A5070625240- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Multiple Myeloma Research and Treatments
- Protein purification and stability
- Medical and Biological Sciences
- Lung Cancer Treatments and Mutations
- Lung Cancer Diagnosis and Treatment
- Hemoglobin structure and function
- Cytomegalovirus and herpesvirus research
- Craniofacial Disorders and Treatments
- Cancer therapeutics and mechanisms
- Immunotherapy and Immune Responses
- Microbial Inactivation Methods
- Protein Degradation and Inhibitors
- T-cell and B-cell Immunology
- Peptidase Inhibition and Analysis
- Cell Adhesion Molecules Research
- Cardiac Arrest and Resuscitation
- Fungal and yeast genetics research
- Cleft Lip and Palate Research
- RNA Interference and Gene Delivery
- Phytochemical compounds biological activities
- Lung Cancer Research Studies
University of Arkansas for Medical Sciences
2018-2025
The University of Queensland
2015-2022
University of Newcastle Australia
2002
The University of Texas Health Science Center at San Antonio
2002
Predictable control of gene expression is necessary for the rational design and optimization cell factories. In yeast Saccharomyces cerevisiae, promoter one most important tools available controlling expression. However, complex patterns promoters have not been fully characterised compared on different carbon sources (glucose, sucrose, galactose ethanol) across diauxic shift in glucose batch cultivation. These conditions are importance to factory because they commonly used encountered...
Traumatic brain injury (TBI) attenuates the cerebral vasodilation to hypercapnia. Cortical spreading depression (CSD) also transiently reduces hypercapnic vasodilation. The authors sought determine whether CSD elicited by a controlled cortical impact (CCI) masks true effect of TBI on vasodilation, and nitric oxide (NO) donor can reverse attenuation following CCI.Anesthetized rats underwent moderate CCI. Cerebral blood flow was monitored with laser Doppler flowmetry response hypercapnia...
✓ Various combinations of cranial remodeling techniques are used in an attempt to provide optimal cosmetic results and reduce possible sequelae associated with craniosynostosis. One element deformity that is difficult correct directly overly flattened area such as found the parietal sagittal synostosis, unilaterally lambdoid or even severe positional molding. The authors present a novel application for recontouring bone, namely multiple-revolution spiral osteotomy. advantages this technique...
Abstract We previously demonstrated that increased monoclonal antibody productivity in dihydrofolate reductase (DHFR)-amplified CHO cells correlates with phosphorylated transcription factor-cytomegalovirus (CMV) promoter interactions. In this article, we extend the characterization to include CMV methylation and its influence on NFκB CREB1 factor binding two families of DHFR-amplified cell lines. was determined using bisulfite sequencing. To overcome Sanger-sequencing limitations due high CG...
PurposeOsimertinib is a third-generation EGFR-TKI and preferred first-line (1L) treatment for EGFR positive (EGFR+) metastatic non-small cell lung cancer (mNSCLC). This study compared real-world clinical outcomes of 1L osimertinib versus 1st or 2nd generation EGFR-TKIs (1/2G-TKIs) in patients with mNSCLC.MethodsNine academic centers the US participated retrospective cohort study. Patients aged ≥18 years EGFR+ mNSCLC treated were included. Clinical included progression-free survival (rwPFS),...
Chinese hamster ovary (CHO) cells are the industry standard cell line for high yield expression of biopharmaceuticals. However, leveraging influencers specific productivity to expedite generation clones remains a challenge. One factor impacting is strong, constitutive activity CMV promoter used in most vectors transgene applications. In this study, we sought understand influence DNA methylation along driving transcription higher vs lower lines. Bisulfite pyrosequencing was characterize...