A5069201485- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Viral Infectious Diseases and Gene Expression in Insects
- Circular RNAs in diseases
- Alkaline Phosphatase Research Studies
- RNA modifications and cancer
- Virus-based gene therapy research
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Bone and Joint Diseases
- Renal and related cancers
- CRISPR and Genetic Engineering
- Immune responses and vaccinations
- Bone health and treatments
- Protein purification and stability
- Biochemical and Molecular Research
- Telomeres, Telomerase, and Senescence
- Sepsis Diagnosis and Treatment
- Acute Kidney Injury Research
- Bone health and osteoporosis research
- Heterotopic Ossification and Related Conditions
- Signaling Pathways in Disease
- Single-cell and spatial transcriptomics
- Chronic Kidney Disease and Diabetes
Austrian Centre of Industrial Biotechnology (Austria)
2016-2020
BOKU University
2015-2020
John Wiley & Sons (United States)
2015
Bioengineering Center
2015
National Center for Genetic Engineering and Biotechnology
2015
Abstract Aims Coronavirus disease 2019 (COVID-19) can lead to multiorgan damage. MicroRNAs (miRNAs) in blood reflect cell activation and tissue injury. We aimed determine the association of circulating miRNAs with COVID-19 severity 28 day intensive care unit (ICU) mortality. Methods results performed RNA-Seq plasma healthy controls (n = 11), non-severe 18), severe 18) patients selected 14 according cell- origin for measurement by reverse transcription quantitative polymerase chain reaction...
The plasma levels of tissue-specific microRNAs can be used as diagnostic, disease severity and prognostic biomarkers for chronic acute diseases drug-induced injury. Thereby, the combination diverse into biomarker signatures using multivariate statistics seems especially powerful from perspective tissue condition specific microRNA shedding plasma. Although next-generation sequencing (NGS) technology enables one to analyse circulating on a genome-scale level, it suffers potential biases (e.g.,...
Abstract Age‐induced decline in osteogenic potential of bone marrow mesenchymal stem cells (BMSCs) potentiates osteoporosis and increases the risk for fractures. Despite epidemiology studies reporting concurrent development vascular diseases elderly, underlying mechanisms vascular‐bone cross‐talk aging are largely unknown. In this study, we show that accelerated endothelial deteriorates tissue through paracrine repression Wnt‐driven‐axis BMSCs. Here, utilize physiologically aged mice...
Extracellular vesicles comprise an as yet inadequately investigated intercellular communication pathway in the field of early osteoarthritis. We hypothesised that small non-coding RNA expression pattern synovial fluid and plasma would change during progression experimental In this study, we conducted sequencing to provide a comprehensive overview temporal profiles transcripts carried by extracellular derived from for first time posttraumatic model equine Additionally, characterised...
<ns4:p>In contrast to traditional methods like real-time polymerase chain reaction, next-generation sequencing (NGS), and especially small RNA-seq, enables the untargeted investigation of whole RNAome, including microRNAs (miRNAs) but also a multitude other RNA species. With promising application RNAs as biofluid-based biomarkers, RNA-seq is method choice for an initial discovery study. However, presentation specific quality aspects data varies significantly between laboratories lacking...
Abstract Introduction Adult hypophosphatasia (HPP) patients present with diffuse heterogenous symptoms often mimicking rheumatological diseases or osteoporosis and therefore accompanied by delayed diagnosis. The aim of this study was to identify circulating miRNAs in adult HPP potential associations clinical patients’ characteristics. Methods We utilized untargeted miRNA biomarker discovery small RNA-sequencing investigate cell-free profiles 24 (pathogenic variant the ALPL gene, HPP-related...
Dysregulation of platelet function is causally connected to thrombus formation and cardiovascular diseases. Therefore, assessing reactivity crucial. However, current tests come with pitfalls, limiting clinical use. Plasma miRNA signatures have been suggested as novel biomarkers for predicting/diagnosing diseases monitoring antiplatelet therapy. Here, we provide results from a comprehensive study on the feasibility using circulatory miRNAs surrogate markers activation. We performed small...
Abstract MicroRNAs (miRNAs) are short non-coding RNAs that involved in post-transcriptional control of gene expression and might be used as biomarkers for diabetes-related complications. The aim this case–control study was to explore potential differences circulating miRNAs young individuals with long-duration type 1 diabetes (T1D) compared healthy controls, how identified expressed across different tissues. Twelve adolescents, age 15.0–17.9 years, T1D duration more than 8 years (mean 11.1...
Acute kidney injury (AKI) in sepsis patients increases patient mortality. Endothelial cells are important players the pathophysiology of sepsis-associated AKI (SA-AKI), yet knowledge regarding their spatiotemporal involvement coagulation disbalance and leukocyte recruitment is lacking. This study investigated identity kinetics responses different microvascular compartments cortex response to SA-AKI.
Abstract In the last decade cellular senescence, a hallmark of aging, has come into focus for pharmacologically targeting aging processes. Senolytics are one these interventive strategies that have advanced clinical trials, creating an unmet need minimally invasive biomarkers senescent cell load to identify patients at senotherapy. We created landscape miRNA and mRNA expression in five human types induced senescence in-vitro provide proof-of-principle evidence can track burden dynamically...
