A5071294409- Colorectal Cancer Treatments and Studies
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- Genetic factors in colorectal cancer
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Ovarian cancer diagnosis and treatment
- PARP inhibition in cancer therapy
- Pancreatic and Hepatic Oncology Research
- Cell death mechanisms and regulation
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- RNA Research and Splicing
- Circular RNAs in diseases
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Microtubule and mitosis dynamics
- Advanced Breast Cancer Therapies
- Liver physiology and pathology
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Metal complexes synthesis and properties
- Molecular Biology Techniques and Applications
Université de Caen Normandie
2018-2025
Inserm
2010-2025
Normandie Université
2018-2025
Centre François Baclesse
2018-2025
Cancers et Préventions
2018-2025
Unité de recherche interdisciplinaire pour la prévention et le traitement des cancers
2017-2024
UniCancer Group
2023
Centre François Baclesse
2020
University of British Columbia
2015-2017
Canada's Michael Smith Genome Sciences Centre
2017
Abstract Purpose: Patient-derived xenograft models are considered to represent the heterogeneity of human cancers and advanced preclinical models. Our consortium joins efforts extensively develop characterize a new collection patient-derived colorectal cancer (CRC) Experimental Design: From 85 unsupervised surgical samples collection, 54 tumors were successfully xenografted in immunodeficient mice rats, representing 35 primary tumors, 5 peritoneal carcinoses 14 metastases. Histologic...
Abstract Obtaining representative human colon cancer cell lines from fresh tumors is technically difficult. Using 32 tumor fragments patients with cancer, the present study shows that prior xenograft leads to more efficient line establishment compared direct (P < 0.05). From 26 specimens, we successfully established 20 xenografts in nude mice (77%); among 19 of these xenografts, 9 (47%) led lines, including four liver metastases. Only 3 31 specimens (9.7%) grew immediately vitro, and...
// Elham Hosseini-Beheshti 1, 3 , Wendy Choi Louis-Bastien Weiswald 4 Geetanjali Kharmate Mazyar Ghaffari Mani Roshan-Moniri Mohamed D. Hassona Leslie Chan Mei Yieng Chin Isabella T. Tai Paul S. Rennie 2, Ladan Fazli Emma Tomlinson Guns 1 Department of Experimental Medicine University British Columbia, Vancouver, V6H 3Z6, Canada 2 Urologic Sciences The Vancouver Prostate Centre Division Gastroenterology, Correspondence to: Guns, e-mail: eguns@prostatecentre.com Keywords: exosomes, cancer...
Development of targeted therapeutics required translationally relevant preclinical models with well-characterized cancer genome alterations. Here, by studying 52 colorectal patient-derived tumor xenografts (PDX), we examined key molecular alterations the IGF2-PI3K and ERBB-RAS pathways response to cetuximab. PDX data were compared that published for patient tumors in The Cancer Genome Atlas. We demonstrated a significant pattern mutual exclusivity genomic abnormalities pathways. anomaly...
Abstract Background Multicellular tumour sphere models have been shown to closely mimic phenotype characteristics of in vivo solid tumours, or allow vitro propagation cancer stem cells (CSCs). CSCs are usually characterized by the expression specific membrane markers using flow cytometry (FC) after enzymatic dissociation. Consequently, spatial location positive within spheres is not documented. Confocal microscopy best technique for imaging thick biological specimens multi-labelling but...
New models continue to be required improve our understanding of colorectal cancer progression. To this aim, we characterised in study a three-dimensional multicellular tumour model that named colospheres, directly obtained from mechanically dissociated colonic primary tumours and correlated with metastatic potential.Colorectal (n=203) 120 paired non-tumoral colon mucosa were disaggregated into small fragments for short-term cultures. Features producing colospheres analysed. Further...
Ovarian cancer (OC) is the leading cause of death in patients with gynecologic cancers. Due to late diagnosis and resistance chemotherapy, 5-year survival rate OC below 40%. We observed that UCA1, a lncRNA previously reported play an oncogenic role several malignancies, overexpressed chemoresistant cell line OAW42-R compared their chemotherapy-sensitive counterpart OAW42. Additionally, UCA1 overexpression was related poor prognosis two independent patient cohorts. Currently, molecular...
