A5077722261- Glioma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Fibroblast Growth Factor Research
- Angiogenesis and VEGF in Cancer
- Phagocytosis and Immune Regulation
- Cell Image Analysis Techniques
- Extracellular vesicles in disease
- Cutaneous lymphoproliferative disorders research
- Zebrafish Biomedical Research Applications
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Acute Myocardial Infarction Research
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Simulation-Based Education in Healthcare
- Nanoparticle-Based Drug Delivery
- Circular RNAs in diseases
- Transplantation: Methods and Outcomes
- T-cell and Retrovirus Studies
- Brain Metastases and Treatment
Medical University of Vienna
2010-2024
Comprehensive Cancer Center Vienna
2020-2023
St Anna Children's Hospital
2014-2022
St. Anna Children's Cancer Research Institute
2014-2018
Dendritic cells (DCs) are antigen-presenting that capable of priming anti-tumor immune responses, thus serving as attractive tools to generate tumor vaccines. In this multicentric randomized open-label phase II study, we investigated the efficacy vaccination with lysate-charged autologous DCs (Audencel) in newly diagnosed glioblastoma multiforme (GBM). Patients aged 18 70 years histologically proven primary GBM and resection at least 70% were 1:1 standard care (SOC) or SOC plus (weekly...
Audencel is a dendritic cell (DC)-based cellular cancer immunotherapy against glioblastoma multiforme (GBM). It characterized by loading of DCs with autologous whole tumor lysate and in vitro maturation via "danger signals". The recent phase II "GBM-Vax" trial showed no clinical efficacy for as assessed progression-free overall survival all patients. Here we present immunological research accompanying the focus on immune system factors related to outcome Audencel's effect system....
Extracorporeal membrane oxygenation (ECMO) is gaining importance in the perioperative management of lung transplant patients. To date, ideal substance for anticoagulation ECMO patients still a matter debate. In this study, we describe our experience with use low molecular weight heparin (LMWH) comparison unfractioned (UFH) undergoing support. We retrospectively analyzed data from all who underwent support at institution between 2013 and 2017. Bleeding events served as primary outcome...
Abstract Glioblastoma is the most dangerous brain cancer. One reason for glioblastoma's aggressiveness are glioblastoma stem‐like cells. To target them, a number of markers have been proposed ( CD 133, 44, 15, A2B5, 36, CXCR 4, IL 6R, L1 CAM , and ITGA 6). A comprehensive study co‐expression patterns them has, however, not performed so far. Here, we mapped multidimensional profile these stemness‐associated molecules. Gliomaspheres – an established model cells were used. Seven different...
Glioblastoma (GBM) is characterized by a particularly invasive phenotype, supported oncogenic signals from the fibroblast growth factor (FGF)/ FGF receptor (FGFR) network. However, possible role of FGFR4 remained elusive so far. Several transcriptomic glioma datasets were analyzed. An extended panel primary surgical specimen-derived and immortalized GBM (stem)cell models original tumor tissues screened for expression. engineered wild-type dominant-negative overexpression investigated...
Abstract Glioblastoma is the most prevalent and aggressive brain cancer. With a median overall survival of ~15–20 months under standard therapy, novel treatment approaches are desperately needed. A recent phase II clinical trial with personalized immunotherapy based on tumor lysate-charged dendritic cell (DC) vaccination, however, failed to prolong survival. Here, we investigated tissue from patients explore glioblastoma survival-related factors. We followed an innovative approach combining...
Despite the recommendations to initiate β-blockade all patients with an ST-segment elevation myocardial infarction, data concerning timing of administration β-blockers are controversially discussed. In view these controversies, we analyzed effect immediate vs. delayed on all-cause mortality infarction in Lower Austrian Myocardial Infarction Network.Nonrandomized, prospective observational cohort study.Myocardial network including out-of-hospital emergency services, five primary-care...
Surgical training traditionally adheres to the apprenticeship paradigm, potentially exposing trainees an increased risk of complications stemming from their limited experience. To mitigate this risk, augmented and virtual reality have been considered, though effectiveness is difficult assess. The PASSION study seeks investigate improvement manual dexterity following intensive with neurosurgical simulators discern how surgeons´ psychometric characteristics may influence learning process...
There is currently no information available on illness perception in primary cutaneous T-cell lymphomas (CTCL). The aim of this study was therefore to gather initial disease understanding and interpretation patients with CTCL. Consecutive from a hospital-based lymphoma ward completed the Revised Illness Perception Questionnaire (IPQ-R) 2 consecutive visits. A total 24 different variants CTCL were included study. Patients experienced their condition as being long-lasting, but not...
