A5101774330- Nitric Oxide and Endothelin Effects
- Traumatic Brain Injury and Neurovascular Disturbances
- Neurological Disease Mechanisms and Treatments
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Neuroinflammation and Neurodegeneration Mechanisms
- Spinal Fractures and Fixation Techniques
- Spine and Intervertebral Disc Pathology
- Prostate Cancer Treatment and Research
- Advanced MRI Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Spinal Dysraphism and Malformations
- Renin-Angiotensin System Studies
- Spinal Cord Injury Research
- Cervical and Thoracic Myelopathy
- Fungal Biology and Applications
- Bladder and Urothelial Cancer Treatments
- Hemoglobin structure and function
- Cardiac Ischemia and Reperfusion
- Cardiac Arrest and Resuscitation
- Cancer-related molecular mechanisms research
- Electron Spin Resonance Studies
- Cancer survivorship and care
- Intracerebral and Subarachnoid Hemorrhage Research
- Prostate Cancer Diagnosis and Treatment
- Advanced Neuroimaging Techniques and Applications
Fujian Agriculture and Forestry University
2022-2025
University of Washington
2012-2024
Neurological Surgery
2001-2024
South China Agricultural University
2024
Wenzhou Medical University
2007-2022
First Affiliated Hospital of Wenzhou Medical University
2007-2022
Budapest University of Technology and Economics
2021
Harborview Medical Center
2010-2020
Seattle University
2020
Nanyang Medical College
2019
Cyclo-oxygenase-2 (COX-2), a rate-limiting enzyme for prostanoid synthesis, is induced during inflammation and participates in inflammation-mediated cytotoxicity. Cerebral ischemia followed by an inflammatory reaction that plays role the evolution of tissue damage. We studied whether COX-2 after cerebral if so, such expression contributes to ischemic brain The middle artery was occluded rats, area sampled analysis 3–96 hr later. mRNA determined competitive reverse-transcription PCR....
Inducible nitric oxide synthase (iNOS), an enzyme that produces toxic amounts of oxide, is expressed in a number brain pathologies, including cerebral ischemia. We used mice with null mutation the iNOS gene to study role ischemic damage. Focal ischemia was produced by occlusion middle artery (MCA). In wild-type mice, mRNA expression post-ischemic begun between 24 and 48 hr peaked at 96 subsided 7 d after MCA occlusion. induction associated protein enzymatic activity. contrast, lacking did...
Cerebral ischemia is followed by a local inflammatory response that thought to participate in the extension of tissue damage occurring postischemic period. However, mechanisms whereby inflammation contributes progression have not been fully elucidated. In models inflammation, expression inducible isoform nitric oxide synthase (iNOS) responsible for cytotoxicity through production large amounts (NO). this study, therefore, we sought establish whether iNOS expressed ischemic brain. Rats were...
Despite modern antiseptic techniques, surgical site infection (SSI) remains a leading complication of surgery. However, the origins SSI and high rates antimicrobial resistance observed in these infections are poorly understood. Using instrumented spine surgery as model clean (class I) skin incision, we prospectively sampled preoperative microbiomes postoperative isolates cohort 204 patients. Combining multiple forms genomic analysis, correlated identity, anatomic distribution, profiles...
Background and Purpose We investigated whether inducible nitric oxide synthase (iNOS) is expressed after transient cerebral ischemia and, if so, we sought to define the temporal profile cellular localization of expression role iNOS in mechanism ischemic brain injury. Methods The middle artery rats was occluded for 2 hours by an intraluminal filament. occurrence reperfusion confirmed laser-Doppler flowmetry (n=5). message neocortex determined reverse-transcription polymerase chain reaction....
Focal cerebral ischemia is associated with expression of both inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), enzymes whose reaction products contribute to the evolution ischemic brain injury. We tested hypothesis that, after ischemia, (NO) produced by iNOS enhances COX-2 activity, thereby increasing toxic potential this enzyme. Cerebral was middle artery occlusion in rats or mice. Twenty-four hours rats, iNOS-immunoreactive neutrophils were observed close proximity...
We studied the effect of focal cerebral ischemia on inducible (iNOS) and constitutive (cNOS) nitric oxide synthase enzymatic activities in affected brain. The middle artery (MCA) was occluded spontaneously hypertensive rats. Animals were killed 1, 2, 4, 7 days later. cNOS iNOS determined infarcted cortex using assay Bredt Snyder. assayed presence calcium, whereas absence calcium tetrahydrobiopterin. validity verified rats treated with bacterial lipopolysaccharide. In these animals, magnitude...
We studied whether administration of nitric oxide (NO) donors reduces the ischemic damage resulting from middle cerebral artery (MCA) occlusion in spontaneously hypertensive rats (SHRs). In halothane-anesthetized and ventilated SHRs, MCA was occluded. CBF monitored using a laser-Doppler flowmeter. Three to five minutes after occlusion, NO sodium nitroprusside (SNP; 3 mg/kg/h) or 3-morpholino-sydnonimine (SIN 1; 1.5–6 were administered into carotid for 60 min. As control, effect papaverine...
