cruzi ´s amastigote surface protein 2 (ASP2). Those were used in sequential adenovirus (heterologous prime-boost protocol) encoding complete sequence ASP2 (Ad-ASP2) two mouse strains (C57BL/6 C3H/He). The CD8 effector response elicited this protocol was comparable that observed...
Acetaminophen (APAP) poisoning is one of the leading causes acute hepatic failure and liver transplantation often only lifesaving alternative. During course hepatocyte necrosis, an intense accumulation neutrophils observed within microenvironment. Despite classic idea that neutrophil in tissues collateral tissue damage, there a growing body evidence showing can also orchestrate resolution inflammation. In this work, drug-induced injury was induced by oral administration APAP pharmacological...
To propose an alternative model of hepatic encephalopathy (HE) in mice, resembling the human features disease.Mice received two consecutive intraperitoneal injections thioacetamide (TAA) at low dosage (300 mg/kg). Liver injury was assessed by serum transaminase levels (ALT) and liver histology (hematoxylin eosin). Neutrophil infiltration estimated confocal intravital microscopy. Coagulopathy evaluated using prolonged prothrombin partial thromboplastin time. Hemodynamic parameters were...
A fundamental part of the immune response to infection or injury is leukocyte migration. Matrix metalloproteinases (MMPs) are a class secreted cell-bound endopeptidases, implicated in every step process inflammatory cell Hence, specific inhibition MMPs an interesting approach control inflammation. We evaluated potential bivalent carboxylate inhibitor selectively inhibit trimeric proteoform MMP-9 and compared this with corresponding monovalent inhibitor. The efficiently inhibited (IC50 = 0.1...
The lymphatic circulation regulates transfer of tissue fluid and immune cells toward the venous circulation. While obesity impairs vessel function, contribution endothelial (LEC) to metabolic disease phenotypes is poorly understood. LEC microvessels are in direct contact with interstitial fluid, whose composition changes during development obesity, markedly by increases saturated fatty acids. Palmitate, most prevalent acid lymph blood, detrimental metabolism function diverse tissues, but its...
Drug-induced liver injury (DILI) is a major cause of acute failure (ALF), where hepatocyte necrotic products trigger inflammation, release CXC chemokine receptor 2 (CXCR2) ligands (IL-8) and other neutrophil chemotactic molecules. Liver infiltration by neutrophils the life-threatening tissue damage that ensues. A GRPR (gastrin-releasing peptide receptor) antagonist impairs IL-8-induced chemotaxis in vitro. We investigated its potential to reduce acetaminophen-induced ALF, migration,...
The prevalence of food allergy is rising in the western world. Allergen restriction chosen treatment this condition, but continuous ingestion antigen has shown positive results clinical trials. In a previous study, we have several allergic and metabolic alterations after 7 days ovalbumin (OVA) by sensitized mice. aim study was to investigate whether prolonged mice would reverse consequences caused experimental allergy. For this, parameters were analysed an OVA diet OVA-sensitized As...
SDF-1/CXCL12 is a unique chemotactic factor with multiple functions on various types of precursor cells, all carrying the cognate receptor CXCR4. Whereas individual biological have been well documented, practical applications in medicine are insufficiently studied. This explained by complex multifunctional biology SDF-1 systemic and local effects, critical dependence activity aminoterminal proteolytic processing limited knowledge applicable modulators its activity. We here present new...
The rise of human life expectancy results in the increasing elderly population and consequently diseases old age, which are mostly related with bone degenerative diseases. These problems also affect young individuals, commonly due to accidents (automobile work). This fact has stimulated research development materials that can replace or regenerate damaged bone. From engineering view, is a composite material consisting an organic matrix (collagen), reinforced by inorganic component...
Abstract Tailored hydrogels mimicking the native extracellular environment could help overcome high variability in outcomes within regenerative endodontics. This study aimed to evaluate effect of chemokine-binding and antimicrobial polymer, chlorite-oxidized oxyamylose (COAM), on microstructural properties fibrin self-assembling peptide (SAP) hydrogels. A further goal was assess influence differences between vitro behavior human dental pulp stem cells (hDPSCs). Structural mechanical...
Objectives To explore posttranslational modifications (PTMs), including proteolytic activation, multimerization, complex formation and citrullination of gelatinases, in particular gelatinase B/MMP-9, to detect gelatin-Sepharose affinity-purified synovial fluids, the presence specific MMP proteoforms relation arthritis. Methods Latent, activated, complexed truncated gelatinase-A/MMP-2 B/MMP-9 were detected with use zymography analysis compare levels, substrate conversion assays, test net...
Concomitant chronic diseases are a common finding in clinics and may consist major issue therapeutics. Here, we investigated whether prolonged ingestion of ovalbumin (Ova) by sensitized mice would reduce the severity an associated concurrent immunomediated condition such as antigen-induced arthritis (AIA). AIA was induced administration methylated bovine albumin (mBSA) into knee joints previously immunized mice, evaluated articular leukocyte trafficking levels cytokines (TNF-α, IL-1β)...