A5033807221- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- PARP inhibition in cancer therapy
- MicroRNA in disease regulation
- Cellular transport and secretion
- Genetic and Kidney Cyst Diseases
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- Cholesterol and Lipid Metabolism
- interferon and immune responses
- Calcium signaling and nucleotide metabolism
- Phagocytosis and Immune Regulation
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Hormonal Regulation and Hypertension
- Glioma Diagnosis and Treatment
- Immune cells in cancer
- Inflammasome and immune disorders
- Lipid metabolism and disorders
- Retinoids in leukemia and cellular processes
- Apelin-related biomedical research
- Lipid metabolism and biosynthesis
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2020-2024
Centre National de la Recherche Scientifique
2019-2024
Nantes Université
2019-2024
Université d'Angers
2019-2024
Inserm
2019-2024
La Ligue Contre le Cancer
2022-2024
Oncogenesis Stress Signaling
2024
Glioblastoma stem-like cells (GSCs) compose a tumor-initiating and -propagating population remarkably vulnerable to variation in the stability integrity of lysosomal compartment. Previous work has shown that expression activity paracaspase MALT1 control GSC viability via lysosome abundance. However, underlying mechanisms remain elusive. By combining RNA sequencing (RNA-seq) with proteome-wide label-free quantification, we now report repression patient-derived GSCs alters homeostasis...
Abstract Cytosolic DNA promotes inflammatory responses upon detection by the cyclic GMP‐AMP (cGAMP) synthase (cGAS). It has been suggested that cGAS downregulation is an immune escape strategy harnessed tumor cells. Here, we used glioblastoma cells show undetectable levels to address if alternative pathways can promote pro‐inflammatory signaling. We DNA‐PK repair complex (i) drives cGAS‐independent IRF3‐mediated type I Interferon and (ii) its catalytic activity required for cGAS‐dependent...
Centriolar satellites are high-order assemblies, scaffolded by the protein PCM1, that gravitate as particles around centrosome and play pivotal roles in fundamental cellular processes notably ciliogenesis autophagy. Despite stringent control mechanisms involving phosphorylation ubiquitination, landscape of post-translational modifications shaping these structures remains elusive. Here, we report necrosulfonamide (NSA), a small molecule known for binding inactivating effector cell death...
Extracellular vesicles (EVs) are lipid-based nanosized particles that convey biological material from donor to recipient cells. EVs play key roles in glioblastoma progression because stem-like cells (GSCs) release pro-oncogenic, pro-angiogenic, and pro-inflammatory EVs. However, the molecular basis of EV remains poorly understood. Here, we report identification pseudokinase MLKL, a crucial effector cell death by necroptosis, as regulator constitutive secretion GSCs. We find genetic, protein,...
Glioblastoma is one of the most lethal forms adult cancer, with a median survival ∼15 mo. Targeting glioblastoma stem-like cells (GSCs) at origin tumor formation and relapse may prove beneficial. In situ, GSCs are nested within vascular bed in tight interaction brain endothelial cells, which positively control their expansion. Because notably addicted to apelin (APLN), sourced from surrounding stroma, APLN/APLNR nexus has emerged as druggable network. However, how this signaling axis...
Lysosomes are critical for the sustenance of glioblastoma stem-like cells (GSCs) properties. We present a protocol to enrich and purify lysosomes from patient-derived GSCs in culture. describe steps required stably express tagged lysosomal protein GSCs, mechanically lyse cells, magnetically immunopurify lysosomes, qualitatively assess these organelles. then detail procedure retrieving intact purified GSCs. also specify cell culture conditions, storage procedures, sample preparation...
Abstract Glioblastoma is one of the most lethal forms adult cancer with a median survival around 15 months. A potential treatment strategy involves targeting glioblastoma stem-like cells (GSC), which constitute cell autonomous reservoir aberrant able to initiate, maintain, and repopulate tumor mass. Here, we report that expression paracaspase mucosa-associated lymphoid tissue l (MALT1), protease previously linked antigen receptor-mediated NF-κB activation B-cell lymphoma survival, inversely...
à la diffusion de documents scientifiques niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
SUMMARY Glioblastoma stem-like cells (GSCs) compose a tumor-initiating and -propagating population, remarkably vulnerable to any variation in the stability integrity of endolysosomal compartment. Previous work showed that expression activity paracaspase MALT1 control GSC viability via lysosomal abundance. However, underlying mechanisms remain elusive. By combining RNAseq with proteome-wide label-free quantification, we now report repression patient-derived GSCs alters cholesterol...
Glioblastoma stem-like cells (GSCs) compose a tumor-initiating and -propagating population, remarkably vulnerable to any variation in the stability integrity of endolysosomal compartment. Previous work showed that expression activity paracaspase MALT1 control GSC viability via lysosomal abundance. However, underlying mechanisms remain elusive. By combining RNAseq with proteome-wide label-free quantification, we now report repression patient-derived GSCs alters cholesterol homeostasis, which...
SUMMARY Centriolar satellites are high-order assemblies, scaffolded by the protein PCM1, that gravitate as particles around centrosome and play pivotal roles in fundamental cellular processes notably ciliogenesis autophagy. Despite stringent control mechanisms involving phosphorylation ubiquitination, landscape of post-translational modifications shaping these structures remains elusive. Here, we report necrosulfonamide (NSA), a small molecule known for binding inactivating effector cell...
Abstract Extracellular vesicles (EVs) are lipid-based nano-sized particles that convey biological material from donor to recipient cells. They play key roles in tumour progression, notably glioblastoma which the subpopulation of Glioblastoma Stem-like Cells (GSCs) might represent a meaningful source tumour-derived EVs. However, mechanisms involved production and release EVs by GSCs still poorly understood. Here, we report identification MLKL, crucial effector cell death necroptosis, as...