A5068539997- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- Proteoglycans and glycosaminoglycans research
- Cancer, Hypoxia, and Metabolism
- Chronic Myeloid Leukemia Treatments
- Melanoma and MAPK Pathways
- Glycosylation and Glycoproteins Research
- ATP Synthase and ATPases Research
- Sperm and Testicular Function
- Nerve injury and regeneration
- Reproductive Biology and Fertility
- Immune Response and Inflammation
- Fibroblast Growth Factor Research
- Synthesis and Characterization of Pyrroles
- Immunotherapy and Immune Responses
- Marine Sponges and Natural Products
- RNA Interference and Gene Delivery
- HER2/EGFR in Cancer Research
- Neuroscience and Neuropharmacology Research
- Brain Metastases and Treatment
- Cardiac Ischemia and Reperfusion
- Retinoids in leukemia and cellular processes
- Reconstructive Surgery and Microvascular Techniques
- Acute Myeloid Leukemia Research
- Photodynamic Therapy Research Studies
Inserm
2015-2024
Université de Lille
2015-2024
Centre National de la Recherche Scientifique
1995-2024
Centre Hospitalier Universitaire de Lille
2011-2024
Banque Populaire
2011-2024
Onco Lille
2016-2024
CANTHER - Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers
2020-2024
Institut pour la Recherche sur le Cancer de Lille
2007-2023
Hôpital Roger Salengro
2022
European Molecular Biology Laboratory
2021
Programmed cell death (PCD) is a physiological process commonly defined by alterations in nuclear morphology (apoptosis) and/or characteristic stepwise degradation of chromosomal DNA occurring before cytolysis. However, determined characteristics PCD such as loss mitochondrial reductase activity or cytolysis can be induced enucleated cells, indicating cytoplasmic control. Here we report sequential disregulation function that precedes shrinkage and fragmentation. A first cyclosporin...
Anucleate cells can be induced to undergo programmed cell death (PCD), indicating the existence of a cytoplasmic PCD pathway that functions independently from nucleus. Cytoplasmic structures including mitochondria have been shown participate in control apoptotic nuclear disintegration. Before exhibit common signs apoptosis (chromatin condensation and endonuclease-mediated DNA fragmentation), they reduction mitochondrial transmembrane potential (delta psi m) may due opening permeability...
In a number of experimental systems in which lymphocyte depletion was induced by apoptosis-inducing manipulations, no apoptotic morphology and ladder-type DNA fragmentation were detected among freshly isolated peripheral lymphocytes ex vivo. Here we report that one alteration can be splenocytes stimulated with lymphocyte-depleting doses dexamethasone (DEX) vivo is reduced uptake 3,3'dihexyloxacarbocyanine iodide (DiOC6[3]), fluorochrome incorporates into cells dependent upon their...
Bcl-2 belongs to a family of apoptosis-regulatory proteins which incorporate into the outer mitochondrial as well nuclear membranes. The mechanism by proto-oncogene product inhibits apoptosis is thus far elusive. We and others have shown previously that first biochemical alteration detectable in cells undergoing apoptosis, before changes become manifest, collapse inner membrane potential (delta psi m), suggesting involvement products apoptotic cascade. Here we show mitochondria contain...
In a number of experimental systems, the early stage apoptotic process, i.e., that precedes nuclear disintegration, is characterized by breakdown inner mitochondrial transmembrane potential (delta psi m). This delta m disruption mediated opening permeability transition (PT) pores and appears to be critical for cascade, since it directly regulated Bcl-2 mitochondria induced undergo PT in vitro become capable inducing chromatinolysis cell-free system apoptosis. Here, we addressed question...
Aims: The pathophysiological role of iron in Parkinson's disease (PD) was assessed by a chelation strategy aimed at reducing oxidative damage associated with regional deposition without affecting circulating metals. Translational cell and animal models provided concept proofs delayed-start (DS) treatment paradigm, the basis for preliminary clinical assessments. Results: For translational studies, we effect insults mice systemically prechelated deferiprone (DFP) following motor functions,...
