A5060835082- Congenital heart defects research
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Zebrafish Biomedical Research Applications
- Blood properties and coagulation
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Environmental Toxicology and Ecotoxicology
- Marine animal studies overview
- Cardiac Valve Diseases and Treatments
- Cardiac electrophysiology and arrhythmias
- Hearing, Cochlea, Tinnitus, Genetics
- Pulmonary Hypertension Research and Treatments
- Cardiomyopathy and Myosin Studies
- Retinal Development and Disorders
- Coronary Artery Anomalies
- Pesticide Exposure and Toxicity
- Aortic Disease and Treatment Approaches
- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Advanced Fluorescence Microscopy Techniques
- Cardiac Fibrosis and Remodeling
- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- Multilevel Inverters and Converters
Inserm
2016-2025
Université de Montpellier
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Institut de Génomique Fonctionnelle
2013-2025
Laboratoire d'Excellence Canaux Ioniques d'Intérêt Thérapeutique
2019-2024
Laboratoire Univers et Particules de Montpellier
2022
Centre for Genomic Regulation
2009-2018
Center of Regenerative Medicine in Barcelona
2012
Senso (Spain)
2012
Hubrecht Institute for Developmental Biology and Stem Cell Research
2007
Hypoxia plays an important role in many biological/pathological processes. In particular, hypoxia is associated with cardiac ischemia. which, although initially inducing a protective response, will ultimately lead to the death of cardiomyocytes and loss tissue, severely affecting functionality. Although myocardial damage/loss remains insurmountable problem for adult mammals, same not true zebrafish, which are able completely regenerate their heart after extensive injury. Myocardial...
Mechanosensitivity is an inherent property of virtually all cell types, allowing them to sense and respond physical environmental stimuli. Stretch-activated ion channels represent a class mechanosensitive proteins which allow cells rapidly changes in membrane tension; however their identity has remained elusive. The piezo genes have recently been identified as family stretch-activated channels. We set out determine the role piezo1 during zebrafish development. Here we report that...
Hair cells in the inner ear display a characteristic polarization of their apical stereocilia across plane sensory epithelium. This planar orientation allows coherent transduction mechanical stimuli because axis morphological polarity corresponds to direction excitability hair cells. Neuromasts lateral line fishes and amphibians form two intermingled populations oriented at 180 degrees relative each other, however, creating stimulus-polarity ambiguity. Therefore, it is unknown how these...
The sense of touch allows an organism to detect and respond physical environmental stimuli. Mechanosensitive proteins play a crucial role in this process by converting the mechanical cue into biological response. Recently, Piezo family stretch-activated ion channels has been identified as genuine mechanosensitive proteins. We set out determine whether any these genes are involved response during zebrafish development. In situ hybridization indicates that piezo2b is specifically expressed...
Phenylazopyridines are photoisomerizable compounds with high potential to control biological functions light. We have obtained a series of phenylazopyridines light dependent activity as negative allosteric modulators (NAM) metabotropic glutamate receptor subtype 5 (mGlu5). Here we describe the factors needed achieve an operational molecular photoisomerization and its effective translation into in vitro vivo photoswitching, which includes zebrafish larva motility regulation antinociceptive...
The oculocerebrorenal syndrome of Lowe (OCRL) is a rare X-linked disorder characterized by severe mental retardation, congenital cataracts and renal Fanconi syndrome. OCRL1 protein phosphatidylinositol 4,5-bisphosphate 5-phosphatase with C-terminal RhoGAP domain. Considering the pleiotropic cellular functions Rho GTPases (Rho, Rac Cdc42) their dysregulation in several forms we have investigated so far unexplored function domain OCRL1. Activated GTPase was found to stably associate vitro...
Spatially distributed sensory information is topographically mapped in the brain by point-to-point correspondence of connections between peripheral receptors and central target neurons. In fishes, for example, axonal projections from mechanosensory lateral line organize a somatotopic neural map. The provides hydrodynamic intricate behaviors such as navigation prey detection. It also mediates fast startle reactions triggered Mauthner cell. However, it not known how lateralis map built to...
