Schistosomiasis continues to be a significant public health problem. This disease affects 200 million people worldwide and almost 800 are at risk of acquiring the infection. Although vaccine development against this has experienced more failures than successes, encouraging results have recently been obtained using membrane-spanning protein antigens from tegument Schistosoma mansoni. Our group identified Sm29, another antigen that is present adult worm surface. In study, we investigated...

Soybean (Glycine max [L.] Merr.) is a major crop in animal feed and human nutrition, mainly for its rich protein oil contents. The remarkable rise soybean transcriptome studies over the past 5 years generated an enormous amount of RNA-seq data, encompassing various tissues, developmental conditions genotypes. In this study, we have collected data from 1298 publicly available samples, processed raw sequencing reads mapped them to reference genome systematic fashion. We found that 94%...

Soybean (Glycine max) is a major legume crop worldwide, providing critical source of protein and oil. The release the soybean genome fuelled several transcriptome projects comprising multiple developmental stages environmental conditions. Nevertheless, global transcriptional patterns embryonic axes during germination remain unknown. Here we report analysis ~1.58 billion RNA-Seq reads from at five stages. Our results support early activation processes that are for germination, such as...

Plant-bacteria associations have been extensively studied for their potential in increasing crop productivity a sustainable manner. Serratia marcescens is species of Enterobacteriaceae found wide range environments, including soil.Here we describe the genome sequencing and assessment plant growth-promoting abilities S. UENF-22GI, strain isolated from mature cattle manure vermicompost. In vitro, UENF-22GI able to solubilize P Zn, produce indole compounds (likely IAA), colonize hyphae counter...

Background The parasitic trematode Schistosoma mansoni is one of the major causative agents human schistosomiasis, which afflicts 200 million people worldwide. Praziquantel remains main drug used for schistosomiasis treatment, and reliance on single therapy has been prompting search new therapeutic compounds against this disease. Our group demonstrated that heme crystallization into hemozoin (Hz) within S. gut a detoxification route with lipid droplets involved in process acting as potential...

Blood-feeding mosquitoes are exposed to high levels of heme, the product hemoglobin degradation. Heme is a pro-oxidant that influences variety cellular processes. We performed global analysis heme-regulated Aedes aegypti (yellow fever mosquito) transcriptional changes better understand influence on mosquito physiology at molecular level. observed an iron- and reactive oxygen species (ROS)-independent signaling induced by heme comprised genes related redox metabolism. By modulating abundance...

Soybean is a crucial crop worldwide, used as source of food, feed, and industrial products due to its high protein oil content. Previously, the rapid accumulation soybean RNA-seq data in public databases computational challenges processing raw motivated us develop Expression Atlas, gene expression database over thousand samples. Over past few years, our has allowed researchers explore profiles important families, discover genes associated with agronomic traits, understand transcriptional...

Genetic discontinuity represents abrupt breaks in genomic identity among species. Advances genome sequencing have enhanced our ability to track and characterize genetic bacterial populations. However, exploring the degree which diversity exists as a continuum or sorted into discrete readily defined species remains challenge microbial ecology. Here, we aim quantify ( $$\delta$$ ) investigate how this metric is related We harness dataset comprising 210,129 genomes systematically explore...

Abstract The genome sequence of Aedes aegypti was recently reported. A significant amount Expressed Sequence Tags (ESTs) were sequenced to aid in the gene prediction process. In present work we describe an integrated analysis genomic and EST data, focusing on genes with preferential expression larvae (LG), adults (AG) both stages (SG). total 913 (5.4% transcript complement) are LG, including ion transporters cuticle proteins that important for homeostasis defense. From a starting set 245...

Identification of essential genes is critical to understanding the physiology a species, proposing novel drug targets and uncovering minimal gene sets required for life. Although several organisms have been determined using large‐scale mutagenesis techniques, systematic studies addressing their conservation, genomic context functions remain scant. Here we integrate 17 from genome‐wide in vitro screenings three collections growth vivo , encompassing 15 Bacteria one Archaea. We refine...

Abstract Using sensitive sequence profile analysis, we identify a hitherto uncharacterized cysteine-rich, transmembrane (TM) module, CYSTM, found in wide range of tail-anchored membrane proteins across eukaryotes. This superfamily includes Schizosaccharomyces Uvi15, Arabidopsis PCC1, Digtaria CDT1 and Saccharomyces YDL012C YDR210W, which have all been implicated resistance/response to stress or pathogens. Based on the pattern conserved cysteines data from different chemical genetics studies,...

Gene regulatory networks (GRNs) evolve as a result of the coevolutionary processes acting on transcription factors (TFs) and cis-regulatory modules they bind. The zinc-finger TF zelda (zld) is essential for maternal-to-zygotic transition (MZT) in Drosophila melanogaster, where it directly binds over thousand to regulate chromatin accessibility. D. melanogaster displays long germ type embryonic development, all segments are simultaneously generated along whole egg. However, remains unclear if...

Klebsiella aerogenes is an important pathogen in healthcare‐associated infections. Nevertheless, comparison to other clinically pathogens, K. population structure, genetic diversity, and pathogenicity remain poorly understood. Here, we elucidate clonal complexes ( CC s) genomic features associated with resistance virulence. We present a detailed description of the structure based on 97 publicly available genomes by using both multilocus sequence typing single‐nucleotide polymorphisms...

Multidrug-resistant (MDR) Klebsiella pneumoniae (Kp) is a major bacterial pathogen responsible for hospital outbreaks worldwide, mainly via the spread of high-risk clones and epidemic resistance plasmids. In this study, we evaluated molecular epidemiology β-lactam mechanisms MDR-Kp strains isolated in Brazilian academic care hospital. We used whole-genome sequencing to study drug their relationships with K. carbapenemase-producing (KPC) Kp outbreak. Forty-three were collected between 2003...

Background Schistosoma mansoni is the major causative agent of schistosomiasis. The parasite takes advantage host signals to complete its development in human body. Tumor necrosis factor-alpha (TNF-α) a cytokine involved skin inflammatory responses, and although effect on adult parasite's metabolism egg-laying process has been previously described, comprehensive assessment TNF-α pathway downstream molecular effects lacking. Methodology/Principal Findings In present work we describe possible...

The ubiquitin system (Ub-system) can be defined as the ensemble of components including Ub/ubiquitin-like proteins, their conjugation and deconjugation apparatus, binding partners proteasomal system. While several studies have concentrated on structure-function relationships evolution individual Ub-system, a study whole is largely lacking. Using numerous genome-scale datasets, we assemble for first time comprehensive reconstruction budding yeast revealing static dynamic properties. We...

F-box proteins constitute a large gene family that regulates processes from hormone signaling to stress response. are the substrate recognition modules of SCF E3 ubiquitin ligases. Here we report very distinct trends in size, duplication, synteny and transcription genes two nitrogen-fixing legumes, Glycine max (soybean) Medicago truncatula (alfafa). While soybean FBX emerged mainly through segmental duplications (including whole-genome duplications), M. genome is dominated by...