A5030380459- interferon and immune responses
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Viral Infections and Vectors
- RNA modifications and cancer
- Inflammasome and immune disorders
- Immune cells in cancer
- Immune Cell Function and Interaction
Université de Montpellier
2020-2025
Centre National de la Recherche Scientifique
2020-2025
Institut de Génétique Humaine
2020-2022
Institut de Génétique Moléculaire de Montpellier
2020-2022
Concerted alteration of immune and metabolic homeostasis underlies several inflammation-related pathologies, ranging from syndrome to infectious diseases. Here, we explored the coordination nucleic acid-dependent inflammatory responses homeostasis. We reveal that STING (stimulator interferon genes) protein regulates through inhibition fatty acid desaturase 2 (FADS2) rate-limiting enzyme in polyunsaturated (PUFA) desaturation. ablation agonist-mediated degradation increased FADS2-associated...
Abstract The detection of cytosolic dsDNA is tightly regulated to avoid pathological inflammatory responses. A major pathway involved in their relies on the cyclic GMP-AMP synthase (cGAS) that triggers activation Stimulator interferon genes (STING) which subsequently drives expression and type I Interferons (IFNs). Here, we show methyl-CpG-binding protein 2 (MECP2), a transcriptional regulator, controls dsDNA-associated We presence promotes MECP2 export from nucleus cytosol where it...
Abstract Cytosolic DNA promotes inflammatory responses upon detection by the cyclic GMP‐AMP (cGAMP) synthase (cGAS). It has been suggested that cGAS downregulation is an immune escape strategy harnessed tumor cells. Here, we used glioblastoma cells show undetectable levels to address if alternative pathways can promote pro‐inflammatory signaling. We DNA‐PK repair complex (i) drives cGAS‐independent IRF3‐mediated type I Interferon and (ii) its catalytic activity required for cGAS‐dependent...
The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway plays a pivotal role in several cellular processes including pathogen recognition and inflammatory responses. We describe protocol to activate the cGAS-STING murine cells using nucleic acids transfection. how prepare acid probes validate activation by western blot gene expression analysis. can be applied investigate signaling both human cell lines. For complete details on use execution this protocol, please...
Summary Inflammatory disorders are major health issues in which immune function and metabolic homeostasis concertedly altered. Yet, the molecular mechanisms coordinating innate pathways homeostatic conditions poorly understood. Here, we unveil a negative regulatory feedback loop involving Stimulator of interferon genes (Sting) Fatty acid desaturase 2 (Fads2). At steady state, Sting regulates FA metabolism by repressing activity Fads2 enzyme responsible for desaturation polyunsaturated FAs...
SUMMARY Cytosolic DNAs promote inflammatory responses upon detection by the cyclic GMP-AMP (cGAMP) synthase (cGAS). It has been thus suggested that cGAS downregulation is an immune escape strategy harnessed tumor cells. Here, we used glioblastoma cells lack to question whether alternative DNA pathways can pro-inflammatory signaling. We show DNA-PK repair complex drives independent but its catalytic activity required for cGAS-dependent cGAMP production and optimal downstream further...