A5068836559- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Spaceflight effects on biology
- Light effects on plants
- Sleep and Wakefulness Research
- Growth Hormone and Insulin-like Growth Factors
- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- interferon and immune responses
- Neuroendocrine regulation and behavior
- Genetics, Aging, and Longevity in Model Organisms
- Retinal Development and Disorders
- Neurogenesis and neuroplasticity mechanisms
- Hypothalamic control of reproductive hormones
- S100 Proteins and Annexins
- Mitochondrial Function and Pathology
- Neurobiology and Insect Physiology Research
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Olfactory and Sensory Function Studies
- Cancer, Hypoxia, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Psychological and Temporal Perspectives Research
Institut de Génomique Fonctionnelle
2010-2024
Inserm
2011-2024
Université de Montpellier
2009-2024
Centre National de la Recherche Scientifique
2010-2024
Genomics (United Kingdom)
2011
Erasmus MC
2008
Erasmus University Rotterdam
2002-2008
Hôpital Arnaud de Villeneuve
2005
University of Vermont
2000
Concerted alteration of immune and metabolic homeostasis underlies several inflammation-related pathologies, ranging from syndrome to infectious diseases. Here, we explored the coordination nucleic acid-dependent inflammatory responses homeostasis. We reveal that STING (stimulator interferon genes) protein regulates through inhibition fatty acid desaturase 2 (FADS2) rate-limiting enzyme in polyunsaturated (PUFA) desaturation. ablation agonist-mediated degradation increased FADS2-associated...
The circadian clock comprises a set of genes involved in cell-autonomous transcriptional feedback loops that orchestrate the expression range downstream genes, driving patterns behavior. Cognitive dysfunction, mood disorders, anxiety and substance abuse disorders have been associated with disruptions rhythm but causal relationship these associations is still poorly understood. In present study, we investigate effect genetic disruption clock, through deletion both paralogs core gene...
Pituitary growth hormone (GH)-secreting cells regulate and metabolism in animals humans. To secrete highly ordered GH pulses (up to 1,000-fold rise levels vivo), the pituitary cell population needs mount coordinated responses secretagogues, yet display an apparently heterogeneous scattered distribution 2D histological studies. address this paradox, we analyzed 3D both positioning signaling of using reconstructive, two-photon excitation microscopy image entire GH-EGFP transgenic mice. Our...
Tuberoinfundibular dopamine (TIDA) neurons are the central regulators of prolactin (PRL) secretion. Their extensive functional plasticity allows a change from low PRL secretion in non-pregnant state to condition hyperprolactinemia that characterizes lactation. To allow this rise PRL, TIDA thought become unresponsive at lactation and functionally silenced. Here we show that, contrary expectations, electrical properties system were not modified during remained electrically responsive stimulus,...
The pattern of prolactin (PRL) secretion depends on the physiological state. Due to insufficient detection sensitivity existing assays, precise description these patterns in mice is lacking. We described an ultrasensitive ELISA assay that can detect mouse PRL small fractions whole blood, allowing longitudinal studies profiles freely moving mice. Over a 24-hour period, males displayed no oscillation levels, whereas virgin and lactating females showed large pulses. Peaks reached 30–40 ng/mL...
In mammals, males and females exhibit anatomical, hormonal, metabolic differences. A major example of such sex dimorphism in mouse involves hepatic drug metabolism, which is also a noticeable target circadian timekeeping. However, whether the clock itself contributes to sex-biased metabolism has remained unknown, although several daily output parameters differ between sexes number species, including humans. Here we show that dimorphic liver altered when regulators Cryptochromes, Cry1 Cry2,...
There are well-recognized sex differences in many pituitary endocrine axes, usually thought to be generated by gonadal steroid imprinting of the neuroendocrine hypothalamus. However, recognition that growth hormone (GH) cells arranged functionally organized networks raises possibility responses network different males and females. We studied this directly monitoring calcium an identical GH-releasing (GHRH) stimulus populations individual GH slices taken from male female murine GH-eGFP...
