A5018287918- Ocular Oncology and Treatments
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Colorectal and Anal Carcinomas
- Intraperitoneal and Appendiceal Malignancies
- Nanoplatforms for cancer theranostics
- Colorectal Cancer Surgical Treatments
- Cancer Cells and Metastasis
- Gastric Cancer Management and Outcomes
- Pancreatic and Hepatic Oncology Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Enhanced Recovery After Surgery
- Appendicitis Diagnosis and Management
- Ovarian cancer diagnosis and treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Anorectal Disease Treatments and Outcomes
- Retinal Development and Disorders
- Cardiac, Anesthesia and Surgical Outcomes
- Cutaneous Melanoma Detection and Management
- Cancer Research and Treatments
Institut Curie
2015-2024
Université Paris Sciences et Lettres
2016-2024
Groupe Francophone des Myélodysplasies
2021-2024
Université Paris Cité
2012-2022
Assistance Publique – Hôpitaux de Paris
2009-2020
Hôpital Lyon Sud
2013-2018
Lyon College
2018
Hôpital Saint-Antoine
1993-2015
Sorbonne Université
1993-2015
Hôpital Robert-Debré
2015
Abstract Purpose: Patient-derived xenograft models are considered to represent the heterogeneity of human cancers and advanced preclinical models. Our consortium joins efforts extensively develop characterize a new collection patient-derived colorectal cancer (CRC) Experimental Design: From 85 unsupervised surgical samples collection, 54 tumors were successfully xenografted in immunodeficient mice rats, representing 35 primary tumors, 5 peritoneal carcinoses 14 metastases. Histologic...
At the stage of carcinoma in situ, basement membrane (BM) segregates tumor cells from stroma. This barrier must be breached to allow dissemination adjacent tissues. Cancer can perforate BM using proteolysis; however, whether stromal play a role this process remains unknown. Here we show that an abundant cell population, cancer-associated fibroblasts (CAFs), promote cancer invasion through BM. CAFs facilitate breaching matrix metalloproteinase-independent manner. Instead, pull, stretch, and...
Cancer-associated fibroblasts (CAFs) are the most abundant cells of tumor stroma. Their capacity to contract matrix and induce invasion cancer has been well documented. However, it is not clear whether CAFs remodel by other means, such as degradation, deposition, or stiffening. We now show that assemble fibronectin (FN) trigger mainly via integrin-αvβ3. In absence FN, contractility preserved, but their ability abrogated. When degradation impaired, retain in an FN-dependent manner. The level...
Abstract During tumor progression, cancer-associated fibroblasts (CAFs) accumulate in tumors and produce an excessive extracellular matrix (ECM), forming a capsule that enwraps cancer cells. This acts as barrier restricts growth leading to the buildup of intratumoral pressure. Combining genetic physical manipulations vivo with microfabrication force measurements vitro, we found CAFs is not passive but instead actively compresses cells using actomyosin contractility. Abrogation contractility...
Circulating tumor cells (CTCs) and circulating DNA (ctDNA) have been recently investigated in several cancer types, but their respective clinical significance remains to be determined. In our prospective study, we compared the detection rate prognostic value of these two biomarkers patients with metastatic uveal melanoma. GNAQ/GNA11 mutations were characterized archived tissue. Using a highly sensitive mutation‐specific bidirectional pyrophosphorolysis‐activated polymerization (bi‐PAP)...
Uveal melanoma is the most common primary intraocular malignant tumor in adults and defined by a poor natural outcome, as 50% of patients die from metastases. The aim this study was to develop characterize panel human uveal xenografts transplanted into immunodeficient mice.Ninety specimens were grafted severe combined mice, 25 transplantable then established (28%). Relationship between graft clinical, biological, therapeutic features included investigated. Characterization 16 histology,...
Metastatic uveal melanoma is a deadly disease with no proven standard of care. Here we present metastatic patient an exceptional high sensitivity to PD-1 inhibitor associated outlier CpG>TpG mutation burden, MBD4 germline deleterious mutation, and somatic inactivation in the tumor. We identify additional tumors The Cancer Genome Atlas (TCGA) cohorts similar hypermutator profiles patients carrying mutations loss heterozygosity. This MBD4-related phenotype may explain unexpected responses...
The management of patients with colorectal cancer (CRC) and potentially resectable liver metastases (LM) requires quick assessment mutational status response to pre-operative systemic therapy. In a prospective phase II trial (NCT01442935), we investigated the clinical validity circulating tumor cell (CTC) DNA (ctDNA) detection. CRC LM were treated first-line triplet or doublet chemotherapy combined targeted CTC (Cellsearch®) Kirsten RAt Sarcoma (KRAS) ctDNA (droplet digital polymerase chain...
Disruption of splicing patterns due to mutations genes coding factors in tumors represents a potential source tumor neoantigens, which would be both public (shared between patients) and tumor-specific (not expressed normal tissues). In this study, we show that the factor SF3B1 uveal melanoma generate such immunogenic neoantigens. Memory CD8+ T cells specific for these neoantigens are preferentially found 20% patients with bearing SF3B1-mutated tumors. Single-cell analyses neoepitope-specific...
Incurable metastases develop in approximately 50% of patients with uveal melanoma (UM). The purpose this study was to analyze genomic profiles a large series ocular tumors and liver design genome-based classifier for metastatic risk assessment.A 86 UM 66 were analyzed by using BAC CGH (comparative hybridization) microarray. A clustering performed, correlation the status sought among subset 71 minimum follow-up 24 months. chromosome 3 further examined tumors, disomy checked an SNP prognostic...
Abstract Purpose: Chemoradiotherapy (CRT) is the current standard of care for patients diagnosed with locally advanced anal squamous cell carcinoma (ASCC), but some develop local and/or distant relapse during follow-up. This study was designed to monitor human papillomavirus (HPV) circulating tumor DNA (ctDNA) levels CRT in ASCC. Experimental Design: We analyzed samples from HPV16- or HPV18-positive Blood were collected before and after CRT. HPV16 HPV18 ctDNA detection performed by droplet...
Abstract Purpose: To develop a molecular tool to detect circulating tumor–derived DNA (ctDNA) in the plasma from patients with uveal melanoma as marker of tumor burden and monitor treatment efficacy. Experimental Design: A real-time PCR was developed on basis bidirectional pyrophosphorolysis-activated polymerization (bi-PAP) for quantification ctDNA using 3′blocked primer pairs specific 3 recurrent mutually exclusive mutations Gα subunits GNAQ GNA11. Results: Sensitivity specificity bi-PAP...
Up to 50% of uveal melanomas (UM) metastasise the liver within 10 years diagnosis, and these almost always prove rapidly fatal. As histopathological growth patterns (HGPs) metastases replacement desmoplastic type, particularly from colon breast carcinoma, may import valuable biological prognostic information, we have studied HGP in a series 41 UM originating patients period 2006-2017. Twenty underwent enucleation while 21 had radiation therapy. Analysis by array comparative genomic...