A5000612605- Dental Implant Techniques and Outcomes
- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Bone Metabolism and Diseases
- Orthopaedic implants and arthroplasty
- Electrospun Nanofibers in Biomedical Applications
- Dental Radiography and Imaging
- Bone health and treatments
- Forensic Anthropology and Bioarchaeology Studies
- dental development and anomalies
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Bone and Dental Protein Studies
- Tendon Structure and Treatment
- Silk-based biomaterials and applications
- Shoulder Injury and Treatment
- Oral and Maxillofacial Pathology
- Wound Healing and Treatments
- Dental materials and restorations
- Bone health and osteoporosis research
- Osteoarthritis Treatment and Mechanisms
- Orthopedic Surgery and Rehabilitation
- Cleft Lip and Palate Research
- Orthodontics and Dentofacial Orthopedics
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
2016-2025
Ludwig Boltzmann Institute for Digital Health and Prevention
2015-2025
Medical University of Vienna
2016-2025
Austrian Cluster for Tissue Regeneration
2016-2025
University Clinic of Traumatology
2019-2025
Austrian Biolmaging - Verein zur innovativen, korrelativen, multimodalen Bildgebung in Österreich
2020-2021
Creative Commons
2018
University of Applied Sciences Technikum Wien
2011
Context: Established bone turnover markers do not reflect fracture risk in idiopathic male and premenopausal osteoporosis the role of microRNAs (miRNAs) these patients is currently unclear. miRNAs are a class small non-coding RNAs that regulate gene expression tissue homeostasis. They considered new endocrine regulators with promising potential as biomarkers. Objective: Evaluation circulating miRNA signatures female subjects postmenopausal osteoporotic low-traumatic fractures. Design,...
Abstract The introduction of two-photon polymerization (2PP) to the field tissue engineering and regenerative medicine (TERM) has led great expectations for production scaffolds with an unprecedented degree complexity tailorable architecture. Unfortunately, resolution size are usually mutually exclusive when using 2PP, resulting in a lack highly-detailed relevant clinical application. Through combination highly reactive photopolymer optimizing key printing parameters, we propose first time...
The assessment of bone quality and the prediction fracture risk in idiopathic osteoporosis (IOP) are complex prospects as mineral density (BMD) turnover markers (BTM) do not indicate fracture-risk. MicroRNAs (miRNAs) promising new biomarkers for diseases, but current understanding biological information contained variability miRNAs is limited. Here, we investigated association between serum-levels 19 miRNA to microstructure histomorphometry based upon biopsies µCT (9.3 μm) scans from 36...
Abstract Age‐induced decline in osteogenic potential of bone marrow mesenchymal stem cells (BMSCs) potentiates osteoporosis and increases the risk for fractures. Despite epidemiology studies reporting concurrent development vascular diseases elderly, underlying mechanisms vascular‐bone cross‐talk aging are largely unknown. In this study, we show that accelerated endothelial deteriorates tissue through paracrine repression Wnt‐driven‐axis BMSCs. Here, utilize physiologically aged mice...
Sclerostin is a Wnt signalling antagonist that controls bone metabolism. expressed by osteocytes and cementocytes; however, its role in the formation of dental structures remains unclear. Here, we analysed mandibles sclerostin knockout mice to determine influence on dimensions using histomorphometry micro-computed tomography (μCT) imaging. μCT histomorphometric analyses were performed first lower molar surrounding lacking functional gene wild-type controls. six animals each group revealed...
Background: Because of ongoing problems with anterior cruciate ligament (ACL) reconstruction, new approaches in the treatment ACL injuries, particularly strategies based on tissue engineering, have gained increasing research interest. To allow for regeneration, a structured scaffold that provides mechanical basis, has cells from different sources, and comprises as well biological factors is needed. Biological materials, biodegradable polymers, composite materials are being used tested...
Microcomputed tomography ( μ CT) is widely used for the study of mineralized tissues, but a similar use soft tissues hindered by their low X-ray attenuation. This limitation can be overcome recent development different staining techniques. Staining with Lugol’s solution, mixture one part iodine and two parts potassium iodide in water, stands out among these techniques its complexity cost. Currently, Lugol mostly anatomical examination tissues. In present study, we seek to optimize quality...
Abstract Background Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSC). 3D printing offers possibility to produce customized scaffolds for complex defects. The aim this study was compare potential human BMSC cultured as 2D monolayers or spheroids encapsulated in constructs 3D-printed poly-L-lactide-co-trimethylene carbonate and modified platelet lysate hydrogels (PLATMC-HPLG) regeneration. Methods PLATMC-HPLG...
The spatial boundaries of tissue response to wounding are unknown. Here, we show that in mammals, the ribosomal protein S6 (rpS6) is phosphorylated skin injury, forming a zone activation surrounding region initial insult. This p-rpS6-zone forms within minutes after and present until healing complete. robust marker as it encapsulates features process, including proliferation, growth, cellular senescence, angiogenesis. A mouse model unable phosphorylate rpS6 shows an acceleration wound...
Functionalizing biomaterials with conditioned media (CM) from mesenchymal stromal cells (MSC) is a promising strategy for enhancing the outcomes of guided bone regeneration (GBR). This study aimed to evaluate regenerative potential collagen membranes (MEM) functionalized CM human marrow MSC (MEM-CM) in critical size rat calvarial defects. MEM-CM prepared via soaking (CM-SOAK) or followed by lyophilization (CM-LYO) were applied Control treatments included native MEM, MEM (CEL) and no...