Mast cells (MC) are bone marrow derived haematopoetic playing a crucial role not only in immune response but also the tumor microenvironment with protumorigenic and antitumorigenic functions. The of MC primary cutaneous T-cell lymphomas (CTCL), heterogeneous group non-Hodgkin initial presentation skin, is largely unknown.To gain more accurate information about presence, number, distribution state activation (degranulated vs. non-degranulated) CTCL variants clinical stages.We established...
Resection of high-grade gliomas has been considerably improved by 5-aminolevulinic acid (5-ALA). However, not all neurobiological properties 5-ALA are fully understood. Specifically, potential differences in immune infiltration have conclusively examined, despite recent reports that cells might play a role. Thus, we here provide systematic mapping different fluorescence levels.Tumor-associated macrophages (CD68, CD163), cytotoxic T (CD8), and regulatory (FoxP3) were quantified via three...
Introduction: In this post hoc analysis we compared various response-assessment criteria in newly diagnosed glioblastoma (GB) patients treated with tumor lysate-charged autologous dendritic cells (Audencel) and determined the differences prediction of progression-free survival (PFS) overall (OS). Methods: 76 enrolled a multicenter phase II trial receiving standard care (SOC, n = 40) or SOC + Audencel vaccine (n 36) were included. MRI scans evaluated using MacDonald, RANO, Vol-RANO, mRANO,...
Abstract Patients with high‐grade gliomas are at high risk of venous thromboembolism (VTE). MicroRNAs (miRNAs) small non‐coding RNAs multiple roles in tumour biology, haemostasis and platelet function. Their association VTE glioma has not been comprehensively mapped so far. We thus conducted a nested case–control study within 152 patients WHO grade IV that had part prospective cohort on factors. At inclusion single blood draw was taken, were thereafter followed for maximum 2 years. During...
5-Aminolevulinic acid (5-ALA) is a fluorescent dye that after metabolization to Protoporphyrin IX (PpIX) by the heme biosynthesis pathway typically leads visible fluorescence in WHO grade IV but not II gliomas. The exact mechanism for high PpIX levels gliomas and low fully clarified. To detect relevant changes mRNA expression, we performed an in-silico analysis of glioma sequencing datasets provided Cancer Genome Atlas (TCGA) investigate expression genes: Solute Carrier Family 15 Member 1 2...
The prognosis of diffusely infiltrating glioma patients is dismal but varies greatly between individuals. While characterization gliomas primarily relied on histopathological features, molecular markers increasingly gained importance and play a key role in the recently published 5 th edition World Health Organization (WHO) classification. Heme biosynthesis represents crucial pathway due to its paramount oxygen transport, energy production drug metabolism. Recently, we described "heme mRNA...
In this analysis, we examined the efficacy, feasibility, and limitations of molecular-based targeted therapies in heavily pretreated metastatic colorectal cancer (mCRC) patients after failure all standard treatments. single-center, real-world retrospective analysis our platform for precision medicine, mapped molecular profiles 60 mCRC patients. Tumor samples were analyzed using next-generation sequencing panels mutation hotspots, microsatellite instability testing, immunohistochemistry. All...
Diffusely infiltrating gliomas are characterized by a variable clinical course, and thus novel prognostic biomarkers needed. The heme biosynthesis cycle constitutes fundamental metabolic pathway might play crucial role in glioma biology. aim of this study was to investigate the mRNA expression signature on prognosis large patient cohort. Glioma patients with available sequencing data were retrieved from Cancer Genome Atlas (TCGA). In each patient, calculated categorized into low, medium,...
Abstract BACKGROUND Glioblastoma is the most common brain tumor in adults and with an approximate survival rate of 15 months, new treatment strategies are utmost urgency. In our previous phase II clinical trial, applying immunotherapy dendritic cells to glioblastoma patients, no differences overall were observed. However, distinct miRNAs particular miR-216b, harbored a predictive power associated therapy susceptibility. Thus, we hypothesize that amongst others, miR-216b involved...
Abstract BACKGROUND Glioblastoma is the most aggressive and frequent primary brain tumor. highly immunology-driven because a large portion of its cell mass consists immune cells. In addition to immunology, imaging second important scientific field when it comes glioblastoma. Regarding imaging, VASARI tumor morphology classification system has been gradually accepted as valuable tool. Despite importance both, for glioblastoma characterization, these two disciplines have kept surprisingly...