A5068539894- Growth Hormone and Insulin-like Growth Factors
- Hypothalamic control of reproductive hormones
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Pancreatic function and diabetes
- Neuropeptides and Animal Physiology
- Neuroendocrine regulation and behavior
- Reproductive biology and impacts on aquatic species
- Photoreceptor and optogenetics research
- Regulation of Appetite and Obesity
- Neurobiology and Insect Physiology Research
- Stress Responses and Cortisol
- Circadian rhythm and melatonin
- Pituitary Gland Disorders and Treatments
- Biochemical Analysis and Sensing Techniques
- Congenital heart defects research
- Diabetes and associated disorders
- Birth, Development, and Health
- Adipose Tissue and Metabolism
- Neurogenesis and neuroplasticity mechanisms
- Cardiac electrophysiology and arrhythmias
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neural Engineering
- Reproductive Biology and Fertility
Institut de Génomique Fonctionnelle
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Université de Montpellier
2015-2024
Inserm
2015-2024
Genomics (United Kingdom)
2011-2024
Montpellier GenomiX
2017
Institut National de Recherche en Santé Publique
2011
Centre National pour la Recherche Scientifique et Technique (CNRST)
2011
Hôpital Arnaud de Villeneuve
2005
University of Zurich
2001
To maintain homeostasis, hypothalamic neurons in the arcuate nucleus must dynamically sense and integrate a multitude of peripheral signals. Blood-borne molecules therefore be able to circumvent tightly sealed vasculature blood-brain barrier rapidly access their target neurons. However, how information encoded by circulating appetite-modifying hormones is conveyed central remains largely unexplored. Using vivo multiphoton microscopy together with fluorescently labeled ligands, we demonstrate...
All higher life forms critically depend on hormones being rhythmically released by the anterior pituitary. The proper functioning of this master gland is dynamically controlled a complex set regulatory mechanisms that ultimately determine fine tuning excitable endocrine cells, all them heterogeneously distributed throughout gland. Here, we provide evidence for an intrapituitary communication system which information transferred via network nonendocrine folliculostellate (FS) cells. Local...
A compound's intratumoural distribution is an important determinant for the effectiveness of molecular therapy or imaging. Antibodies (Abs), though often used in design targeted compounds, struggle to achieve a homogenous due their large size and bivalent binding mechanism. In contrast, smaller compounds like nanobodies (Nbs) are expected distribute more homogenously, this has yet be demonstrated vivo at microscopic level. We propose intravital approach evaluate different fluorescently...
Experience-dependent plasticity of cell and tissue function is critical for survival by allowing organisms to dynamically adjust physiological processes in response changing or harsh environmental conditions. Despite the conferred evolutionary advantage, it remains unknown whether emergent experience-dependent properties are present populations organized as networks within endocrine tissues involved regulating body-wide homeostasis. Here we show, using lactation repeatedly activate a...
Pituitary growth hormone (GH)-secreting cells regulate and metabolism in animals humans. To secrete highly ordered GH pulses (up to 1,000-fold rise levels vivo), the pituitary cell population needs mount coordinated responses secretagogues, yet display an apparently heterogeneous scattered distribution 2D histological studies. address this paradox, we analyzed 3D both positioning signaling of using reconstructive, two-photon excitation microscopy image entire GH-EGFP transgenic mice. Our...
The pituitary gland has long been considered to be a random patchwork of hormone-producing cells. By using pituitary-scale tridimensional imaging for two the least abundant cell lineages, corticotropes and gonadotropes, we have now uncovered highly organized interdigitated networks that reflect homotypic heterotypic interactions between Although newly differentiated corticotrope cells appear on ventral surface gland, they rapidly form strands extend from lateral tips anterior along its into...
Tuberoinfundibular dopamine (TIDA) neurons are the central regulators of prolactin (PRL) secretion. Their extensive functional plasticity allows a change from low PRL secretion in non-pregnant state to condition hyperprolactinemia that characterizes lactation. To allow this rise PRL, TIDA thought become unresponsive at lactation and functionally silenced. Here we show that, contrary expectations, electrical properties system were not modified during remained electrically responsive stimulus,...
Reorganization of the neurovascular unit has been suggested in epileptic brain, although dynamics and functional significance remain unclear. Here, we tracked vivo perivascular mural cells as a function electroencephalogram (EEG) activity following status epilepticus. We segmented cortical vascular bed to provide size- type-specific analysis cell plasticity topologically. find that are added removed from veins, arterioles, capillaries after seizure induction. Loss is proportional severity...