Abstract Background Radiotherapy is one of the cornerstones treatment Head and Neck Squamous Cell Carcinomas ( HNSCC). However, radioresistance associated with a high risk recurrence. To propose strategies (such as combinations drugs) that could over intrinsic radioresistance, it crucial to predict response treatment. Patient-Derived Tumor Organoids (PDTO) are in vitro tridimensional microtumors obtained from patient’ own cancer samples. They have been shown serve reliable surrogates tumor...
There is an urgent need of precision medicine for ovarian cancer patients to identify who respond chemotherapy and PARP inhibitors, a therapy targeting homologous recombination deficiency (HRD). Here we established panel 37 long-term patient-derived tumor organoids (PDTO) models various histological subtypes from 224 demonstrated that they mimic the molecular characteristics original tumors. Screening chemotherapeutic drugs showed PDTO exhibit heterogeneous responses, response high-grade...
To determine the role of Wnt antagonist Dickkopf (DKK) 1 in human endothelial colony-forming cells (ECFCs) view emerging importance pathways vascular biology.Endothelial progenitor have been proposed to be crucial tumor neovascularization. Recombinant DKK1 has tested ECFC angiogenic properties vitro. enhanced proliferation and capacity ECFCs form pseudotubes Matrigel. These effects attributed enhancement growth factor receptor 2, SDF-1, CXCR4. gene silencing realized on mesenchymal stem...
Ex vivo colospheres have been previously characterised as a colorectal cancer (CRC) well-rounded multicellular model, exclusively formed by carcinoma cells, and derived from fresh CRC tissue after mechanical dissociation. The ability to form was correlated with tumour aggressiveness. Their three-dimensional conformation prompted us further investigate their potential interest preclinical tool. Patient-derived xenografts were used produce numerous colospheres. Mechanism of formation...
Abstract Background Perioperative chemotherapy and surgery are a standard of care for patients with resectable gastric or gastroesophageal junction (GEJ) adenocarcinoma. However, the prognosis remains poor this population. The FLOT (fluorouracil, leucovorin, oxaliplatin, docetaxel) regimen is considered as new perioperative strategy, despite associated 5-year overall survival rate (OS) amounting 45% following radical surgery. Immunotherapy antibodies that inhibit PD-1/ PD-L1 interaction has...
Abstract Background In the era of personalized medicine, establishment preclinical models cancer that faithfully recapitulate original tumors is essential to potentially guide clinical decisions. Methods We established 7 [4 cell lines, 2 Patient-Derived Tumor Organoids (PDTO) and 1 Xenograft (PDX)], all derived from same Ovarian Clear Cell Carcinoma (OCCC). To determine relevance each these models, comprehensive characterization was performed based on morphological, histological,...
Abstract Novel therapeutic strategies are urgently required for the clinical management of chemoresistant ovarian carcinoma, which is most lethal gynecologic malignancies. miRNAs hold promise because they play a critical role in determining cell phenotype by regulating several hundreds targets, could constitute vulnerabilities cancer cells. A combination gain-of-function miRNA screening and real-time continuous monitoring allows identification with robust cytotoxic effects Focusing on...
Abstract Ovarian cancer represents the first cause of mortality from gynecologic malignancies due to frequent chemoresistance occurrence. Increasing [BH3-only Bim, Puma, Noxa proapoptotic]/[Bcl-x L , Mcl-1 antiapoptotic] proteins ratio was proven efficiently kill ovarian carcinoma cells and development new molecules imbalance Bcl-2 member equilibrium are strongly required. Drug repurposing constitutes an innovative approach rapidly develop therapeutic strategies through exploitation...
Although improvements have been made in the management of pancreatic adenocarcinoma (PDAC) during past 20 years, prognosis this deadly disease remains poor with an overall 5-year survival under 10%. Treatment FOLFIRINOX, a combined regimen 5-fluorouracil, irinotecan (SN-38) and oxaliplatin, is nonetheless associated excellent initial tumour response its use has allowed numerous patients to go through surgery while their was initially considered unresectable. These discrepancies between very...