A5011870789- Zebrafish Biomedical Research Applications
- Amyotrophic Lateral Sclerosis Research
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Neurogenetic and Muscular Disorders Research
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- Fungal and yeast genetics research
- Congenital heart defects research
- Neurogenesis and neuroplasticity mechanisms
- Retinal Development and Disorders
- RNA Research and Splicing
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Planarian Biology and Electrostimulation
- Spinal Cord Injury Research
- Physiological and biochemical adaptations
- Neurobiology and Insect Physiology Research
- Axon Guidance and Neuronal Signaling
- MicroRNA in disease regulation
- Visual perception and processing mechanisms
- Photoreceptor and optogenetics research
National Institute of Genetics
2015-2025
Tokyo Medical University
2019-2022
The Graduate University for Advanced Studies, SOKENDAI
2008-2020
Jikei University School of Medicine
2004-2010
National Institute for Physiological Sciences
2010
Cancer Research UK
2005-2007
The Honourable Society of Lincoln's Inn
2005-2007
The University of Tokyo
2001-2002
Targeted gene expression is a powerful approach to study the function of genes and cells in vivo. In Drosophila, P element-mediated Gal4-UAS method has been successfully used for this purpose. However, similar methods have not established vertebrates. Here we report development targeted methodology zebrafish based on Tol2 transposable element its application functional neural circuits. First, developed trap enhancer constructs carrying an engineered yeast Gal4 transcription activator...
The molecular genetic mechanisms of sex determination are not known for most vertebrates, including zebrafish. We identified a mutation in the zebrafish fancl gene that causes homozygous mutants to develop as fertile males due female-to-male reversal. Fancl is member Fanconi Anemia/BRCA DNA repair pathway. Experiments showed was expressed developing germ cells bipotential gonads at critical time sexual fate determination. Caspase-3 immunoassays revealed increased cell apoptosis compromised...
Abstract Background We have developed genetic methods in zebrafish by using the Tol2 transposable element; namely, transgenesis, gene trapping, enhancer trapping and Gal4FF-UAS system. Gene trap constructs contain a splice acceptor GFP or Gal4FF (a modified version of yeast Gal4 transcription activator) gene, hsp70l promoter gene. By performing screens these constructs, we generated transgenic that express specific cells, tissues organs. expression is visualized creating double fish carrying...
Targeted gene expression by the Gal4‐UAS system is a powerful methodology for analyzing function of genes and cells in vivo has been extensively used genetic studies Drosophila . On other hand, had not applied effectively to vertebrate systems long time mainly due lack an efficient transgenesis method. Recently, highly method using medaka fish Tol2 transposable element was developed zebrafish. Taking advantage transposon system, we groups Gal4 trap enhancer methods established various...
Gene trap and enhancer methods using transposon or retrovirus have been recently described in zebrafish. However, insertional mutants these not reported. We report here development of an method by the Tol2 transposable element identification characterization mutants. created 73 fish lines that carried single copy insertions construct, which contained zebrafish hsp70 promoter GFP gene, their genome expressed specific cells, tissues organs, indicating is highly capable responding to...
In fish, amino acids are food-related important olfactory cues to elicit an attractive response. However, the neural circuit underlying this behavior is not fully elucidated. present study, we applied Tol2 transposon-mediated gene trap method dissect zebrafish system genetically. Four lines (SAGFF27A, SAGFF91B, SAGFF179A, and SAGFF228C) were established in which modified transcription activator Gal4FF was expressed distinct subsets of sensory neurons (OSNs). The OSNs individual projected...
Abstract Cytoplasmic aggregation of TDP-43 characterizes degenerating neurons in most cases amyotrophic lateral sclerosis (ALS). Here, we develop an optogenetic variant (opTDP-43), whose multimerization status can be modulated vivo through external light illumination. Using the translucent zebrafish neuromuscular system, demonstrate that short-term stimulation reversibly induces cytoplasmic opTDP-43 mislocalization, but not aggregation, spinal motor neuron, leading to axon outgrowth defect...
Inherited retinal dystrophies (IRDs) are progressive diseases leading to vision loss. Mutation in the eyes shut homolog (EYS) gene is one of most frequent causes IRD. However, mechanism photoreceptor cell degeneration by mutant EYS has not been fully elucidated. Here, we generated organoids from induced pluripotent stem cells (iPSCs) derived patients with EYS-associated dystrophy (EYS-RD). In RD organoids, both and G protein–coupled receptor kinase 7 (GRK7), proteins handling phototoxicity,...
The most frequent genetic cause of frontotemporal lobar degeneration (FTLD) and amyotrophic lateral sclerosis (ALS) is the expansion a GGGGCC hexanucleotide repeat in non-coding region chromosome 9 open reading frame 72 (C9orf72) locus. pathological hallmarks observed C9orf72 carriers are formation RNA foci deposition dipeptide (DPR) proteins derived from associated non-ATG (RAN) translation. Currently, it unclear whether foci, DPR translation products, or partial loss predominantly drive...
Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, hence is laborious cumbersome, especially when dealing a large number samples. Here we describe rapid reliable method for preparing extract from yeast, one which applicable to routine western blotting.
Spatially distributed sensory information is topographically mapped in the brain by point-to-point correspondence of connections between peripheral receptors and central target neurons. In fishes, for example, axonal projections from mechanosensory lateral line organize a somatotopic neural map. The provides hydrodynamic intricate behaviors such as navigation prey detection. It also mediates fast startle reactions triggered Mauthner cell. However, it not known how lateralis map built to...
In the brain of adult mammals, neuronal precursors are generated in subventricular zone lateral wall ventricles and migrate into olfactory bulbs (OBs) through a well-studied route called rostral migratory stream (RMS). Recent studies have revealed that comparable neural stem cell niche is widely conserved at ventricular vertebrates. However, little known about migration nonmammalian brains. Here, we show that, zebrafish, cluster telencephalic migrates OB via equivalent to mammalian RMS....
The Tol2 transposable element is a powerful genetic tool in model vertebrates and has been used for transgenesis, insertional mutagenesis, gene trapping, enhancer trapping. However, an vivo transposition system using not yet developed. Here we report the vertebrate, zebrafish. First, constructed transgenic zebrafish that carried single-copy integrations of on genome injected transposase mRNA into one-cell stage embryos. insertions were mobilized efficiently germ lineage. We then insertion...
To functionally evaluate novel human sequence-derived candidate genes and variants for unsolved ocular congenital cranial dysinnervation disorders (oCCDDs). Through exome genome sequencing of a genetically oCCDD cohort, we previously reported the identification in many genes. Here, describe parallel study that prioritized subset these (43 genes, 57 zebrafish genes) using G0 CRISPR/Cas9-based knockout assay generated F2 germline mutants 17. We tested functionality uncertain significance known...
Abstract Exit from mitosis requires the inactivation of cyclin-dependent kinase (CDK) activity. In budding yeast Saccharomyces cerevisiae, a number gene products have been identified as components signal transduction network regulating CDK (called MEN, for mitotic exit network). Cdc15, one such is an essential protein kinase. By two-hybrid screening, we Cdc15 binding Tem1 GTPase, another regulator MEN. Coprecipitation experiments revealed that binds to in vivo. deletion analysis, found...