A5009016961- Hepatitis B Virus Studies
- Mosquito-borne diseases and control
- Hepatitis C virus research
- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- Animal Virus Infections Studies
- Cancer-related molecular mechanisms research
- Viral Infections and Vectors
- Plant Virus Research Studies
- Epigenetics and DNA Methylation
- interferon and immune responses
- RNA regulation and disease
- Virus-based gene therapy research
- Circular RNAs in diseases
- Biotin and Related Studies
- Hepatitis Viruses Studies and Epidemiology
- Immune Cell Function and Interaction
- vaccines and immunoinformatics approaches
- RNA Interference and Gene Delivery
- Invertebrate Immune Response Mechanisms
Institut de Génétique Moléculaire de Montpellier
2022-2025
Université de Montpellier
2021-2025
Centre National de la Recherche Scientifique
2014-2025
MRC Laboratory of Molecular Biology
2023
Stanford University
2016-2021
Institute for Translational Medicine and Liver Disease
2020
Inserm
2018-2020
Université de Strasbourg
2013-2020
Institut de Virologie
2019-2020
Stratford University
2017
MicroRNAs regulate diverse cellular processes and play an integral role in cancer pathogenesis. Genomic variation within miRNA target sites may therefore be important sources for genetic differences risk. To investigate this possibility, we mapped HapMap single nucleotide polymorphisms (SNP) to putative recognition genes dysregulated estrogen receptor-stratified breast tumors used local linkage disequilibrium patterns identify high-ranking SNPs the Cancer Genetic Markers of Susceptibility...
Many viruses interface with the autophagy pathway, a highly conserved process for recycling cellular components. For three viral infections in which constituents are proviral (poliovirus, dengue, and Zika), we developed panel of knockouts (KOs) autophagy-related genes to test components canonical pathway utilized. We discovered that each virus uses distinct set initiation components; however, all utilize gene 9 (ATG9), lipid scavenging protein, LC3 (light-chain 3), is involved membrane...
The mosquito-borne flaviviruses include important human pathogens such as dengue, Zika, West Nile, and yellow fever viruses, which pose a serious threat for global health. Recent genetic screens identified endoplasmic reticulum (ER)-membrane multiprotein complexes, including the oligosaccharyltransferase (OST) complex, critical flavivirus host factors. Here, we show that chemical modulator of OST complex termed NGI-1 has promising antiviral activity against infections. We demonstrate blocks...
Abstract Viral neuroinfections represent a major health burden for which the development of antivirals is needed. Antiviral compounds that target consequences brain infection (symptomatic treatment) rather than cause (direct-acting antivirals) constitute promising mitigation strategy requires to be investigated in relevant models. However, physiological surrogates mimicking an adult human cortex are lacking, limiting our understanding mechanisms associated with viro-induced neurological...
RNA interference (RNAi) mediated by the small interfering (siRNA) pathway is a major antiviral mechanism in insects. This triggered when double-stranded (dsRNA) produced during virus replication recognized Dicer-2, leading to formation of virus-derived siRNA duplexes. These siRNAs are loaded onto programmable nuclease Argonaute-2 (AGO2), with one strand serving as guide target and cleave fully complementary sequences viral RNAs. While generated from dsRNA, specific species targeted for...
Abstract The detection of cytosolic dsDNA is tightly regulated to avoid pathological inflammatory responses. A major pathway involved in their relies on the cyclic GMP-AMP synthase (cGAS) that triggers activation Stimulator interferon genes (STING) which subsequently drives expression and type I Interferons (IFNs). Here, we show methyl-CpG-binding protein 2 (MECP2), a transcriptional regulator, controls dsDNA-associated We presence promotes MECP2 export from nucleus cytosol where it...
The recent discovery of Hepatitis D (HDV) -like viruses across a wide range taxa led to the establishment Kolmioviridae family. Recent studies suggest that kolmiovirids can be satellites other than B virus (HBV), challenging strict HBV/HDV-association dogma. Studying whether are able replicate in any animal cell they enter is essential assess their zoonotic potential. Here, we compared replication three kolmiovirids: HDV, rodent (RDeV) and snake (SDeV) deltavirus vitro vivo . We show SDeV...
Abstract Chronic HBV infection is a major cause of liver disease and cancer worldwide. Approaches for cure are lacking, the knowledge virus-host interactions still limited. Here, we perform genome-wide gain-of-function screen using poorly permissive hepatoma cell line to uncover host factors enhancing infection. Validation studies in primary human hepatocytes identified CDKN2C as an important factor replication. overexpressed highly cells HBV-infected patients. Mechanistic show role inducing...
Hepatitis C virus (HCV) depends on liver-specific microRNA miR-122 for efficient viral RNA amplification in liver cells. This interacts with two different conserved sites at the very 5' end of RNA, enhancing stability and promoting replication RNA. Treatment HCV patients oligonucleotides that sequester resulted profound loss phase II clinical trials. However, some accumulated their sera a genome contained single cytidine to uridine mutation third nucleotide from genomic end. It is shown here...
Human translation initiation relies on the combined activities of numerous ribosome-associated eukaryotic factors (eIFs). The largest factor, eIF3, is an ∼800 kDa multiprotein complex that orchestrates a network interactions with small 40S ribosomal subunit, other eIFs, and mRNA, while participating in nearly every step initiation. How these take place during time course remains unclear. Here, we describe method for expression affinity purification fluorescently-tagged eIF3 from human cells....
ABSTRACT Glycans modify lipids and proteins to mediate inter- intramolecular interactions across all domains of life. RNA, another multifaceted biopolymer, is not thought be a major target glycosylation. Here, we challenge this view with evidence that mammalian cells use RNA as third scaffold for glycosylation in the secretory pathway. Using battery chemical biochemical approaches, find select group small noncoding RNAs including Y are modified complex, sialylated N-glycans (glycoRNAs)....
Recombinant adeno-associated virus (rAAV) vectors have the unique property of being able to perform genomic targeted integration (TI) without inducing a double-strand break (DSB). In order improve our understanding mechanism behind TI mediated by AAV and its efficiency, we performed an unbiased genetic screen in human cells using promoterless AAV-homologous recombination (AAV-HR) vector system. We identified that inhibition Fanconi anemia complementation group M (FANCM) protein enhanced...
Abstract Receptor for Activated protein C kinase 1 (RACK1) is a scaffold that has been found in association with several signaling complexes, and the 40S subunit of ribosome. Using model organism Drosophila melanogaster, we recently showed RACK1 required at ribosome internal entry site (IRES)-mediated translation viruses. Here, report proteomic characterization interactome S2 cells. We carried out Label-Free quantitation using both Data-Dependent Data-Independent Acquisition (DDA DIA,...
Summary Cytosolic dsDNAs are potent immune-stimulatory molecules that trigger inflammation in several human pathologies 1,2 . A major pathway for the detection of cytosolic dsDNA relies on cyclic GMP-AMP (cGAMP) synthase (cGAS) produces 2’3’-cGAMP dinucleotide (CDN) activation Stimulator Interferon Genes (STING) adaptor protein subsequently drives type I (IFN) responses 3,4 Here, we investigated mechanism regulating intracellular levels. We show DNA-dependent kinase catalytic subunit...