Falk Hertwig
A5076037735- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Cancer therapeutics and mechanisms
- Microtubule and mitosis dynamics
- Chromatin Remodeling and Cancer
- Neuroendocrine Tumor Research Advances
- Ubiquitin and proteasome pathways
- Brain Tumor Detection and Classification
- Lung Cancer Research Studies
- Cancer Genomics and Diagnostics
- interferon and immune responses
- Lung Cancer Treatments and Mutations
- Cancer-related molecular mechanisms research
- TGF-β signaling in diseases
- Tissue Engineering and Regenerative Medicine
- Cancer-related gene regulation
- Cancer Cells and Metastasis
- Cardiac electrophysiology and arrhythmias
- Protein Degradation and Inhibitors
- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- Cell death mechanisms and regulation
Humboldt-Universität zu Berlin
2023-2025
Charité - Universitätsmedizin Berlin
2017-2025
Freie Universität Berlin
2012-2025
University Hospital Cologne
2013-2025
University of Cologne
2013-2025
Uniwersytecki Szpital Dziecięcy
2025
Children's Clinical University Hospital
2025
German Cancer Society
2017-2023
German Cancer Research Center
2018-2023
Heidelberg University
2018-2023
Gene expression profiling is being widely applied in cancer research to identify biomarkers for clinical endpoint prediction. Since RNA-seq provides a powerful tool transcriptome-based applications beyond the limitations of microarrays, we sought systematically evaluate performance RNA-seq-based and microarray-based classifiers this MAQC-III/SEQC study prediction using neuroblastoma as model.We generate gene profiles from 498 primary neuroblastomas both 44 k microarrays. Characterization...
A systematic look at a childhood tumor Neuroblastomas—the most common type in infants—develop from fetal nerve cells, and their clinical course is highly variable. Some neuroblastomas are fatal despite treatment, whereas others respond well to treatment some undergo spontaneous regression without treatment. Ackermann et al. sequenced more than 400 pretreatment identified molecular features that characterize the three distinct outcomes. Low-risk tumors lack telomere maintenance mechanisms,...
Abstract MYCN amplification drives one in six cases of neuroblastoma. The supernumerary gene copies are commonly found on highly rearranged, extrachromosomal circular DNA (ecDNA). exact amplicon structure has not been described thus far and the functional relevance its rearrangements is unknown. Here, we analyze using short-read Nanopore sequencing chromatin landscape ChIP-seq, ATAC-seq Hi-C. This reveals two distinct classes amplicons which explain regulatory requirements for...
Abstract Human brain tumor stem cells have been enriched using antibodies against the surface protein CD133. An antibody recognizing CD133 also served to isolate normal neural from fetal human brain, suggesting a possible lineage relationship between and cells. Whether CD133-positive can be derived or progenitor still requires direct experimental evidence, an important step toward such investigations is identification characterization of CD133-presenting in neurogenic regions embryonic adult...
Abstract Intratumour heterogeneity is a major cause of treatment failure in cancer. We present in-depth analyses combining transcriptomic and genomic profiling with ultra-deep targeted sequencing multiregional biopsies 10 patients neuroblastoma, devastating childhood tumour. observe high spatial temporal somatic mutations copy-number alterations which are reflected on the level. Mutations some druggable target genes including ALK FGFR1 heterogeneous at diagnosis and/or relapse, raising issue...
Abstract Background Development of resistance to targeted therapies has tempered initial optimism that precision oncology would improve poor outcomes for cancer patients. Resistance mechanisms, however, can also confer new resistance-specific vulnerabilities, termed collateral sensitivities. Here we investigated anaplastic lymphoma kinase (ALK) inhibitor in neuroblastoma, a childhood frequently affected by activating ALK alterations. Methods Genome-wide forward genetic CRISPR-Cas9 based...
The structure of the SigB-dependent general stress regulon Bacillus subtilis has previously been characterized by proteomics approaches as well DNA array-based expression studies. However, comparing SigB targets published in three previous major transcriptional profiling studies it is obvious that although each them identified above 100 target genes, only 67 were all These substantial differences can likely be attributed to different strains, growth conditions, microarray platforms and...
Telomere maintenance is a hallmark of high-risk neuroblastoma; however, the contribution telomerase and alternative lengthening telomeres (ALT) to clinical phenotypes has remained unclear. We aimed determine relevance activation versus ALT as biomarkers in pretreatment neuroblastoma assess potential value therapeutic target.The genomic status TERT MYCN was assessed 457 neuroblastomas by fluorescence situ hybridization. examined 273 tumors detection ALT-associated promyelocytic leukemia...
Abstract In contrast to ependymal cells located above the subventricular zone (SVZ) of adult lateral ventricle wall (LVW), spinal cord (SC) possess certain neural stem cell characteristics. The molecular basis this difference is unknown. study, antibodies against multiple surface markers were applied isolate pure populations SC and LVW cells, which allowed a direct comparison their in vitro behavior vivo gene expression profile. Isolated CD133 + /CD24 /CD45 − /CD34 from displayed...
Abstract To use next-generation sequencing technology such as RNA-seq for medical and health applications, choosing proper analysis methods biomarker identification remains a critical challenge most users. The US Food Drug Administration (FDA) has led the Sequencing Quality Control (SEQC) project to conduct comprehensive investigation of 278 representative data pipelines consisting 13 sequence mapping, three quantification, seven normalization methods. In this article, we focused on impact...
Abstract Circular RNAs (circRNAs) are a regulatory RNA class. While cancer-driving functions have been identified for single circRNAs, how they modulate gene expression in cancer is not well understood. We investigate circRNA the pediatric malignancy, neuroblastoma, through deep whole-transcriptome sequencing 104 primary neuroblastomas covering all risk groups. demonstrate that MYCN amplification, which defines subset of high-risk cases, causes globally suppressed biogenesis directly...
Neuroblastoma is an embryonal pediatric tumor that originates from the developing sympathetic nervous system and shows a broad range of clinical behavior, ranging fatal progression to differentiation into benign ganglioneuroma. In experimental neuroblastoma systems, retinoic acid (RA) effectively induces neuronal differentiation, RA treatment has been therefore integrated in current therapies. However, molecular mechanisms underlying are still poorly understood. We here investigated role...
The prognostic relevance of chromosome 17 gain in neuroblastoma is still discussed. This investigation specifies the frequency, type, size, and transcriptional a large patient cohort. Primary tumor material 202 patients was analyzed using high-resolution oligonucleotide array-based comparative genomic hybridization (aCGH) correlated with clinical survival data. A subset (n = 145) for differentially expressed genes (DEG) by microarray analysis. Chromosome aCGH analysis showed numerical 94/202...
<title>Abstract</title> Purpose Segmental gain of chromosome 17q is the most common genetic aberration in high-risk neuroblastoma, but its role disease progression poorly understood. This study aims to address contribution neuroblastoma malignancy. Patients and methods: We analyzed transcriptional landscape 417 patients across various risk groups clinical stages using multi-omic approaches. Single-cell RNA/DNA sequencing SNP arrays were combined characterize genomic aberrations, while...