A5058450175- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Caveolin-1 and cellular processes
- Congenital heart defects research
- Virus-based gene therapy research
- Lipid metabolism and disorders
- Congenital Heart Disease Studies
- Immune cells in cancer
- Axon Guidance and Neuronal Signaling
- Cardiovascular Effects of Exercise
- Lymphatic System and Diseases
- Cardiac Fibrosis and Remodeling
- Cellular Mechanics and Interactions
- Viral Infections and Immunology Research
- Cardiomyopathy and Myosin Studies
- 3D Printing in Biomedical Research
- Bone and Joint Diseases
- Advanced Fluorescence Microscopy Techniques
- Mesenchymal stem cell research
- Clusterin in disease pathology
- Venous Thromboembolism Diagnosis and Management
- Nerve injury and regeneration
- Electrospun Nanofibers in Biomedical Applications
International Centre for Genetic Engineering and Biotechnology
2012-2022
ETH Zurich
2021-2022
Zero to Three
2019
University of Trieste
2012-2018
Azienda Sanitaria Universitaria Integrata di Trieste
2018
Scuola Superiore Sant'Anna
2010
New York Medical College
2010
University of Oklahoma
2010
University of Oklahoma Health Sciences Center
2010
National Center for Genetic Engineering and Biotechnology
2007
Cardiomyocyte proliferation stops at birth when the heart is no longer exposed to maternal blood and, likewise, regulatory T cells (Tregs) that are expanded promote tolerance towards fetus. Here, we report a role of Tregs in promoting cardiomyocyte proliferation. Treg-conditioned medium promotes proliferation, similar serum from pregnant animals. Proliferative cardiomyocytes detected mothers, and Treg depletion during pregnancy decreases both fetal after myocardial infarction results...
Vascular endothelial growth factor (VEGF)-B selectively binds VEGF receptor (VEGFR)-1, a that does not mediate angiogenesis, and is emerging as major cytoprotective factor.To test the hypothesis VEGF-B exerts non-angiogenesis-related cardioprotective effects in nonischemic dilated cardiomyopathy.AAV-9-carried VEGF-B(167) cDNA (10(12) genome copies) was injected into myocardium of chronically instrumented dogs developing tachypacing-induced cardiomyopathy. After 4 weeks pacing, green...
Experimental and clinical evidence indicate that bone marrow cells participate in the process of new blood vessel formation. However, molecular mechanisms underlying their recruitment exact role are still elusive. Here, we show recruited to sites neoangiogenesis through neuropilin-1 (NP-1) receptor they essential for maturation activated endothelium formation arteries mice. By exploiting adeno-associated virus vector–mediated, long-term vivo gene expression, 165-aa isoform VEGF, which both...
Members of the vascular endothelial growth factor (VEGF) family are among most promising cytokines to induce neovascularization ischaemic tissues; however, their unregulated expression often results in major undesired effects. Here, we describe properties inducible vectors based on adeno-associated virus (AAV), allowing precise control VEGF expression, and exploit these define kinetics angiogenic response elicited by factor. Based a tetracycline-inducible transactivator, designed an AAV...
The aim of this study was to evaluate a novel animal model bisphosphonates-associated osteonecrosis, which realistically recapitulates the same pathological human condition. Five Wistar rats were given intravenous zoledronic acid 0.04 mg once week for 5 weeks. After 2 weeks, animals underwent extraction an upper molar, producing 4 mm-diameter bone defect on site. 7 weeks from extraction, clinically examined and scintigraphy carried out. additional week, killed both Computerized Tomography...
Improving tumor perfusion, thus tempering tumor-associated hypoxia, is known to impair cancer progression. Previous work from our laboratory has shown that VEGF-A165 and semaphorin 3A (Sema3A) promote vessel maturation through the recruitment of a population circulating monocytes expressing neuropilin-1 (Nrp1) receptor (Nrp1-expressing monocytes; NEM). Here, we define characteristics bone marrow NEMs assess whether these cells might represent an exploitable tool induce maturation. Gene...
Rationale Alveolar type II (ATII) cells act as adult stem contributing to alveolar I (ATI) cell renewal and play a major role in idiopathic pulmonary fibrosis (IPF), supported by familial cases harbouring mutations genes specifically expressed these cells. During IPF, ATII lose their regenerative potential aberrantly express pathways epithelial–mesenchymal transition (EMT). The microRNA miR-200 family is downregulated but its effect on human IPF remains unproven. We wanted 1) evaluate the...