ABSTRACT Neural damage is a devastating outcome of physical trauma. The glia are one the main effectors neuronal repair in nervous system, but dynamic interactions between peripheral neurons and Schwann cells during injury regeneration remain incompletely characterized. Here, we combine laser microsurgery, genetic analysis, high-resolution intravital imaging lattice light-sheet microscopy to study interaction sensory zebrafish model neurotrauma. We found that chronic denervation by ablation...
Aim: Blood flow-induced mechanical forces, particularly wall shear stress (WSS), play a fundamental role in aortic valve remodeling and maturation. Dysregulation of these processes contributes to age-related diseases, such as stenosis regurgitation. While epidermal growth factor receptor (EGFR) signaling has been implicated development, its mechanotransduction remains unclear. This study aims investigate how EGFR regulates WSS-induced valvular cells explore interaction with the...
Oculocerebrorenal Lowe syndrome is a rare X-linked disorder characterized by bilateral cataract, mental retardation and renal Fanconi syndrome. The protein Ocrl1 PIP2 5-phosphatase, primarily localized to the trans-Golgi network (TGN), which 'loss of function' mutations result in accumulation patient's cells. Although involved many cell functions including signalling, vesicle trafficking actin polymerization, it has been difficult so far decipher molecular/cellular mechanisms responsible for...
Directional mechanoreception by hair cells is transmitted to the brain via afferent neurons enable postural control and rheotaxis. Neuronal tuning individual directions of mechanical flow occurs when each peripheral axon selectively synapses with multiple identical planar polarization. How such mechanosensory labeled lines are established maintained remains unsolved. Here, we use zebrafish lateral line reveal that asymmetric activity transcription factor Emx2 diversifies cell identity...
Migration of GnRH neurons, a critical step for vertebrate reproduction, depends on circuit-intrinsic synaptic communication.
Abstract Bicuspid aortic valve (BAV), the most common cardiovascular malformation occurs in 0.5–1.2% of population. Although highly heritable, few causal mutations have been identified BAV patients. Here, we report targeted sequencing HOXA1 a cohort patients and identification rare indel variants homopolymeric histidine tract HOXA1. In vitro analysis shows that disruption this motif leads to significant reduction protein half-life defective transcriptional activity zebrafish, targeting...
The modular Gal4/UAS gene expression system has become an indispensable tool in modern biology. Several large-scale gene- and enhancer-trap screens the zebrafish have generated hundreds of transgenic lines expressing Gal4 unique patterns. However, early embryonic severely limits their use for studies on regeneration or behavior because UAS-driven effectors could disrupt normal organogenesis. To overcome this limitation, we explored repressor Gal80 transient assays with stable transgenes to...
Mechanosensors are emerging players responding to hemodynamic and physical inputs. Their significance in the central nervous system remains relatively uncharted. Using human-derived brain specimens or cells a pre-clinical model of mesio-temporal lobe epilepsy (MTLE), we examined how mRNA levels mechanosensitive channel PIEZO1 adjust disease-associated pro-inflammatory trajectories. In tissue micro-punches obtained from 18 drug-resistant MTLE patients, expression positively correlated with...
Fishes and amphibians localize hydromechanical variations along their bodies using the lateral-line sensory system. This is possible because spatial distribution of neuromasts represented in hindbrain by a somatotopic organization lateralis afferent neurons' central projections. The mechanisms that establish somatotopy are not known. Using BAPTI neuronal tracing zebrafish, we demonstrate growth anisotropy posterior ganglion. We characterized new transgenic line for vivo imaging to show...
Cone snails produce a fast-acting and often paralyzing venom that is usually injected into their prey or predator through hypodermic needle-like modified radula tooth. Many diverse compounds are found in including small molecules, peptides enzymes. However, peptidic toxins called conotoxins (10–40 residues 2–4 disulfide bonds) largely dominate these cocktails. These rich very valuable pharmacological tools for investigating the function of ions channels, G-protein coupled receptors,...