A5065451968- Respiratory viral infections research
- Neonatal Respiratory Health Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Hepatitis B Virus Studies
- Antimicrobial Peptides and Activities
- Influenza Virus Research Studies
- Immune cells in cancer
- Bacteriophages and microbial interactions
- Pediatric health and respiratory diseases
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Phagocytosis and Immune Regulation
- Immunotherapy and Immune Responses
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neuropeptides and Animal Physiology
- Cancer, Stress, Anesthesia, and Immune Response
- Bipolar Disorder and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Marine Invertebrate Physiology and Ecology
University of Manitoba
2021-2023
Research Manitoba
2022-2023
Children's Hospital Research Institute of Manitoba
2022-2023
Pontifícia Universidade Católica do Rio Grande do Sul
2010-2021
Hospital for Sick Children
2019-2021
Universidade Federal do Rio de Janeiro
2007-2011
Universidade Federal de Lavras
2010
Heme is an ancient and ubiquitous molecule present in organisms of all kingdoms, composed atom iron linked to four ligand groups porphyrin. A high amount free heme, a potential amplifier the inflammatory response, characteristic feature diseases with increased hemolysis or extensive cell damage. Here we demonstrate that but not its analogs/precursors, induced tumor necrosis factor-alpha (TNF-alpha) secretion by macrophages dependently on MyD88, TLR4, CD14. The activation TLR4 heme...
Acute viral bronchiolitis by Respiratory Syncytial Virus (RSV) is the most common respiratory illness in children first year of life. RSV generates large numbers hospitalizations and an important burden to health systems. Neutrophils their products are present airways RSV-infected patients who developed increased lung disease. Neutrophil Extracellular Traps (NETs) formed release granular nuclear contents neutrophils extracellular space response different stimuli recent studies have proposed...
Hemolysis or extensive cell damage can lead to high concentrations of free heme, causing oxidative stress and inflammation. Considering that heme induces neutrophil chemotaxis, we hypothesize activates a G protein-coupled receptor. Here show similar several analogs were able induce migration in vitro vivo. Mesoporphyrins, molecules lacking the vinyl groups their rings, not chemotactic for neutrophils selectively inhibited heme-induced migration. Moreover, induced by was abolished...
Abstract Respiratory syncytial virus (RSV) is a major cause of diseases the respiratory tract in young children and babies, being mainly associated with bronchiolitis. RSV infection occurs primarily pulmonary epithelial cells and, once established, an immune response triggered neutrophils are recruited. In this study, we investigated mechanisms underlying NET production induced by RSV. We show that classical ROS-dependent NETosis human was trapped DNA lattices coated NE MPO. induction...
Neutrophil extracellular traps (NETs) are a combination of DNA fibers and granular proteins, such as neutrophil elastase (NE). NETs released in the space response to different stimuli. Carrageenan is sulfated polysaccharide extracted from Chondrus crispus , marine algae, used for decades research its potential induce inflammation animal models. In this study, we show first time that carrageenan injection can NET release mouse model acute peritonitis. induced by viable neutrophils with NE...
Respiratory syncytial virus (RSV) is the major cause of acute bronchiolitis in infants under 2 years old. Necroptosis has been implicated outcomes respiratory infections. We report that RSV infection triggers necroptosis primary mouse macrophages and human monocytes a RIPK1-, RIPK3- MLKL-dependent manner. Moreover, pathways are harmful to clearance from alveolar macrophages. Additionally, Ripk3 −/− mice were protected RSV-induced weight loss presented with reduced viral loads lungs. Alveolar...
Neutrophil migration to inflamed sites is crucial for both the initiation of inflammation and resolution infection, yet these cells are involved in perpetuation different chronic inflammatory diseases. Gastrin-releasing peptide (GRP) a neuropeptide that acts through G protein coupled receptors (GPCRs) signal transmission central peripheral nervous systems. Its receptor, gastrin-releasing receptor (GRPR), expressed by various cell types, it overexpressed cancer cells. RC-3095 selective GRPR...
High concentrations of free heme found during hemolytic events or cell damage leads to inflammation, characterized by neutrophil recruitment and production reactive oxygen species, through mechanisms not yet elucidated. In this study, we provide evidence that heme-induced neutrophilic inflammation depends on endogenous activity the macrophage-derived lipid mediator leukotriene B(4) (LTB(4)). vivo, into peritoneal cavity mice was attenuated pretreatment with 5-lipoxygenase (5-LO) inhibitors...
Trypanosoma cruzi, the etiological agent of Chagas disease, is transmitted by bug feces deposited on human skin during a blood meal. However, parasite infection occurs through wound produced insect mouthparts. Saliva Triatominae Rhodnius prolixus source lysophosphatidylcholine (LPC). Here, we tested role both triatomine saliva and LPC transmission. We show that vector powerful inducer cell chemotaxis. A massive number inflammatory cells were found at sites where or was inoculated into mice....
Purpose: Asthma is a highly prevalent chronic inflammatory lung disease characterized by airway hyperresponsiveness to allergens, edema, and increased mucus secretion. Such can be liquefied recombinant human deoxyribonuclease (rhDNase), in which efficacy of rhDNase has been well documented patients with cystic fibrosis, but little studied asthma. In the present study, we investigated whether intranasal administration improved inflammation pulmonary function an experimental model Methods:...
Background Heat shock proteins (Hsps) are stress induced with immunomodulatory properties. The Hsp70 of Mycobacterium tuberculosis (TBHsp70) has been shown to have an anti-inflammatory role on rodent autoimmune arthritis models, and the protective effects were demonstrated be dependent interleukin-10 (IL-10). We previously observed that TBHsp70 inhibited maturation dendritic cells (DCs) IL-10 production by these cells, as well in synovial fluid cells. Methodology/Principal Findings...
Asthma is associated with a loss of the structural integrity airway epithelium and dysfunction physical barrier, which protects airways from external harmful factors. Granulocyte activation causes formation extracellular traps, releasing web-like structures DNA proteins, being important to kill pathogens extracellularly. We investigated whether eosinophils infiltrating in an experimental model asthma would induce eosinophil traps (EETs) bronchoalveolar lavage fluid lung tissue. showed that...
Gastric infection by Helicobacter pylori is considered a risk factor for gastric and duodenal cancer, extragastric diseases. Previous data have shown that, in non-enzymatic way, H. urease (HPU) activates neutrophils to produce ROS also induces platelet aggregation, requiring ADP secretion modulated the 12-lipoxygenase pathway, signaling cascade triggered physiological agonist collagen. Here we investigated further effects on platelets of recombinant versions holoenzyme HPU, its two subunits...
Background. Respiratory syncytial virus (RSV) infection is the major cause of respiratory disease in lower tract infants and young children. Attempts to develop effective vaccines or pharmacological treatments inhibit RSV without undesired effects on human health have been unsuccessful. However, has reported be affected by flavonoids. The mechanisms underlying viral inhibition induced these compounds are largely unknown, making development new drugs difficult. Methods. To understand...
Drug-induced liver injury (DILI) is a major cause of acute failure (ALF), where hepatocyte necrotic products trigger inflammation, release CXC chemokine receptor 2 (CXCR2) ligands (IL-8) and other neutrophil chemotactic molecules. Liver infiltration by neutrophils the life-threatening tissue damage that ensues. A GRPR (gastrin-releasing peptide receptor) antagonist impairs IL-8-induced chemotaxis in vitro. We investigated its potential to reduce acetaminophen-induced ALF, migration,...