In a number of experimental systems, the early stage apoptotic process, i.e. which precedes nuclear disintegration, is characterized by breakdown inner mitochondrial transmembrane potential ( ΔΨ m ). Here we address question as to whether permeability transition (PT) pores may account for dissipation in lymphocyte apoptosis. Drugs known their PT‐inhibitory (bongkrekic acid, cyclosporin A, and non‐immunosuppressive A analogue N ‐methyl‐Val‐4‐cyclosporin A) are capable preventing disruption....
Bile acids are signalling molecules, which activate the transmembrane receptor TGR5 and nuclear FXR. BA sequestrants (BAS) complex bile in intestinal lumen decrease FXR activity. The BAS–BA also induces glucagon-like peptide-1 (GLP-1) production by L cells potentiates β-cell glucose-induced insulin secretion. Whether is expressed controls GLP-1 unknown. Here, we show that activation decreases proglucagon expression interfering with glucose-responsive factor Carbohydrate-Responsive Element...
BACKGROUND: Sperm cell death appears to be a cause of male infertility. The objective this study was determine the most reliable method for evaluation sperm quality in semen samples during preparation IVF. METHODS: Conventional analysis compared with several cytofluorometric methods detecting death-associated changes. Neat from infertile patients and prepared by PureSperm® gradient were studied conventional microscopy analysed mitochondrial membrane potential (ΔΨΨm), generation reactive...
When cells undergo nuclear apoptosis (chromatin condensation, DNA fragmentation), they already manifest at least three alterations that can be quantified cytofluorometrically the single-cell level: 1) a loss of mitochondrial transmembrane potential (delta psi m), 2) an increased production superoxide anions, and 3) aberrant exposure phosphatidylserine (PS) residues on outer plasma membrane leaflet. This latter alteration allows for phagocytic recognition/elimination apoptotic cells. In this...
Endotoxin is one of the major factors causing myocardial depression and death during sepsis in humans. Recently, it was reported that endotoxin may induce cardiomyocyte apoptosis. Also, multiple caspase activation has been implicated endotoxin-induced apoptosis several organ systems. In this study, we investigated whether would increase activities evaluated effects vivo administration (3 mg/kg) broad-spectrum inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone(z-VAD.fmk),...
Abstract According to current understanding, several metabolic alterations form part of the common phase apoptosis process. Such include a disruption mitochondrial transmembrane potential (delta psi(m)), depletion nonoxidized glutathione (GSH) levels, an increase in production reactive oxygen species (ROS), and elevation cytosolic free Ca2+ levels. Using cytofluorometric approach, we have determined each these parameters at single cell level thymocytes or T hybridoma cells undergoing...
Abstract In a number of experimental systems, the early stage apoptotic process, i.e., that precedes nuclear disintegration, is characterized by breakdown inner mitochondrial transmembrane potential (delta psi(m)). This delta psi(m) disruption may involve permeability transition (PT). Here, we address question whether PT would suffice to cause apoptosis or, rather, it constitute secondary event not causally involved in cascade. Protoporphyrin IX (PPIX), ligand benzodiazepin receptor well...
Abstract The probability that a cell will undergo apoptosis is in part dictated by the cellular redox potential, which mainly determined reduction and oxidation of thiol residues on glutathione proteins. We others have recently shown mitochondria play critical role apoptotic cascade. Here, we address question as to whether modification regulates compartment apoptosis‐regulatory thiols are localized. To resolve this problem, employed divalent thiol‐reactive agent diamide, causes cross‐linking...
Sperm motility evaluation is associated with fertility in IVF programmes. The visual estimation of sperm extremely subjective. Hence, alternative methods are required. Among them, determination mitochondrial membrane potential (Deltapsi(m)) changes spermatozoa using potentiometric dyes may be a reliable test to determine quality. However, the use samples has not been compared.We have studied from 28 infertile patients enrolled an programme flow cytometry after staining four commonly used...
Abstract Heparanase (HPSE) is a potent protumorigenic, proangiogenic, and prometastatic enzyme that overexpressed in brain metastatic breast cancer (BMBC). However, little known about the regulation of this potential therapeutic target BMBC, which remains very poorly managed clinic. We hypothesized HPSE gene expression might be regulated by micro RNA exploited therapeutically. Using miRanda RNAhybrid, we identified miR-1258 as candidate may directly suppress BMBC. In support our hypothesis,...