In order to define the differences in distribution of cytosolic free Ca2+ ([Ca2+]i) pancreatic beta-cells stimulated with fuel secretagogue glucose or Ca(2+)-mobilizing agents carbachol and ATP, we applied digital video imaging loaded fura-2.83% cells responded an increase [Ca2+]i after a latency 117 +/- 24 s (mean S.E., 85 cells). Of these cells, 16% showed slow wave oscillations (frequency 0.35/min). assess relationship between membrane potential rise, image analysis perforated patch-clamp...
Smooth muscle cells were isolated from the longitudinal layer of pregnant rat myometrium (18-19 days) and studied either freshly dissociated or during short-term primary culture (until 30 h) using intracellular microelectrode techniques direct microscopic observation. The myometrial excluded trypan blue vital stain could repetitively contract in response to various stimuli. Electrophysiological studies at 37 degrees C showed normal resting potential (-54.5 +/- 7.5 mV, n = 71). Action...
Spontaneous and CRF-stimulated changes in the cytosolic free calcium concentration ([Ca2+]i) were studied two types of corticotrophs: 1) cultured human ACTH-secreting pituitary adenoma cells (hACTH cells), 2) identified small ovoid corticotrophs from normal rat pituitaries. [Ca2+]i was monitored individual by dual emission microspectrofluorimetry using indo-1 as intracellular fluorescent Ca2+ probe. In hACTH cells, measurements carried out combination with electrophysiological recordings...
In mammals, males and females exhibit anatomical, hormonal, metabolic differences. A major example of such sex dimorphism in mouse involves hepatic drug metabolism, which is also a noticeable target circadian timekeeping. However, whether the clock itself contributes to sex-biased metabolism has remained unknown, although several daily output parameters differ between sexes number species, including humans. Here we show that dimorphic liver altered when regulators Cryptochromes, Cry1 Cry2,...
There are well-recognized sex differences in many pituitary endocrine axes, usually thought to be generated by gonadal steroid imprinting of the neuroendocrine hypothalamus. However, recognition that growth hormone (GH) cells arranged functionally organized networks raises possibility responses network different males and females. We studied this directly monitoring calcium an identical GH-releasing (GHRH) stimulus populations individual GH slices taken from male female murine GH-eGFP...
Ghrelin targets the arcuate nucleus, from where growth hormone releasing (GHRH) neurones trigger GH secretion. This hypothalamic nucleus also contains neuropeptide Y (NPY) neurons which play a master role in effect of ghrelin on feeding. Interestingly, connections between NPY and GHRH have been reported, leading to hypothesis that axis feeding circuits might be co-regulated by ghrelin.Here, we show stimulates firing rate identified neurons, transgenic GHRH-GFP mice. stimulation is prevented...
Growth hormone (GH) exerts its actions via coordinated pulsatile secretion from a GH cell network into the bloodstream. Practically nothing is known about how receives inputs in vivo and releases hormones pituitary capillaries to shape pulses. Here we have developed approaches measure local blood flow, oxygen partial pressure, activity at single-cell resolution mouse glands situ. When secretagogue (GHRH) distribution was modeled with fluorescent markers injected either bloodstream or nearby...
The pattern of prolactin (PRL) secretion depends on the physiological state. Due to insufficient detection sensitivity existing assays, precise description these patterns in mice is lacking. We described an ultrasensitive ELISA assay that can detect mouse PRL small fractions whole blood, allowing longitudinal studies profiles freely moving mice. Over a 24-hour period, males displayed no oscillation levels, whereas virgin and lactating females showed large pulses. Peaks reached 30–40 ng/mL...
In response to physiological demand, the pituitary gland generates new hormone-secreting cells from committed progenitor throughout life. It remains unclear what extent stem (PSCs), which uniquely express SOX2, contribute growth and renewal. Moreover, neither signals that drive proliferation nor their sources have been elucidated. We used genetic approaches in mouse, showing WNT pathway is essential for of all lineages gland. reveal SOX2 + are a key source ligands. By blocking secretion WNTs...
Abstract The sense of smell is mediated by the initiation action potential in olfactory sensory neurons during odor stimulation. However, little known about odorant‐olfactory receptor (OR) recognition mechanisms. In present work, we identified structural motifs odorant molecules required to activate mouse OR912‐93 detection response using calcium measurement cells transfected with OR and Gα q 15 proteins. use sets odorants led identification ketones an aliphatic carbon chain length ≥ four...