A5005602008- Chronic Lymphocytic Leukemia Research
- Epigenetics and DNA Methylation
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Cancer-related molecular mechanisms research
- Neuroblastoma Research and Treatments
- Pluripotent Stem Cells Research
- Birth, Development, and Health
- Genomics and Chromatin Dynamics
- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- Neuroendocrine regulation and behavior
- Adipose Tissue and Metabolism
- Chromosomal and Genetic Variations
- Nuclear Receptors and Signaling
- Circular RNAs in diseases
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Lipid metabolism and biosynthesis
- interferon and immune responses
- Pancreatic function and diabetes
- RNA regulation and disease
- Chromatin Remodeling and Cancer
- Genomics and Phylogenetic Studies
- Maternal Mental Health During Pregnancy and Postpartum
St. Anna Children's Cancer Research Institute
2023-2024
St Anna Children's Hospital
2023-2024
University of Regensburg
2020-2024
University of Gothenburg
2018-2019
Abstract Early childhood tumours arise from transformed embryonic cells, which often carry large copy number alterations (CNA). However, it remains unclear how CNAs contribute to tumourigenesis due a lack of suitable models. Here we employ female human stem cell (hESC) differentiation and single-cell transcriptome epigenome analysis assess the effects chromosome 17q/1q gains, are prevalent in embryonal tumour neuroblastoma (NB). We show that impair specification trunk neural crest (NC) cells...
Nonalcoholic fatty liver disease (NAFLD) is currently the most prevalent form of worldwide. This term encompasses a spectrum pathologies, from benign hepatic steatosis to non-alcoholic steatohepatitis, which have, date, been challenging model in laboratory setting. Here, we present human pluripotent stem cell (hPSC)-derived steatosis, overcomes inherent challenges current models and provides insights into metabolic rewiring associated with steatosis. Following induction macrovesicular...
Chronic lymphocytic leukemia (CLL) has been a good model system to understand the functional role of 5-methylcytosine (5-mC) in cancer progression. More recently, an oxidized form 5-mC, 5-hydroxymethylcytosine (5-hmC) gained lot attention as regulatory epigenetic modification with prognostic and diagnostic implications for several cancers. However, there is no global study exploring levels CLL. Herein, using mass spectrometry hMeDIP-sequencing, we analysed dynamics 5-hmC during B cell...
Abstract Exposure to early life stress (ELS) during childhood or prenatally increases the risk of future psychiatric disorders. The effect exposure neonatal period is less well understood. In preterm infants, invasive procedures associated with altered brain development and responses suggesting that could be a key time for programming mental health. Previous studies suggest ELS affects hypothalamic epigenome, making it good candidate mediate these effects. this study, we used mouse model...
Micrococcal nuclease sequencing is the state-of-the-art method for determining chromatin structure and nucleosome positioning. Data analysis complex due to AT-dependent sequence bias of endonuclease requirement high depth. Here, we present nucleosome-based MNase accessibility (nucMACC) pipeline unveiling regulatory landscape by measuring stability. The nucMACC represents a systematic genome-wide approach detecting unstable ("fragile") nucleosomes. We have characterized in
Long non-coding RNAs (lncRNAs) have been shown to modulate gene expression and are involved in the initiation progression of various cancer types. Despite wealth studies describing transcriptome changes upon lncRNA knockdown, there is limited information lncRNA-mediated effects on regulatory elements (REs) modulating expression. In this study, we investigated how metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) regulates primary target genes using time-resolved MALAT1...
Abstract Early life stress during childhood is associated with a number of psychiatric disorders that manifest across the course. Preterm birth profound stressor, and an important cause cognitive impairment, as well neurodevelopmental disorders. However, mechanisms link events early neonatal period later functional problems are poorly understood. We developed novel mouse model (modified maternal separation; MMS) specific relevance to preterm (PTB) hypothesised it would affect hypothalamic...
Summary Chromatin-associated long non-coding RNAs (lncRNAs) have been shown to define chromatin density, regulate gene expression, and are involved in the initiation progression of various cancer types. Despite wealth studies describing transcriptome changes upon lncRNA modulation, little data is showing direct effects on regulatory elements (REs) that drive expression. Here we explored molecular mechanism chromatin-interacting lncRNA, MALAT1, through RNA- ATAC-seq, using HeLa cells as a...
Summary Nonalcoholic fatty liver disease (NAFLD) affects ~88% of obese individuals and is characterised by hepatic lipid accumulation. Mitochondrial metabolic dysfunction a feature NAFLD. We used human pluripotent stem cell-based system to determine how mitochondrial linked induced accumulation in hepatocyte-like cells (HLCs) using lactate, pyruvate octanoate (LPO). Transcriptomic analysis revealed perturbation respiratory pathways LPO exposed cells. Using 13 C isotopic tracing, we...
Abstract Early childhood tumours arise from transformed embryonic cells, which often carry large copy number alterations (CNA). However, it remains unclear how CNAs contribute to tumourigenesis due a lack of suitable models. Here we employ female human stem cell (hESC) differentiation and single-cell transcriptome epigenome analysis assess the effects chromosome 17q/1q gains, are prevalent in embryonal tumour neuroblastoma (NB). We show that impair specification trunk neural crest (NC) cells...
ABSTRACT Micrococcal nuclease sequencing (MNase-seq) is the state-of-the-art method for determining chromatin structure and nucleosome positioning. Data analysis complex due to AT-dependent sequence bias of endonuclease, requirement high depth. Here, we present newly developed nucleosome-based MNase accessibility (nucMACC) pipeline unveiling regulatory landscape by measuring stability. nucMACC represents first systematic, genome-wide approach detecting unstable (“fragile”) nucleosomes. We...