A5023262279- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Hematopoietic Stem Cell Transplantation
- Erythrocyte Function and Pathophysiology
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Cellular Automata and Applications
- Glioma Diagnosis and Treatment
- Statistical Methods and Bayesian Inference
- Immune cells in cancer
- Hippo pathway signaling and YAP/TAZ
- Liver physiology and pathology
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- Markov Chains and Monte Carlo Methods
- Immune Cell Function and Interaction
- Bayesian Methods and Mixture Models
- Multiple Myeloma Research and Treatments
- RNA Research and Splicing
- Connective tissue disorders research
- Tissue Engineering and Regenerative Medicine
- Colorectal Cancer Treatments and Studies
- Autoimmune and Inflammatory Disorders Research
- Extracellular vesicles in disease
- Cellular transport and secretion
Université Paris Sciences et Lettres
2017-2024
Institut Curie
2017-2024
Inserm
2022-2024
ParisTech
2022-2024
Génomique Bioinformatique et Applications
2024
Cancer et génome: Bioinformatique, biostatistiques et épidémiologie des systèmes complexes
2024
École Nationale Supérieure des Mines de Paris
2024
Physique des Cellules et Cancers
2020-2024
Sorbonne Université
2018-2023
Centre National de la Recherche Scientifique
2018-2023
A large number of statistical models are "doubly-intractable": the likelihood normalising term, which is a function model parameters, intractable, as well marginal (model evidence). This means that standard inference techniques to sample from posterior, such Markov chain Monte Carlo (MCMC), cannot be used. Examples include, but not confined to, massive Gaussian random fields, autologistic and Exponential graph models. approximate schemes based on MCMC techniques, Approximate Bayesian...
Abstract Post-translational modifications are necessary for collagen precursor molecules (procollagens) to acquire final shape and function. However, the mechanism contribution of that occur outside endoplasmic reticulum Golgi not understood. We discovered VIPAR, with its partner proteins, regulate sorting lysyl hydroxylase 3 (LH3, also known as PLOD3) into newly identified post-Golgi IV carriers VIPAR-dependent is essential modification lysines in multiple types. Identification structural...
Abstract Glioblastoma (GBM) is a highly lethal type of cancer. GBM recurrence following chemoradiation typically attributed to the regrowth invasive and resistant cells. Therefore, there pressing need gain deeper understanding mechanisms underlying resistance its ability infiltrate. Using combination transcriptomic, proteomic, phosphoproteomic analyses, longitudinal imaging, organotypic cultures, functional assays, animal studies, clinical data we demonstrate that brain vasculature induce...
In the normal liver, hepatocytes form a uniquely polarised cell layer that enables movement of solutes from sinusoidal blood to canalicular bile. Whilst several cholestatic liver diseases with defects hepatocyte polarity have been identified, molecular mechanisms pathogenesis are not well defined. One example is arthrogryposis, renal dysfunction and cholestasis syndrome, which in most patients caused by VPS33B mutations. protein involved membrane trafficking interacts RAB11A at recycling...
Abstract Identifying true DNA cellular barcodes among polymerase chain reaction and sequencing errors is challenging. Current tools are restricted in the diversity of barcode types supported or analysis strategies implemented. As such, there a need for more versatile efficient extraction, as well to investigate which factors impact detection filtering best apply. Here we introduce package CellBarcode its simulation kit, CellBarcodeSim, that allows extraction range from bulk single-cell data...
Abstract Chronic myelomonocytic leukemia (CMML) is a severe myeloid malignancy with limited therapeutic options. Single-cell analysis of clonal architecture demonstrated early dominance few residual wildtype hematopoietic stem cells. Circulating cells the leukemic clone and cytokines they produce generate deleterious inflammatory climate. Our hypothesis that control component in CMML could contribute to stepping down disease progression. The present study explores contribution immature...
The cytokine erythropoietin (EPO) is a potent inducer of erythrocyte development and one the most prescribed biopharmaceuticals. action EPO on erythroid progenitor cells well established, but its direct hematopoietic stem (HSPCs) still debated. Here, using cellular barcoding, we traced differentiation hundreds single murine HSPCs, after ex vivo exposure transplantation, in five different cell lineages, observed transient occurrence high-output myeloid-erythroid-megakaryocyte-biased...
Liver transplantation is the definitive treatment of liver failure but donor organ shortage limits its availability. Stem cells are highly expandable and have potential to differentiate into any specialist cell. Use patient-derived induced Pluripotent Cells (hiPSCs) has additional advantage for regeneration therapies by removing need immunosuppression. We compared hepatocyte differentiation human embryonic stem (hESCs) hiPSCs in a mouse decellularised scaffold (3D) with standard vitro...
Abstract Background Medulloblastoma is the most common malignant pediatric brain tumor, and leptomeningeal dissemination (LMD) of medulloblastoma both portends a poorer prognosis at diagnosis incurable recurrence. The biological mechanisms underlying LMD are unclear. Abelson (ABL) tyrosine kinase family members, ABL1 ABL2, have been implicated in cancer cell migration, invasion, adhesion, metastasis, chemotherapy resistance, upstream mediators oncogene c-MYC fibroblasts lung cells. However,...
Extracellular vesicles (EVs) are lipid-based nanosized particles that convey biological material from donor to recipient cells. EVs play key roles in glioblastoma progression because stem-like cells (GSCs) release pro-oncogenic, pro-angiogenic, and pro-inflammatory EVs. However, the molecular basis of EV remains poorly understood. Here, we report identification pseudokinase MLKL, a crucial effector cell death by necroptosis, as regulator constitutive secretion GSCs. We find genetic, protein,...
Chronic myelomonocytic leukemia (CMML) is a severe myeloid malignancy with limited therapeutic options. Single-cell analysis of clonal architecture demonstrates early dominance few residual WT hematopoietic stem cells. Circulating cells the leukemic clone and cytokines they produce generate deleterious inflammatory climate. Our hypothesis that control component in CMML could contribute to stepping down disease progression. The present study explored contribution immature granulocytes...
SUMMARY Following infection, hematopoietic stem and progenitor cells (HSPCs) support immunity by increasing the rate of innate immune cell production but metabolic cues that guide this process are unknown. To address question, we developed MetaFate, a method to trace expression state developmental fate single in vivo . Using MetaFate identified gene program enzymes transporters confers differences myeloid differentiation potential subset HSPCs express CD62L. single-cell profiling, confirmed...
Summary Triple-negative breast cancer is associated with the worst prognosis and highest risk of recurrence among all subtypes 1 . Residual disease, formed by cells persistent to chemotherapy, remains one major clinical challenges towards full cure 2,3 There now consensus that non-genetic processes contribute chemoresistance in various tumor types, notably through initial emergence a reversible chemotolerant state 4–6 Understanding evolution stands as prerequisite for design relevant...
Abstract The cytokine erythropoietin (EPO) is a potent inducer of erythrocyte development and one the most prescribed biopharmaceuticals. action EPO on erythroid progenitor cells well established, but its direct hematopoietic stem (HSPCs) still debated. Here, using cellular barcoding, we traced differentiation hundreds single murine HSPCs, after ex vivo EPO-exposure transplantation, in five different cell lineages, observed transient occurrence high-output Myeloid-Erythroid-megaKaryocyte...