Endothelial senescence entails alterations of the healthy cell phenotype, which accumulate over time and contribute to cardiovascular disease. Mechanical aspects regulating adhesion, force generation, response flow senescence-associated drift; however, they remain largely unexplored. Here, we exploit microscopy resolve variations anchoring substrate tractions generated upon aging in nanonewton (nN) range. Senescent endothelial cells display a multifold increase levels basal adhesion...
Despite the high prevalence of ischemic heart diseases worldwide, no antibody-based treatment currently exists. Starting from evidence that a specific isoform Bone Morphogenetic Protein 1 (BMP1.3) is particularly elevated in both patients and animal models myocardial infarction, here we assess whether its inhibition by monoclonal antibody reduces cardiac fibrosis. We find this collagen deposition cross-linking, paralleled enhanced cardiomyocyte survival, vivo primary cultures cells....
Although the angiogenic effect of vascular endothelial growth factor (VEGF) is widely recognized, a central question concerns whether vessels formed on its overexpression effectively increase tissue perfusion in vivo. To explore this issue, here we exploit AAV vectors to obtain prolonged expression VEGF and angiopoietin-1 (Ang1) rat skeletal muscle. Over period 6 months, muscle blood flow (MBF) permeability were measured by positron emission tomography single-photon computed tomography,...
Functional recovery after peripheral nerve injury depends on both improvement of regeneration and prevention denervation-related skeletal muscle atrophy. To reach these goals, in this study we overexpressed vascular endothelial growth factor (VEGF) by means local gene transfer with adeno-associated virus (AAV). Local the regenerating was obtained reconstructing a 1-cm-long rat median defect using vein segment filled fibers that have been previously injected either AAV2-VEGF or AAV2-LacZ,...
Diagnosis of Arrhythmogenic CardioMyopathy (ACM) is challenging and often late after disease onset. No circulating biomarkers are available to date. Given their involvement in several cardiovascular diseases, plasma microRNAs warranted investigation as potential non-invasive diagnostic tools ACM. We sought identify differentially expressed ACM with respect Healthy Controls (HC) Idiopathic Ventricular Tachycardia patients (IVT), differential diagnosis. HC subjects were screened for plasmatic...
Abstract Myocardial regeneration is restricted to early postnatal life, when mammalian cardiomyocytes still retain the ability proliferate. The molecular cues that induce cell cycle arrest of neonatal towards terminally differentiated adult heart muscle cells remain obscure. Here we report miR-106b~25 cluster higher expressed in myocardium and decreases expression adulthood, especially under conditions overload, orchestrates transition cardiomyocyte hyperplasia hypertrophy by virtue its...
Abstract Background: Microsurgical tissue engineering is an emerging topic in regenerative medicine. Here we describe a new microsurgical model of bioengineering rats based on the use arterovenous loop (AV) implanted into commercially available crosslinked collagen/glycosaminoglycan template. Methods: The microvascular was created between femoral artery and vein covered by template folded onto itself. chamber isolated from outside outer silicon layer to impede ingrowth. Results : At 1‐month...
Endothelialization strategies aim at protecting the surface of cardiovascular devices upon their interaction with blood by generation and maintenance a mature monolayer endothelial cells. Rational engineering micro-topography luminal interface provides powerful access point to support survival living endothelium under challenging hemodynamic conditions created implant deployment function. Surface structuring protocols must however be adapted complex, non-planar architecture target device...
The data and information presented here refer to the research article entitled: "Reactivating endogenous mechanisms of cardiac regeneration via paracrine boosting with human amniotic fluid stem cell secretome" (Balbi et al., 2019, Apr 04). This dataset illustrates in vitro effect exerted by secretome on rodent neonatal cardiomyocytes, endothelial progenitors different subsets progenitor cells. Cytokine/chemokine profiling is provided as well. can provide useful insights regenerative medicine...
In regenerative medicine, new approaches are required for the creation of tissue substitutes, and interplay between different research areas, such as engineering, microsurgery gene therapy, is mandatory. this article, we report a modification published model based on an arterio-venous loop enveloped in cross-linked collagen-glycosaminoglycan template, which acts isolated chamber angiogenesis formation. order to foster formation within chamber, entails development vessels, wondered whether...
Thrombogenicity remains a major issue in cardiovascular implants (CVIs). Complete surficial coverage of CVIs by monolayer endothelial cells (ECs) prior to implantation represents promising strategy but is hampered the overall logistical complexity and high number required. Consequently, extensive cell expansion necessary, which may eventually lead replicative senescence. Considering that micro-structured surfaces with anisotropic topography promote endothelialization, we investigated impact...