A5069332095- Telomeres, Telomerase, and Senescence
- Wound Healing and Treatments
- Virus-based gene therapy research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Genetics, Aging, and Longevity in Model Organisms
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- Viral Infections and Immunology Research
- Cancer-related molecular mechanisms research
- Cardiomyopathy and Myosin Studies
- Potato Plant Research
- Mesenchymal stem cell research
- Body Contouring and Surgery
- Circadian rhythm and melatonin
- Neonatal Respiratory Health Research
- Edible Oils Quality and Analysis
- Glycogen Storage Diseases and Myoclonus
- Signaling Pathways in Disease
- Skin Protection and Aging
- Coronary Interventions and Diagnostics
- Mechanical Circulatory Support Devices
- Cytomegalovirus and herpesvirus research
- Electrospun Nanofibers in Biomedical Applications
International Centre for Genetic Engineering and Biotechnology
2014-2025
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
2021-2024
Austrian Cluster for Tissue Regeneration
2021-2024
Ludwig Boltzmann Gesellschaft
2021-2024
Ludwig Boltzmann Institute for Digital Health and Prevention
2022-2024
International Centre for Genetic Engineering and Biotechnology
2022
Temple University
2014
Aging is the main risk factor for many chronic degenerative diseases and cancer. Increased senescent cell burden in various tissues a major contributor to aging age-related diseases. Recently, new class of drugs termed senolytics were demonstrated extending healthspan, reducing frailty improving stem function multiple murine models aging. To identify novel more optimal senotherapeutic combinations, we established senescence associated β-galactosidase assay as screening platform rapidly that...
Rationale Alveolar type II (ATII) cells act as adult stem contributing to alveolar I (ATI) cell renewal and play a major role in idiopathic pulmonary fibrosis (IPF), supported by familial cases harbouring mutations genes specifically expressed these cells. During IPF, ATII lose their regenerative potential aberrantly express pathways epithelial–mesenchymal transition (EMT). The microRNA miR-200 family is downregulated but its effect on human IPF remains unproven. We wanted 1) evaluate the...
The spatial boundaries of tissue response to wounding are unknown. Here, we show that in mammals, the ribosomal protein S6 (rpS6) is phosphorylated skin injury, forming a zone activation surrounding region initial insult. This p-rpS6-zone forms within minutes after and present until healing complete. robust marker as it encapsulates features process, including proliferation, growth, cellular senescence, angiogenesis. A mouse model unable phosphorylate rpS6 shows an acceleration wound...
As mediators of intercellular communication, extracellular vesicles containing molecular cargo, such as microRNAs, are secreted by cells and taken up recipient to influence their cellular phenotype function. Here we report that cardiac stress-induced differential microRNA content, with miR-200c-3p being one the most enriched, in cardiomyocyte-derived mediates functional cross-talk endothelial cells. Silencing mice subjected chronic increased pressure overload resulted attenuated hypertrophy,...
Pulmonary fibrosis is a devastating disease, in which fibrotic tissue progressively replaces lung alveolar structure, resulting chronic respiratory failure. Alveolar type II cells act as epithelial stem cells, being able to transdifferentiate into I mediate gas exchange, thus contributing homeostasis and repair after damage. Impaired transdifferentiation emerging major pathogenetic mechanism driving both onset progression of the lung. Here, we show that endothelial secrete angiocrine factors...
Fibrosis is a hallmark in the pathogenesis of various diseases, with very limited therapeutic solutions. A key event fibrotic process expression contractile proteins, including α-smooth muscle actin (αSMA) by fibroblasts, which become myofibroblasts. Here, we report results high-throughput screening library approved drugs that led to discovery haloperidol, common antipsychotic drug, as potent inhibitor myofibroblast activation. We show haloperidol exerts its antifibrotic effect on primary...
Cardiovascular toxicity remains one of the most adverse side effects in cancer patients receiving chemotherapy. Extra-virgin olive oil (EVOO) is rich preventive polyphenols endowed with anti-inflammatory, anti-oxidant activities which could exert protective on heart cells. One very interesting derivative EVOO preparation represented by purified extracts from mill waste waters (OMWW) polyphenols. Here, we have investigated anti-cancer activity a OMWW preparation, named A009, when combined...
Abstract Nonhealing wounds place a significant burden on both quality of life affected patients and health systems. Skin substitutes are applied to promote the closure nonhealing wounds, although their efficacy is limited by inadequate vascularization. The stromal vascular fraction (SVF) from adipose tissue promising therapy overcome this limitation. Despite few successful clinical trials, its incorporation in routine has been hampered inconsistent results. All these studies concluded...
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ABSTRACT Proliferation of vascular smooth muscle cells (vSMCs) following injury is a crucial contributor to pathological remodelling. MicroRNAs (miRNAs) are powerful gene regulators and attractive therapeutic agents. Here, we aim systematically identify characterise miRNAs with potential in targeting aberrant vSMC proliferation. We performed high-throughput vitro screen using library 2042 human miRNA-mimics for their impact on proliferation identified seven novel antiproliferative i.e...
Successful infection with Human Papillomaviruses requires mitosis, when incoming viral genomes gain access to nuclear components. However, very little is known about how long HPV particles can remain infectious in non-dividing cells or which cellular compartments these viruses may reside. To investigate questions we have used BJ as a reversible model of senescence and show that HPV-16 only infect early-passage proliferating cells. Late-passage senescent are resistant infection, but this be...
A major obstacle in inducing therapeutic angiogenesis the heart is inefficient gene transfer to endothelial cells (ECs). Here, we identify compounds able enhance permissiveness of cardiac ECs adeno-associated virus (AAV) vectors, which stand as ideal tools for vivo delivery. We screened a library >1,500 US Food and Drug Administration (FDA)-approved drugs, combination with AAV ECs. Among top drugs increasing AAV-mediated transduction, found vatalanib, an inhibitor multiple tyrosine kinase...
Summary Therapies halting the progression of fibrosis are ineffective and limited. Activated myofibroblasts emerging as important targets in fibrotic diseases. Previously, we performed a high-throughput screen on lung fibroblasts subsequently demonstrated that inhibition myofibroblast activation is able to prevent bleomycin-treated mice. High-throughput screens an ideal method repurposing drugs, yet they contain intrinsic limitation, which size library itself. Here, exploited data from our...
Abstract Background Cardiovascular toxicities still remain one of the most undesirable side effects in cancer patients receiving chemotherapy, and cardiotoxicity has been detected associated with many therapeutic regimens. A number mechanisms are reported for these effects, some which related to inflammation, oxygen radical generation, mitochondrial damage. Extra-virgin olive oil (EVOO) is rich preventive polyphenols endowed anti-inflammatory, antioxidant activities could exert protective on...