We studied the role of amyloid precursor protein (APP) in ischemic brain damage using transgenic mice overexpressing APP. The middle cerebral artery (MCA) was occluded FVB/N expressing APP 695 .SWE (Swedish mutation) and nontransgenic littermates. Infarct volume (cubic millimeters) assessed 24 hr later thionin-stained sections. infarct produced by MCA occlusion enlarged transgenics (+32 ± 6%; n = 12; p < 0.05; t test). Measurement ELISA revealed that, although relatively high levels Aβ...
Background and Purpose We studied whether the inducible nitric oxide synthase (iNOS) inhibitor aminoguanidine reduces focal cerebral ischemic damage in a relatively noninvasive stroke model which rat middle artery (MCA) is occluded using an intravascular filament. Methods In rats anesthetized with halothane, nylon filament was advanced into internal carotid until its tip origin of MCA. The left place for 2 hours then withdrawn. Twenty-four later, received intraperitoneal injections (100...
We used the nitric oxide (NO) donor sodium nitroprusside (SNP) and NO synthase inhibitor nitro-l-arginine methyl ester (l-NAME) to study role of in ischemic damage produced by occlusion rat middle cerebral artery (MCA). After MCA occlusion, intracarotid administration SNP (2.5 mg kg h−2 for 1 h) enhanced recovery neocortical blood flow EEG reduced cortical infarct size 76 ± 2% (p 0.01; n = 5). In contrast, l-NAME (10 h−2) worsened CBF increased (+60 16%; p < 0.05; The findings indicate that...
Object Symptomatic thoracic disc herniations (TDHs) are relatively uncommon and typically treated with an anterior approach. Various posterior surgical approaches have been developed to treat TDH, but the gold standard remains transthoracic decompression. Certain patients comorbidities herniation aspects that not optimally A transfacet pedicle-sparing approach was first described in 1995, outcomes complications well described. The objective of this study assess a consecutive series TDH...
Prostate cancer is one of the most commonly diagnosed cancers in men worldwide. Currently available therapies for metastatic prostate are only marginally effective. Therefore, new therapeutic agents urgently needed to improve patient outcome. Isoalantolactone (IATL), an active sesquiterpene naturally present many vegetables and medicinal plants, known induce cell death apoptosis various lines. Nevertheless, antitumor mechanisms initiated by IATL cells have not been fully defined.Cell...
Study Design. The enhanced perioperative care (EPOC) program is an institutional quality improvement initiative. We used a historically controlled study design to evaluate patients who underwent major spine surgery before and after the implementation of EPOC program. Objective. To determine whether multidisciplinary was associated with in clinical financial outcomes for elective adult patients. Summary Background Data. recovery (ERAS) programs successfully implemented hip knee replacement...
Abstract Flow‐sensitive alternating inversion recovery (FAIR) is a recently introduced MRI technique for assessment of perfusion that uses blood water as an endogenous contrast agent. To characterize the FAIR signal dependency on spin tagging time (inversion (Tl)) and to validate cerebral flow (CBF) quantification, studies were conducted rat brain at 9.4 T using conventional gradient‐recalled echo sequence. The 7 1 , cortex was found be 1.9 2.2 s, respectively, used CBF calculations. At...
We studied whether delayed posttreatment with the nitric oxide donor 3-morpholinosydnonimine (SIN-1) is effective in reducing size of infarct produced by occlusion middle cerebral artery (MCA) spontaneously hypertensive rats (SHRs). SHRs were anesthetized halothane and intubated transorally. The left MCA was occluded at level inferior vein. Cerebral blood flow (CBF) monitored ischemic hemisphere a laser-Doppler flowmeter, an electroencephalogram (EEG) recorded. SIN-1 infused into internal...
The transcription factor interferon regulatory 1 (IRF-1) is involved in the molecular mechanisms of inflammation and apoptosis, processes that contribute to ischemic brain injury. In this study, induction IRF-1 response cerebral ischemia its role injury were investigated. gene expression was markedly upregulated within 12 h occlusion middle artery C57BL/6 mice. reached a peak 4 d after (6.0 ± 1.8-fold; P &lt; 0.001) restricted regions brain. volume reduced by 23 3% IRF-1+/− 46 9%...
OBJECTIVE The purpose of this study was to compare total cost and length stay (LOS) between spine surgery patients enrolled in an enhanced perioperative care (EPOC) pathway receiving traditional (TRDC). METHODS All candidates were screened for inclusion the EPOC pathway. This cohort compared a retrospective who received TRDC concurrent group met criteria but did not receive (no [NOPC] group). Direct indirect costs as well hospital intensive LOSs analyzed 3 groups. RESULTS Total after...