High-throughput siRNA screens were only recently applied to cell factories identify novel engineering targets which are able boost cells towards desired phenotypes. While libraries exist for model organisms such as mice, no CHO-specific library is publicly available, hindering the application of this technique CHO cells. The optimization these special interest, they main host production therapeutic proteins. Here, we performed a cross-species approach by applying mouse whole-genome cells,...
As Chinese Hamster Ovary (CHO) cells are the cell line of choice for production human-like recombinant proteins, there is interest in genetic optimization host lines to overcome certain limitations their growth rate and protein secretion. At same time, a detailed understanding these processes could be used advantage by identification marker transcripts that characterize states performance. In this context, microRNAs (miRNAs) exhibit robust correlation CHO were determined analyzing miRNA...
Endothelial cells in blood vessels the kidney exert different functions depending on (micro)vascular bed they are located in. The present study aimed to investigate microRNA and mRNA transcription patterns that underlie these differences. We zoomed microvascular compartments mouse renal cortex by laser microdissecting microvessels prior small RNA- RNA-sequencing analyses. By means, we characterized profiles of arterioles, glomeruli, peritubular capillaries, postcapillary venules....
MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications genomic sequencing data from the Chinese Hamster (CGR) and hamster ovary (CHO) cells provide new tools for discovery novel miRNAs this important production system. Version 20 miRNA registry miRBase contains 307 mature 200 precursor sequences CGR/CHO. We searched evolutionary conserved v20 recently published data, derived CHO cells, to further extend list known...
Abstract Extracellular vesicles (EVs) are crucial mediators of cell‐to‐cell communication in physiological and pathological conditions. Specifically, EVs released from the vasculature into blood were found to be quantitatively qualitatively different diseases compared healthy states. However, our understanding derived lymphatic system is still scarce. In this study, we mRNA microRNA (miRNA) expression vascular (BEC) (LEC) endothelial cells. After characterization by fluorescence‐triggered...
Abstract Patients with high‐grade gliomas are at high risk of venous thromboembolism (VTE). MicroRNAs (miRNAs) small non‐coding RNAs multiple roles in tumour biology, haemostasis and platelet function. Their association VTE glioma has not been comprehensively mapped so far. We thus conducted a nested case–control study within 152 patients WHO grade IV that had part prospective cohort on factors. At inclusion single blood draw was taken, were thereafter followed for maximum 2 years. During...
Abstract Introduction Adult hypophosphatasia (HPP) patients present with diffuse heterogenous symptoms often mimicking rheumatological diseases or osteoporosis and therefore accompanied by delayed diagnosis. The aim of this study was to identify circulating miRNAs in adult HPP potential associations clinical patients’ characteristics. Methods We utilized untargeted miRNA biomarker discovery small RNA-sequencing investigate cell-free profiles 24 (pathogenic variant the ALPL gene, HPP-related...
Obtaining highly productive Chinese hamster ovary (CHO)-cell clones for the production of therapeutic proteins relies on multiple time-consuming selection steps. Several CHO-cell strains with high degrees genomic and epigenetic variation are available. Each harbor potential advantages disadvantages any given product, particularly those considered difficult to express. A simple test system quickly assess compatibility cell line product may therefore prove useful. Transient plasmid...
Bone fragility is an adverse outcome of type 2 diabetes mellitus (T2DM). The underlying molecular mechanisms have, however, remained largely unknown. MicroRNAs (miRNAs) are short non-coding RNAs that control gene expression in health and disease states. aim this study was to investigate the genome-wide regulation miRNAs T2DM bone by analyzing serum tissue samples from a well-established rat model T2DM, Zucker Diabetic Fatty (ZDF) model. We performed small RNA-sequencing analysis detect...
Abstract Extracellular vesicles comprise an as yet inadequately investigated intercellular communication pathway in the field of early osteoarthritis. We hypothesised that small non-coding RNA expression pattern synovial fluid and plasma would change during progression experimental In this study, we used sequencing to provide a comprehensive overview temporal profiles transcripts carried by EVs derived from for first time post-traumatic model equine Additionally, characterised plasma-derived...
Type 2 diabetes mellitus (T2DM) increases the susceptibility of bone fragility. The underlying mechanisms have, however, remained largely unknown. MicroRNAs (miRNAs) are short single-stranded non-coding RNA molecules with utility as biomarkers due to their easy accessibility and stability in bodily fluids. Here, we aimed use an unbiased approach identify miRNAs dysregulated a polygenic mouse model T2DM. Genome-wide analysis serum, BM, from TallyHo/JngJ (TH) mice, which recapitulate T2DM...
Type 1 diabetes (T1D) represents a significant health burden worldwide, with associated complications including bone fragility. Current clinical methods and biomarkers for assessing predicting fracture risk in T1D are limited lack accuracy. MicroRNAs (miRNAs) have emerged as potential T1D-induced loss, although comprehensive profiling studies lacking. Previous investigations indicated link between dysregulated miRNA expression levels impaired T1D. Therefore, this study, we explored...
Hypophosphatasia (HPP) is characterized by low activity of tissue nonspecific alkaline phosphatase (TNSALP). The enzyme replacement therapy asfotase alfa has been approved for childhood-onset forms HPP. MicroRNAs (miRNAs) have emerged as a novel disease biomarker, with potential application in monitoring. Circulating miRNAs were analyzed at baseline, months 1, 2, 4, and 16 49-yr-old woman HPP, chronic musculoskeletal pain, non-traumatic fractures prior to therapy. Serum RNA was extracted...