Bio-Gide® is a collagen membrane routinely used in guided bone regeneration. Recent studies have shown that this has osteoconductive properties, meaning it can support the growth of new bone. However, also been observed areas mineralized fibers which occur spontaneously and independently osteoblasts. To better understand how works, we established model using minced membranes to reduce active mineralization intact favor passive mineralization. We thus compared original with 5 mm calvarial...
Abstract Objectives This study aims to investigate the impact of pro-osteoblastogenic ERK-activated ribosomal S6 kinase ( Rsk2) on Tumor necrosis factor (TNF)α-induced bone loss in craniofacial system, focusing its role rheumatoid arthritis (RA). The objective is understand whether Rsk2 , previously shown have protective effects long bones against TNFα-induced resorption, exhibits similar region. Materials and Methods. compares mice with TNFα overexpression, knockout mice, a combination...
Introduction Bone healing is a well-orchestrated process involving various bone cells and signaling pathways, where disruptions can result in delayed or incomplete healing. MicroRNAs (miRNAs) are small non-coding RNAs capable of influencing cellular processes, including remodeling. Due to their biological relevance stable presence biofluids, miRNAs may serve as candidates for diagnosis prognosis The aim the study was investigate changes circulating blood during rat calvaria defects...
Spinal cord injury (SCI) is a complex clinical condition with wide range of permanent functional and neurological consequences. A prime factor limiting the patient’s quality life (QoL) difficulties in bladder function. Chronic animal models that help to develop novel therapeutic strategies are highly demanded, but their availability scarce frequently accompanied by substantial limitations. We want provide our detailed protocols allow full reproducibility model for investigating both acute...
Abstract The Upper Palaeolithic double burial of newborns and the single a ca. 3-month-old infant uncovered at Gravettian site Krems-Wachtberg, Austria, are paramount importance given rarity immature human remains from this time. Genome-wide ancient DNA shows that male infants grave earliest reported case monozygotic twins, while grave´s individual was their 3rd-degree relative. We assessed individuals´ age death by applying histological µCT inspection maxillary second incisors (i2) in...
Natural extracellular matrix-derived biomaterials from decellularized allogenic tissues are of increasing interest for tissue engineering because their structure and composition provide a complexity that is not achievable with current manufacturing techniques. The prerequisite to bring bench bedside as functional biomaterial the full removal cells while preserving most its native characteristics such composition. exceptionally dense articular cartilage, however, poses special challenge...
Abstract Objectives Collagen membranes are not limited to be occlusive barriers as they actively support bone regeneration. However, the impact of bone‐derived growth factors on their osteoconductive competence has been examined. Methods Twenty adult Sprague Dawley rats were included in study. Calvaria defects with a diameter five millimeter created. The defect was covered one layer collagen membrane previously soaked conditioned medium porcine chips or culture alone. After 4 weeks,...
The study evaluates the influence of two spacer settings and three resin luting materials on marginal internal fit polymer-infiltrated ceramic network (PICN) material crowns manufactured using a complete digital workflow.Optical impressions fifty identical dies were performed 3M scanner (software version 5.0.2). Twenty designed Ceramill Mind (version 3.4.10.1163), from which ten with setting 50 μm (G1) 80 (G2). Thirty (spacer μm) divided into groups corresponding to used as follows: RelyX...
Background: Bone mineral density at the humeral head is reduced in patients with chronic rotator cuff tears. loss associated repair failure after reconstruction. Bisphosphonates (eg, zoledronic acid) increase bone density. Hypothesis: Zoledronic acid improves of and biomechanical properties enthesis reconstruction tears rats. Study Design: Controlled laboratory study. Methods: A total 32 male Sprague-Dawley rats underwent unilateral (left) supraspinatus tenotomy delayed transosseous 3 weeks....
Local inflammation plays a pivotal role in the process of secondary damage after spinal cord injury. We recently reported that acute intravenous application extracellular vesicles (EVs) secreted by human umbilical mesenchymal stromal cells dampens induction inflammatory processes following traumatic However, systemic EVs is associated with delayed delivery to site injury and necessity for high doses reach therapeutic levels locally. To resolve these two constraints, we injected directly at...
Bone grafts can be engineered by differentiating human mesenchymal stromal cells (MSCs) via the endochondral and intramembranous ossification pathways. We evaluated effects of each pathway on properties bone their capacity to drive regeneration. Bone-marrow-derived MSCs were differentiated silk scaffolds into either hypertrophic chondrocytes (hyper) or osteoblasts (osteo) over 5 weeks in vitro cultivation, implanted subcutaneously for 12 weeks. The pathways’ constructs time with respect gene...
Background: Given the unsatisfactory results and reported drawbacks of anterior cruciate ligament (ACL) reconstruction, such as donor site morbidity limited choice grafts in revision surgery, new regenerative approaches based on tissue-engineering strategies are currently under investigation. Purposes: To determine (1) if a novel silk fiber–based ACL scaffold is able to initiate osteointegration femoral tibial bone tunnels vivo conditions (2) process will be improved by intraoperatively...