Attempts to understand circadian organization in the mammalian retina have concentrated increasingly on mouse. However, rather little is known regarding control of retinal light responses this species. Here, authors address deficit using electroretinogram (ERG) recordings C57BL/6 mice evaluate rhythmicity wild-type and identify consequences clock loss Cry1 — /— Cry2 —/— mice. They observe a rhythm ERG waveform under light-adapted, cone-isolating conditions mice, with b-wave speed amplitude...
We investigated the organization of spontaneous rises in cytosolic free Ca2+ concentration ([Ca2+] i) due to electrical activity acute pituitary slices. Real time confocal imaging revealed that 73% cells generated fast peaking [Ca2+] i transients. Strikingly, groups apposing enhanced their synchrony with a speed coactivation >1,000 μm/s. Single-cell injection Neurobiotin or Lucifer yellow labeled clusters cells, which corresponded coactive cells. Halothane, gap junction blocker, markedly...
In stable transfection experiments in the GH-producing GC cell line, a construct containing entire signal peptide and first 22 residues of human GH linked frame with enhanced green fluorescent protein (eGFP), produced brightly cells granular distribution eGFP. This eGFP reporter was then inserted into 40-kb cosmid transgene locus control region for hGH gene used to generate transgenic mice. Anterior pituitaries from these GH-eGFP mice showed numerous clusters strongly cells, which were also...
The mammalian circadian system is composed of multiple peripheral clocks that are synchronized by a central pacemaker in the suprachiasmatic nuclei hypothalamus. This keeps track external world rhythms through entrainment various time cues, such as light-dark cycle and feeding schedule. Alterations photoperiod meal modulate phase coupling between oscillators. In this study, we used real-time quantitative PCR to assess clock gene expression liver pituitary gland from mice raised under...
Angiogenesis contributes in multiple ways to disease progression tumors and reduces treatment efficiency. Molecular therapies targeting Vegf signaling combined with chemotherapy or other drugs exhibit promising results improve efficacy of treatment. Dopamine has been recently proposed be a novel safe anti-angiogenic drug that stabilizes abnormal blood vessels increases therapeutic efficacy. Here, we aimed identify normalize tumoral restore normal perfusion tumor tissue receptor inhibitor...
Endocrine cells isolated from the anterior pituitary fire intracellular Ca2+ ([Ca2+]i) transients due to voltage-gated entry. However, patterns of[ Ca2+]i within glandular parenchyma of are unknown. Here we describe, using real-time confocal laser microscopy, several spontaneous calcium signaling in acute slices prepared male as well cycling and lactating female rats. Forty percent demonstrated a bursting mode, consisting an active period [Ca2+]i firing at constant frequency, followed by...
Circadian clocks drive biological rhythms with a period of approximately 24 hours and keep in time the outside world through daily resetting by environmental cues. While this external entrainment has been extensively investigated suprachiasmatic nuclei (SCN), role internal systemic rhythms, including fluctuations core temperature or circulating hormones remains debated. Here, we show that lactating mice, which exhibit dampened possess normal molecular clockwork but impaired both heat shock...
The anterior pituitary is an endocrine gland that controls basic body functions. Pituitary cell functioning depends on membrane excitability, which induces cytosolic calcium rises. Here, we reported the first identification of small‐amplitude voltage fluctuations controlled spike firing in cells recorded situ. Three patterns were distinguishable by their durations (1–100 s). These could be ordered top each other, namely response to secretagogues. Thus, express situ a code lead multimodal...
During gestation, parturition, and lactation, the endocrine axis of dam must continually adapt to ensure continual healthy development offspring. The anterior pituitary gland, which serves as interface between brain periphery, undergoes adaptations that contribute regulation reproductive axis. Growth factors their receptors are potential candidates for intrapituitary paracrine participate in functional anatomical plasticity gland. We examined involvement growth factor glial cell-derived...
By regulating the metabolism of fatty acids, carbohydrates, and xenobiotic, mammalian circadian clock plays a fundamental role on liver physiology. At present, it is supposed that regulates mostly by expression enzymes at transcriptional level. However, recent evidences suggest some signaling pathways synchronized can also influence post-transcriptional In this context, we have recently shown synchronizes rhythmic activation IRE1α pathway in endoplasmic reticulum. The absence perturbs...