A5013785090- Fibroblast Growth Factor Research
- Angiogenesis and VEGF in Cancer
- Phagocytosis and Immune Regulation
- CAR-T cell therapy research
- Hearing, Cochlea, Tinnitus, Genetics
- Neuroblastoma Research and Treatments
- Alzheimer's disease research and treatments
- Immune cells in cancer
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- Sarcoma Diagnosis and Treatment
- Cell Image Analysis Techniques
- Zebrafish Biomedical Research Applications
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Protein Degradation and Inhibitors
- Cancer therapeutics and mechanisms
- Chromatin Remodeling and Cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Fetal and Pediatric Neurological Disorders
- Amyotrophic Lateral Sclerosis Research
- Cancer Cells and Metastasis
- Viral Infectious Diseases and Gene Expression in Insects
- Neurological diseases and metabolism
- Cell death mechanisms and regulation
St. Anna Children's Cancer Research Institute
2020-2024
St Anna Children's Hospital
2020-2024
Community College of Rhode Island
2022
Vienna Biocenter
2017-2019
Research Institute of Molecular Pathology
2017-2019
German Center for Neurodegenerative Diseases
2014-2019
Hertie Institute for Clinical Brain Research
2019
Loss of TREM2 function impairs phagocytosis and correlates with decreased soluble in biological fluids patients neurodegenerative disorders.
The most frequent genetic cause of frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS) is the expansion a GGGGCC hexanucleotide repeat in non-coding region chromosome 9 open reading frame 72 (C9orf72) locus. pathological hallmarks observed C9orf72 carriers are formation RNA foci deposition dipeptide (DPR) proteins derived from associated non-ATG (RAN) translation. Currently, it unclear whether foci, DPR translation products, or partial loss predominantly drive...
Abstract Early childhood tumours arise from transformed embryonic cells, which often carry large copy number alterations (CNA). However, it remains unclear how CNAs contribute to tumourigenesis due a lack of suitable models. Here we employ female human stem cell (hESC) differentiation and single-cell transcriptome epigenome analysis assess the effects chromosome 17q/1q gains, are prevalent in embryonal tumour neuroblastoma (NB). We show that impair specification trunk neural crest (NC) cells...
Abstract Neutrophils are evolutionarily conserved innate immune cells playing pivotal roles in host defense. Zebrafish models have contributed substantially to our understanding of neutrophil functions but similarities human maturation not been systematically characterized, which limits their applicability studying disease. Here we show, by generating and analysing transgenic zebrafish strains representing distinct differentiation stages, a high-resolution transcriptional profile maturation....
ABSTRACT Owing to their high efficacy, antimitotic chemotherapeutics are the mainstay for most cancer treatments. However, these drugs do not discriminate between tumor and healthy cells, thus show dose-limiting toxicity severe adverse effects. To improve treatments, rendering tumor-cell specific is highly desirable. Although various strategies, such as targeted antibody–drug conjugates, aim achieve this goal, identification of a tumor-specific ‘Achilles' heel’ remains challenge. Here, we...
Chimeric antigen receptor (CAR) T cells have proven to be a powerful cellular therapy for B cell malignancies. Massive efforts are now being undertaken reproduce the high efficacy of CAR in treatment other Here, predictive preclinical model systems important, and current gold standard evaluation mouse xenografts. However, xenograft assays expensive slow. Therefore, an additional vertebrate vivo assay would beneficial bridge gap from vitro we present novel based on embryonic zebrafish...
Mutations within Leucine-rich repeat kinase 2 (LRRK2) are associated with late-onset Parkinson's disease. The physiological function of LRRK2 and molecular mechanism underlying the pathogenic role mutations remain uncertain. Here, we investigated in intracellular signal transduction. We find that deficiency Lrrk2 rodents affects insulin-dependent translocation glucose transporter type 4 (GLUT4). This deficit is restored during aging by prolonged activation protein B (PKB, Akt) Akt substrate...
Glioblastoma (GBM) is characterized by a particularly invasive phenotype, supported oncogenic signals from the fibroblast growth factor (FGF)/ FGF receptor (FGFR) network. However, possible role of FGFR4 remained elusive so far. Several transcriptomic glioma datasets were analyzed. An extended panel primary surgical specimen-derived and immortalized GBM (stem)cell models original tumor tissues screened for expression. engineered wild-type dominant-negative overexpression investigated...
Ewing sarcoma is a pediatric bone and soft tissue cancer with an urgent need for new therapies to improve disease outcome.To identify effective drugs, phenotypic drug screening has proven be powerful method, but achievable throughput in mouse xenografts, the preclinical standard model, limited.Here, we explored use of xenografts zebrafish high-throughput discover combination sarcoma.We subjected larvae high-content imaging subsequent automated tumor size analysis screen single agents...
Hair cells are specialized sensors located in the inner ear that enable transduction of sound, motion, and gravity into neuronal impulses. In birds some hair contain an iron-rich organelle, cuticulosome, has been implicated magnetic sense. Here, we exploit histological, transcriptomic, tomographic methods to investigate development cuticulosomes, as well molecular subcellular architecture cuticulosome positive cells. We show this organelle forms rapidly after hatching a process involves...
Abstract Early childhood malignancies are driven by sparse genetic aberrations in oncogenes that often co-occur with large copy number variants (CNVs). The combination of these mutations is thought to transform developmentally pliant embryonic cells initiate tumorigenesis. However, the mechanistic interactions between CNVs, oncogenes, and differentiation have not been systematically studied due several obstacles: (i) CNVs cannot be engineered efficiently yet; (ii) transient progenitors...
Overall neuronal outgrowth is not affected. was analyzed in embryos stained with an antibody against acetylated tubulin at 2 dpf. Siblings of GA80-GFPa zebrafish expressing GFP (A) or (B). Scale bar 100 μm. (PDF 6302 kb)
Abstract Early childhood tumours arise from transformed embryonic cells, which often carry large copy number alterations (CNA). However, it remains unclear how CNAs contribute to tumourigenesis due a lack of suitable models. Here we employ female human stem cell (hESC) differentiation and single-cell transcriptome epigenome analysis assess the effects chromosome 17q/1q gains, are prevalent in embryonal tumour neuroblastoma (NB). We show that impair specification trunk neural crest (NC) cells...