A5004080949- Cell death mechanisms and regulation
- HIV Research and Treatment
- Natural product bioactivities and synthesis
- Ion Channels and Receptors
- Bioactive Compounds and Antitumor Agents
- Mitochondrial Function and Pathology
- Bioactive natural compounds
- Immune cells in cancer
- Sesquiterpenes and Asteraceae Studies
- Neuroscience and Neuropharmacology Research
- Essential Oils and Antimicrobial Activity
- Piperaceae Chemical and Biological Studies
- Asthma and respiratory diseases
- Drug Transport and Resistance Mechanisms
- Cannabis and Cannabinoid Research
- Synthesis and Biological Activity
- Genomics, phytochemicals, and oxidative stress
- Allergic Rhinitis and Sensitization
- HIV/AIDS drug development and treatment
- HIV-related health complications and treatments
- Olfactory and Sensory Function Studies
- Plant chemical constituents analysis
- Bioactive Natural Diterpenoids Research
- Bee Products Chemical Analysis
- Sulfur Compounds in Biology
Universidade Federal de Mato Grosso
2016-2023
Universidade de Brasília
2019-2023
University of Córdoba
1999-2014
University of Naples Federico II
2001-2005
University of Siena
2004
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2004
Universidad de Salamanca
2003
Hospital Universitario Reina Sofía
1998-2002
Menéndez Pelayo International University
2002
University of Molise
2001
Programmed cell death (PCD) is a physiological process commonly defined by alterations in nuclear morphology (apoptosis) and/or characteristic stepwise degradation of chromosomal DNA occurring before cytolysis. However, determined characteristics PCD such as loss mitochondrial reductase activity or cytolysis can be induced enucleated cells, indicating cytoplasmic control. Here we report sequential disregulation function that precedes shrinkage and fragmentation. A first cyclosporin...
Bcl-2 belongs to a family of apoptosis-regulatory proteins which incorporate into the outer mitochondrial as well nuclear membranes. The mechanism by proto-oncogene product inhibits apoptosis is thus far elusive. We and others have shown previously that first biochemical alteration detectable in cells undergoing apoptosis, before changes become manifest, collapse inner membrane potential (delta psi m), suggesting involvement products apoptotic cascade. Here we show mitochondria contain...
In a number of experimental systems, the early stage apoptotic process, i.e., that precedes nuclear disintegration, is characterized by breakdown inner mitochondrial transmembrane potential (delta psi m). This delta m disruption mediated opening permeability transition (PT) pores and appears to be critical for cascade, since it directly regulated Bcl-2 mitochondria induced undergo PT in vitro become capable inducing chromatinolysis cell-free system apoptosis. Here, we addressed question...
When cells undergo nuclear apoptosis (chromatin condensation, DNA fragmentation), they already manifest at least three alterations that can be quantified cytofluorometrically the single-cell level: 1) a loss of mitochondrial transmembrane potential (delta psi m), 2) an increased production superoxide anions, and 3) aberrant exposure phosphatidylserine (PS) residues on outer plasma membrane leaflet. This latter alteration allows for phagocytic recognition/elimination apoptotic cells. In this...
Abstract According to current understanding, several metabolic alterations form part of the common phase apoptosis process. Such include a disruption mitochondrial transmembrane potential (delta psi(m)), depletion nonoxidized glutathione (GSH) levels, an increase in production reactive oxygen species (ROS), and elevation cytosolic free Ca2+ levels. Using cytofluorometric approach, we have determined each these parameters at single cell level thymocytes or T hybridoma cells undergoing...
Abstract The probability that a cell will undergo apoptosis is in part dictated by the cellular redox potential, which mainly determined reduction and oxidation of thiol residues on glutathione proteins. We others have recently shown mitochondria play critical role apoptotic cascade. Here, we address question as to whether modification regulates compartment apoptosis‐regulatory thiols are localized. To resolve this problem, employed divalent thiol‐reactive agent diamide, causes cross‐linking...
Early apoptosis is invariably accompanied by a disruption of inner mitochondrial transmembrane potential (ΔΨm). Cationic lipophilic fluorochromes, such as 3,3′ dihexyloxacarbocyanine iodide (DiOC6(3)), rhodamine 123, or 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine (JC-1), can be used to measure an apoptotic ΔΨm dissipation. However, these dyes are afflicted the handicap that cytofluorometric analyses must performed ad hoc on nonfixed, metabolically active cells. Here,...
