A5068816139- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Drug Transport and Resistance Mechanisms
- Synthesis and Biological Evaluation
- Pneumocystis jirovecii pneumonia detection and treatment
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Cancer therapeutics and mechanisms
- Ion Transport and Channel Regulation
- Oral microbiology and periodontitis research
- HIV/AIDS drug development and treatment
- Antibiotic Resistance in Bacteria
- Plant-Derived Bioactive Compounds
- Trace Elements in Health
- Microbial Natural Products and Biosynthesis
- Natural product bioactivities and synthesis
- ATP Synthase and ATPases Research
- Ion channel regulation and function
- Marine Sponges and Natural Products
- Phytochemistry and Bioactivity Studies
- Antimicrobial agents and applications
- Biochemical and Molecular Research
- Cholinesterase and Neurodegenerative Diseases
- Piperaceae Chemical and Biological Studies
- Enzyme function and inhibition
Universidade Federal do Rio de Janeiro
2015-2024
University of Beira Interior
2019
Czech Academy of Sciences, Institute of Microbiology
2018
Universidade do Estado do Rio de Janeiro
2002
The novel coronavirus SARS-CoV-2 causes COVID-19, a highly pathogenic viral infection threatening millions. majority of the individuals infected are asymptomatic or mildly symptomatic showing typical clinical signs common cold. However, approximately 20% patients can progress to acute respiratory distress syndrome (ARDS), evolving death in about 5% cases. Recently, angiotensin-converting enzyme 2 (ACE2) has been shown be functional receptor for virus entry into host target cells....
Background The parasitic trematode Schistosoma mansoni is one of the major causative agents human schistosomiasis, which afflicts 200 million people worldwide. Praziquantel remains main drug used for schistosomiasis treatment, and reliance on single therapy has been prompting search new therapeutic compounds against this disease. Our group demonstrated that heme crystallization into hemozoin (Hz) within S. gut a detoxification route with lipid droplets involved in process acting as potential...
Abstract Re‐emergence of vector‐borne diseases such as dengue and yellow fever, which are both transmitted by the A edes aegypti mosquito, has been correlated with insecticide resistance. P ‐glycoproteins ( ‐gps) ATP ‐dependent efflux pumps that involved in transport substrates across membranes. Some these proteins have implicated multidrug resistance MDR ). In this study, we identified a putative ‐glycoprotein Ae . database based on its significantly high identity nopheles gambiae , C ulex...
Trypanosoma cruzi, the etiological agent of Chagas disease, is transmitted by bug feces deposited on human skin during a blood meal. However, parasite infection occurs through wound produced insect mouthparts. Saliva Triatominae Rhodnius prolixus source lysophosphatidylcholine (LPC). Here, we tested role both triatomine saliva and LPC transmission. We show that vector powerful inducer cell chemotaxis. A massive number inflammatory cells were found at sites where or was inoculated into mice....
The primary objective of this work was to evaluate the capability curcumin, a natural compound found in Curcuma longa plant, sensitize clinical isolate Candida albicans, which have high resistance fluconazole. In addition, we assessed whether result existence efflux pumps, could confer multiple drug phenotype. To azole resistance, used Clinical Laboratory Standard Institute (CLSI) MIC assays procedures with minor modifications. For evaluation synergistic interaction curcumin and fluconazole,...
Candidaemia is a public health problem mainly in hospitalized individuals worldwide. In Brazil, Candida albicans the most prevalent species that causes candidaemia, followed by tropicalis and parapsilosis . Few data on abundance of antifungal resistance are available for Latin America.We analysed frequency azole echinocandin isolates ( n = 75) collected between 2012 2014 at University Hospital Federal Juiz de Fora (Brazil). The primary targets erg11 (azoles) fks1 (echinocandins) were...
Baker's yeast cells accumulate osmolytes as a response to several stress conditions such hightemperature and low‐temperature shifts, dehydration, or osmotic stress. One of the major that accumulates is trehalose, which plays an essential role affecting survival at time In this report, we show trehalose efficiently protects function structure two cytosolic enzymes against chemical denaturation by guanidinium chloride. Other sugars tested also protected pyrophosphatase glucose‐6‐phosphate...
Adult Schistosoma mansoni digest large amounts of host hemoglobin and release potentially toxic heme inside their guts. We have previously demonstrated that free in S. is detoxified through aggregation, forming hemozoin (Hz). Possible mechanisms aggregation the effects chloroquine (CLQ) on formation Hz viability this parasite now been investigated. Different fractions isolated from mansoni, such as crude whole-worm homogenates, total lipid extracts, itself promoted vitro a CLQ-sensitive...
The ABCG2 multidrug transporter is known to confer cancer cell resistance by causing the efflux of anticancer drugs; therefore, selective inhibitors have potential improve chemotherapeutic treatments. Here, various methoxy derivatives resveratrol are shown be potent mitoxantrone ABCG2: among a series 11 derivatives, compound 9 (3,5,3',4'-tetramethoxy trans-stilbene) had an IC(50) 0.16 μM and showed maximal inhibition 75%, as measured flow cytometry. It was not transported, HPLC fractionation...
Cardiotonic steroids are used to treat heart failure and arrhythmia have promising anticancer effects. The prototypic cardiotonic steroid ouabain may also be a hormone that modulates epithelial cell adhesion. consist of nucleus lactone ring, their biological effects depend on the binding receptor, Na,K-ATPase, through which, they inhibit Na+ K+ ion transport activate several intracellular signaling pathways. In this study, we added styrene group ring digoxin, obtain 21-benzylidene digoxin...
