A5089229030- Ocular Oncology and Treatments
- Lymphoma Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Cancer Cells and Metastasis
- Advanced Breast Cancer Therapies
- Cancer Research and Treatments
- Cancer Treatment and Pharmacology
- Microtubule and mitosis dynamics
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Chronic Lymphocytic Leukemia Research
- Breast Cancer Treatment Studies
- Immunotherapy and Immune Responses
- CNS Lymphoma Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Viral-associated cancers and disorders
- Estrogen and related hormone effects
- Cancer therapeutics and mechanisms
- Lung Cancer Treatments and Mutations
- Prostate Cancer Treatment and Research
- Retinal Development and Disorders
- HER2/EGFR in Cancer Research
- Neuroendocrine Tumor Research Advances
- RNA modifications and cancer
Institut Curie
2015-2024
Université Paris Sciences et Lettres
2016-2024
Translational Research in Oncology
2008-2023
Université Paris-Saclay
2006-2022
Laboratoire de Recherche en Informatique de Paris 6
2022
Translational Research Institute
2011-2021
Institut Cochin
2015
Assistance Publique – Hôpitaux de Paris
2008-2015
Centre National de la Recherche Scientifique
1998-2012
Inserm
2006-2012
Programmed cell death (PCD) is a physiological process commonly defined by alterations in nuclear morphology (apoptosis) and/or characteristic stepwise degradation of chromosomal DNA occurring before cytolysis. However, determined characteristics PCD such as loss mitochondrial reductase activity or cytolysis can be induced enucleated cells, indicating cytoplasmic control. Here we report sequential disregulation function that precedes shrinkage and fragmentation. A first cyclosporin...
Aggressive T-cell lymphomas (ATCLs) represent 10% to 15% of non-Hodgkin's (NHLs) in adults. ATCLs show a worse prognosis than B-cell lymphomas.On behalf the Société Française de Greffe Moëlle et Thérapie Cellulaire, we conducted retrospective analysis including 77 ATCL patients who underwent allogeneic stem-cell transplantation (alloSCT).The different diagnosis included anaplastic large-cell lymphoma (ALCL; n = 27), peripheral not otherwise specified (PTCL-NOS; angioimmunoblastic (AITL; 11),...
Abstract In a number of experimental systems, the early stage apoptotic process, i.e., that precedes nuclear disintegration, is characterized by breakdown inner mitochondrial transmembrane potential (delta psi(m)). This delta psi(m) disruption may involve permeability transition (PT). Here, we address question whether PT would suffice to cause apoptosis or, rather, it constitute secondary event not causally involved in cascade. Protoporphyrin IX (PPIX), ligand benzodiazepin receptor well...
Abstract The probability that a cell will undergo apoptosis is in part dictated by the cellular redox potential, which mainly determined reduction and oxidation of thiol residues on glutathione proteins. We others have recently shown mitochondria play critical role apoptotic cascade. Here, we address question as to whether modification regulates compartment apoptosis‐regulatory thiols are localized. To resolve this problem, employed divalent thiol‐reactive agent diamide, causes cross‐linking...
The utility of [(18)F]fluorodeoxyglucose (FDG) positron emission tomography-computed tomography (PET-CT) in assessing response at the end induction therapy is well documented Hodgkin's and diffuse large B-cell lymphomas, but its role follicular lymphoma (FL) remains undetermined. We investigated prognostic significance PET-CT performed after first-line patients with FL treated prospective Primary Rituximab Maintenance (PRIMA) study.Results scans immunochemotherapy were recorded...
Breast cancers are composed of molecularly distinct subtypes with different clinical outcomes and responses to therapy. To discover potential therapeutic targets for the poor prognosis-associated triple-negative breast cancer (TNBC), gene expression profiling was carried out on a cohort 130 samples. Polo-like kinase 1 (PLK1) found be significantly overexpressed in TNBC compared other subtypes. High PLK1 confirmed by reverse phase protein tissue microarrays. In cell lines, RNAi-mediated...
Abstract Introduction Identification of new therapeutic agents for breast cancer (BC) requires preclinical models that reproduce the molecular characteristics their respective clinical tumors. In this work, we analyzed genomic and gene expression profiles human BC xenografts corresponding patient Methods Eighteen were obtained by grafting tumor fragments from patients into Swiss nude mice. Molecular characterization tumors was performed DNA copy number analysis using Affymetrix Microarrays....
Uveal melanoma is the most common primary intraocular malignant tumor in adults and defined by a poor natural outcome, as 50% of patients die from metastases. The aim this study was to develop characterize panel human uveal xenografts transplanted into immunodeficient mice.Ninety specimens were grafted severe combined mice, 25 transplantable then established (28%). Relationship between graft clinical, biological, therapeutic features included investigated. Characterization 16 histology,...
CD44 is a marker of tumour-initiating cells and upregulated in invasive breast carcinoma; however, its role the cancer progression unknown. Here, we show that antibody-mediated CD44-targeting human xenografts (HBCx) significantly reduces tumour growth this effect associated to induction growth-inhibiting factors. Moreover, treatment with antibody prevents relapse after chemotherapy-induced remission basal-like HBCx.
Triple-negative breast cancer xenografts with BRCAness, high expression of SLFN11, and RB1 loss are highly sensitive to topoisomerase I inhibitors.
A significant proportion of patients with oestrogen receptor (ER) positive breast cancers (BC) develop resistance to endocrine treatments (ET) and relapse metastatic disease. Here we perform whole exome sequencing gene expression analysis matched primary tumours bone metastasis-derived patient-derived xenografts (PDX). Transcriptomic analyses reveal enrichment the G2/M checkpoint up-regulation Polo-like kinase 1 (PLK1) in PDX. PLK1 inhibition results tumour shrinkage highly proliferating...
Abstract Retinoblastoma is the most frequent intraocular malignancy in children, originating from a maturing cone precursor developing retina. Little known on molecular basis underlying biological and clinical behavior of this cancer. Here, using multi-omics data, we demonstrate existence two retinoblastoma subtypes. Subtype 1, earlier onset, includes heritable forms. It harbors few genetic alterations other than initiating RB1 inactivation corresponds to differentiated tumors expressing...
Abstract The high frequency of homologous recombination deficiency (HRD) is the main rationale testing platinum-based chemotherapy in triple-negative breast cancer (TNBC), however, existing methods to identify HRD are controversial and there a medical need for predictive biomarkers. We assess vivo response platinum agents 55 patient-derived xenografts (PDX) TNBC determinants response. status, determined from whole genome sequencing, highly BRCA1 promoter methylation not associated with...
Early apoptosis is invariably accompanied by a disruption of inner mitochondrial transmembrane potential (ΔΨm). Cationic lipophilic fluorochromes, such as 3,3′ dihexyloxacarbocyanine iodide (DiOC6(3)), rhodamine 123, or 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine (JC-1), can be used to measure an apoptotic ΔΨm dissipation. However, these dyes are afflicted the handicap that cytofluorometric analyses must performed ad hoc on nonfixed, metabolically active cells. Here,...
Resistance to apoptosis is a frequent characteristic of cancer cells and participates both in the initial phase carcinogenesis development chemotherapy resistance. Recently, it has become clear that disruption mitochondrial membrane function decisive event apoptotic process leading disposal chemotherapy-treated cells. Opening megachannel (also called permeability transition pore) at least part responsible for integrity apoptosis. The regulated by numerous endogenous effectors including...