Caffeic acid phenethyl ester (CAPE), which is derived from the propolis of honeybee hives, has been shown to reveal anti-inflammatory properties. Since T-cells play a key role in onset several inflammatory diseases, we have evaluated immunosuppressive activity CAPE human T-cells, discovering that this phenolic compound potent inhibitor early and late events T-cell receptor-mediated activation. Moreover, found specifically inhibited both interleukin (IL)-2 gene transcription IL-2 synthesis...
Abstract We have recently shown that lymphocyte apoptosis induced by dexamethasone or superantigens is accompanied reduction of mitochondrial transmembrane potential (ΔΨ m ) which precedes nuclear DNA fragmentation. Here, we demonstrate fluorochromes such as 3,3′dihexyloxacarbocyanine iodide [DiOC 6 (3)] measure ΔΨ , hydroethidine (HE) superoxide anion production allow the identification thymocytes peripheral T lymphocytes are eliminated in vivo . In mice bearing transgenic α/β cell receptor...
Capsiate and its dihydroderivatives are the major capsaicinoids of sweet pepper. These new do not activate vanilloid receptor type 1 (VR1) but they share with capsaicin (CPS)some biological activities mediated in a VR1-independent fashion. In this study we show that CPS nordihydrocapsiate (CPT) inhibit early late events T cell activation, including CD69, CD25 ICAM-1 surface expression, progression to S phase cycle proliferation response TCR CD28 co-engagement. Moreover, both CPT NF-κB...
The induction of cell death in leukemic HL-60 cells by the ether lipid 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine (ET-18-OCH3; edelfosine) followed typical apoptotic changes ultrastructural morphology, including blebbing, chromatin condensation, nuclear membrane breakdown and extensive vacuolation. Using a cytofluorimetric approach, we found that ET-18-OCH3 induced disruption mitochondrial transmembrane potential (ΔΨm) production reactive oxygen species (ROS) DNA fragmentation...
Pharmacogenomics (PGx) is considered an emergent field in developing countries. Research on PGx the Latin American and Caribbean (LAC) region remains scarce, with limited information some populations. Thus, extrapolations are complicated, especially mixed In this paper, we reviewed analyzed pharmacogenomic knowledge among LAC scientific clinical community examined barriers to application. We performed a search for publications trials worldwide evaluated contribution of LAC. Next, conducted...
Investigation of polar extracts from ripe fruits Capsicum annuum L. var. acuminatum yielded three new glycosides, capsosides A (1) and B (2) capsianoside VII (3), along with seven known compounds (4−10). The chemical structures were elucidated mainly by extensive nuclear magnetic resonance methods mass spectrometry, the biological activities icariside E5 (4) tested different assays. Icariside E5, in contrast to capsaicin, neither induces an increase intracellular levels reactive oxygen...
Abstract Endogenous N-acyl dopamines such as N-arachidonoyldopamine (NADA) and N-oleoyldopamine have been recently identified a new class of brain neurotransmitters sharing endocannabinoid endovanilloid biological activities. As endocannabinoids show immunomodulatory activity, T cells play key role in the onset several diseases that affect CNS, we evaluated immunosuppressive activity NADA human cells, discovering both compounds are potent inhibitors early late events TCR-mediated cell...
Opopanax chironium is a rich source of furano- and dihydrofuranocoumarins, whose accumulation in all plant parts especially the roots presumably responsible for poisonous properties species. The presence two distinct chemotypes was evidenced, with one from Sicily affording new dihydrofuranocoumarins 5d 5e, while extracts Sardinian chemotype showed powerful apoptotic activity, which traced to prenylated furanocoumarins heraclenin (2a) imperatorin (2b). Despite close structural similarity,...
The ascidian Aplidium conicum collected along Sardinia coasts (Italy) contained two novel prenylated benzoquinones, designated thiaplidiaquinone A (1) and B (2). These compounds showed an unprecedented tetracyclic structure. We have studied the pro-apototic mechanisms of both benzoquinones in Jurkat cell line that is derived from a human T lymphoma, we show induce strong production intracellular reactive oxygen species (ROS) this line. Moreover, kinetic experiments, comparing timing ROS...