Casearia genus (Salicaceae) is found in sub-tropical and tropical regions of the world comprises about 160-200 species. It a medicinal plant used South America, also known as "guaçatonga", "erva-de-tiú", "cafezinho-do-mato". In Brazil, there are 48 species 12 registered State Rio de Janeiro, including sylvestris Sw. There many studies related to chemical profile cytotoxic activities extracts from these plants, although few antifungal potential essential oil have been reported. this work, we...
The increase in the prevalence and severity of fungal infections resistance to available antifungals highlights imperative need for novel therapeutics search new targets. High-content screening libraries containing hundreds compounds is a powerful strategy searching drug candidates. In this study, we screened Pandemic Response Box library (Medicines Malaria Venture) 400 diverse molecules against Sporothrix pathogenic species. initial screen identified twenty-four candidates that inhibited...
Pdr5p, the major multidrug exporter in Saccharomyces cerevisiae, is a member of ATP-binding cassette (ABC) superfamily. Pdr5p shares similar mechanisms substrate recognition and transport with human MDR1-Pgp, despite an inverted topology transmembrane domains. The hexahistidine-tagged transporter was highly overexpressed yeast strains where other ABC genes have been deleted. After solubilization purification, 160-kDa recombinant has reconstituted into lipid bilayer. Controlled detergent...
The search for new therapeutic strategies leishmaniasis treatment is essential due to the side effects of available drugs and increasing incidence resistance them. Marine sponges use chemical compounds as a defense mechanism, several them present interesting pharmacological properties. aim this study was evaluate in vitro activity aqueous extract marine sponge Dercitus (Stoeba) latex against Leishmania amazonensis. MIC toxicity mammal cells were evaluated through broth microdilution assays....
Calcineurin inhibitors - such as the clinically used drug tacrolimus are active against important fungal pathogens, particularly when combined with azoles. However, has not been tested sporotrichosis, an endemic subcutaneous mycosis worldwide distribution. Here, we evaluated activity of and cyclosporine A in vitro monotherapy combination itraconazole or fluconazole yeasts Sporothrix brasiliensis S. schenckii, main sporotrichosis agents Brazil. We also analyzed effect treatment on...
Mucormycosis is considered concerning invasive fungal infections due to its high mortality rates, difficult diagnosis and limited treatment approaches. Mucorales species are highly resistant many antifungal agents the search for alternatives an urgent need. In present study, a library with 400 compounds called Pandemic Response Box® was used four were identified: alexidine three non-commercial molecules. These showed anti-biofilm activity, as well alterations in morphology cell wall plasma...
Abstract Introduction – Phytochemical and biological studies carried out on Copaifera species showed that their oleoresins isolated compounds have various activities. Objective The aims of this work were (i) to analyse the oleoresin by gas chromatography–mass spectrometry, (ii) isolate diterpenic acids from high‐speed countercurrent chromatography (HSCCC) (iii) determine rhodamine 6G Pdr5p activity these acids. Methodology HSCCC was used for preparative separation diterpenes. Spectroscopic...
Chemical investigation of a propolis sample collected in Honduras has led to the isolation new (E,Z)-cinnamyl cinnamate (2) together with 14 known compounds: 6 cinnamic ester derivatives, 2 flavanones, 1 chalcone, triterpenes, and 3 aromatic acids. Structural determination was accomplished by spectroscopic analysis, particularly two-dimensional (2D) nuclear magnetic resonance (NMR) electrospray ionization-tandem mass spectrometry (ESI-MS/MS) techniques. Futhermore, we checked ability extract...
ABSTRACT Invasive Candida albicans infections are a serious health threat for immunocompromised individuals. Fluconazole is most commonly used to treat these infections, but resistance due the overexpression of multidrug efflux pumps grave concern. This study evaluated ability five synthetic organotellurium compounds reverse fluconazole C. clinical isolates. Compounds 1 4, at <10 μg/ml, ameliorated Saccharomyces cerevisiae strains overexpressing major Cdr1p and Mdr1p, whereas compound 5...
Candida species are fungal pathogens known to cause a wide spectrum of diseases, and albicans glabrata the most common associated with invasive infections. A concerning aspect candidiasis is emergence resistant isolates, especially those highly fluconazole, first choice treatment for these Fungal sphingolipids have been considered potential target new therapeutic approaches some inhibitors already tested against pathogenic fungi. The present study therefore aimed evaluate action two...
Scedosporium and Lomentospora species are opportunistic filamentous fungi that cause localized disseminated infections in immunocompetent immunocompromised patients. These considered resistant due to their low susceptibility most current antifungal agents used healthcare settings. The search for new compounds could work as promising candidate drugs is an increasing field of interest. In this context, the present study we screened Pandemic Response Box ® library (Medicines Malaria Venture...
Resistance to fluconazole, a commonly used azole antifungal, is challenge for the treatment of fungal infections. can be mediated by overexpression ABC transporters, which promote drug efflux that requires ATP hydrolysis. The Pdr5p transporter Saccharomyces cerevisiae well-known model study this mechanism antifungal resistance. present investigated effects 13 synthetic compounds on Pdr5p.Among tested compounds, four contained tellurium-butane group and shared structural similarities were...