A5001069964- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Angiogenesis and VEGF in Cancer
- Antimicrobial Resistance in Staphylococcus
- RNA Research and Splicing
- Ferroptosis and cancer prognosis
- Lymphatic System and Diseases
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Biomarkers in Disease Mechanisms
- Galectins and Cancer Biology
- RNA regulation and disease
- Heat shock proteins research
- Viral Infections and Immunology Research
- Hepatitis C virus research
- Cancer, Stress, Anesthesia, and Immune Response
- Bacterial biofilms and quorum sensing
- Adipose Tissue and Metabolism
- Biochemical and Structural Characterization
- Endoplasmic Reticulum Stress and Disease
- Infective Endocarditis Diagnosis and Management
Architecture et Réactivité de l'arN
2015-2024
Université de Strasbourg
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Institut de Biologie Moléculaire et Cellulaire
2008-2023
Tufts Medical Center
2013
University of California, San Francisco
2013
Institut de génétique et de biologie moléculaire et cellulaire
2007-2009
Consejo Superior de Investigaciones Científicas
2009
Inserm
2005-2009
Unidades Centrales Científico-Técnicas
2009
Regulation of translation initiation is well appropriate to adapt cell growth in response stress and environmental changes. Many bacterial mRNAs adopt structures their 5′ untranslated regions that modulate the accessibility 30S ribosomal subunit. Structured interact with a two-step process where docking folded mRNA precedes an accommodation step. Here, we used combination experimental approaches vitro (kinetic unfolding binding experiments analyze mRNA–protein or mRNA–ribosome complexes,...
Various tumors metastasize via lymph vessels and nodes to distant organs. Even though are hypoxic, the mechanisms of how hypoxia regulates lymphangiogenesis remain poorly characterized. Here, we show that reduced vascular endothelial growth factor C (VEGF-C) transcription cap-dependent translation upregulation hypophosphorylated 4E-binding protein 1 (4E-BP1). However, initiation VEGF-C was induced by through an internal ribosome entry site (IRES)-dependent mechanism. IRES-dependent...
Abstract The human opportunistic pathogen Staphylococcus aureus produces numerous small regulatory RNAs (sRNAs) for which functions are still poorly understood. Here, we focused on an atypical and large sRNA called RsaC. Its length varies between different isolates due to the presence of repeated sequences at 5′ end while its 3′ part is structurally independent highly conserved. Using MS2-affinity purification coupled with RNA sequencing (MAPS) quantitative differential proteomics, sodA mRNA...
Crc is a key global translational regulator in Pseudomonads that orchestrates the hierarchy of induction several catabolic pathways for amino acids, sugars, hydrocarbons or aromatic compounds. In presence which are preferred carbon sources, inhibits translation Pseudomonas putida alkS and benR mRNAs, code transcriptional regulators genes required to assimilate alkanes (hydrocarbons) benzoate (an compound), respectively. binds 5′-end these but sequence and/or structure recognized, way it...
Significance This work reports unique insights into IF2 function during eubacterial translation initiation by addressing the of N domain within structure full-length factor in isolated form or ribosome bound, using crystallography, SAXS, cryo-EM, fast kinetics, and single molecule fluorescence.
The virulon of Staphyloccocus aureus is controlled by intricate connections between transcriptional and post-transcriptional regulators including proteins small non-coding RNAs (sRNAs). Many the sRNAs regulate gene expression through base-pairings with mRNAs. However, characterization direct sRNA targets in Gram-positive bacteria remained a difficult challenge. Here, we have applied adapted MS2-affinity purification approach coupled to RNA sequencing (MAPS) determine targetome RsaA S....
Bacterial translation initiation factor IF2 is a GTP-binding protein that catalyzes binding of initiator fMet-tRNA in the ribosomal P site. The topographical localization on subunits, prerequisite for understanding mechanism complex formation, has remained elusive. Here, we present model positioning 70S as determined by cleavage rRNA chemical nucleases Cu(II):1,10-orthophenanthroline and Fe(II):EDTA tethered to cysteine residues introduced into IF2. Two specific amino acids GII domain are...
The vascular endothelial growth factor VEGF-D promotes metastasis by inducing lymphangiogenesis and dilatation of the lymphatic vasculature, facilitating tumor cell extravasion. Here we report a novel level control for expression at protein translation. In human cells, colocalized with eIF4GI 4E-BP1, which can program increased initiation IRES motifs on mRNA translational complex. murine tumors, steady-state was despite overexpression dephosphorylation downregulates synthesis, suggesting...