A5064800456- Cellular transport and secretion
- Platelet Disorders and Treatments
- Spatial Neglect and Hemispheric Dysfunction
- Transcranial Magnetic Stimulation Studies
- Hereditary Neurological Disorders
- Pain Management and Treatment
- Botulinum Toxin and Related Neurological Disorders
- Blood Coagulation and Thrombosis Mechanisms
- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Advanced Proteomics Techniques and Applications
- Lipid Membrane Structure and Behavior
- Microbial Inactivation Methods
- Plant Stress Responses and Tolerance
- Hemispheric Asymmetry in Neuroscience
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Venous Thromboembolism Diagnosis and Management
- Congenital heart defects research
- Pain Mechanisms and Treatments
- Supramolecular Self-Assembly in Materials
- Ergonomics and Musculoskeletal Disorders
- Sunflower and Safflower Cultivation
Stazione Zoologica Anton Dohrn
2020-2024
Azienda Ospedaliera Carlo Poma
2013-2023
Università Cattolica del Sacro Cuore
2022-2023
MRC Laboratory for Molecular Cell Biology
2010-2020
University College London
2008-2020
Nestlé (Switzerland)
2018-2020
École Polytechnique Fédérale de Lausanne
2020
Azienda Socio Sanitaria Territoriale Lariana
2019
Medical Research Council
2010-2018
University of Genoa
2017
Limb amputation may lead to chronic painful sensations referred the absent limb, ie phantom limb pain (PLP), which is likely subtended by maladaptive plasticity. The present study investigated whether transcranial direct current stimulation (tDCS), a noninvasive technique of brain that can modulate neuroplasticity, reduce PLP. In 2 double-blind, sham-controlled experiments in subjects with unilateral lower or upper amputation, we measured effects single session tDCS (2 mA, 15 min) primary...
Weibel-Palade bodies (WPBs), endothelial-specific secretory granules that are central to primary hemostasis and inflammation, occur in dimensions ranging between 0.5 5 μm. How their size is determined whether it has a functional relevance at present unknown. Here, we provide evidence for dual role of the Golgi apparatus controlling these carriers. At ministack level, cisternae constrain nanostructures ("quanta") von Willebrand factor (vWF), main WPB cargo. The ribbon architecture then allows...
Abstract Post-translational modifications are necessary for collagen precursor molecules (procollagens) to acquire final shape and function. However, the mechanism contribution of that occur outside endoplasmic reticulum Golgi not understood. We discovered VIPAR, with its partner proteins, regulate sorting lysyl hydroxylase 3 (LH3, also known as PLOD3) into newly identified post-Golgi IV carriers VIPAR-dependent is essential modification lysines in multiple types. Identification structural...
Kalirin, a multifunctional Rho GDP/GTP exchange factor, plays vital role in cytoskeletal organization, affecting process initiation and outgrowth neurons. Through alternative splicing, the Kalirin gene generates multiple functionally distinct proteins. Kalirin-7 (Kal7) is most prevalent isoform adult rat hippocampus; it terminates with postsynaptic density-95 (PSD-95)/Discs large/zona occludens-1 (PDZ) binding motif, localized to density, interacts PSD-95, causes formation of dendritic...
Changes in the size of cellular organelles are often linked to modifications their function. Endothelial cells store von Willebrand Factor (vWF), a glycoprotein essential haemostasis Weibel-Palade bodies (WPBs), cigar-shaped secretory granules that generated wide range sizes. We recently showed forcing changes WPBs modifies activity this cargo. now find endothelial treated with statins produce shorter and vWF they release at exocytosis displays reduced capability recruit platelets cell...
Highlights•The Arf-GEF GBF1 modulates anterograde trafficking of VWF and ECM proteins•Loss slows ER TGN exit, producing swollen giant WPBs•Activation via AMPK reduces endothelial WPB size secretion•Metabolic change alters cargo secretion AMPK-GBF1SummaryHow can membrane be modulated by physiological cues? A screen Golgi-associated proteins revealed that the ARF-GEF selectively modulate ER-Golgi prohaemostatic von Willebrand factor (VWF) extracellular matrix (ECM) in human cells mouse...
Abstract Background The present study investigates whether epigenetic differences emerge in the heart of patients undergoing cardiac surgery for an aortic valvular replacement (AVR) or coronary artery bypass graft (CABG). An algorithm is also established to determine how pathophysiological condition might influence human biological age. Results Blood samples and auricles were collected from who underwent procedures: 94 AVR 289 CABG. CpGs three independent blood-derived clocks selected design...
The "ribbon," a structural arrangement in which Golgi stacks connect to each other, is considered be restricted vertebrate cells. Although ribbon disruption linked various human pathologies, its functional role cellular processes remains unclear. In this study, we investigate the evolutionary origin of ribbon. We observe ribbon-like architecture cells several metazoan taxa suggesting early emergence animal evolution predating appearance vertebrates. Supported by AlphaFold2 modeling, propose...
Hormone secretion by pituitary cells is decreased roscovitine, an inhibitor of cyclin-dependent kinase 5 (Cdk5). Roscovitine treatment reorganizes cortical actin and ultrastructural analysis demonstrates that roscovitine limits the ability secretory granules to approach plasma membrane or one another. Trio, a multifunctional RhoGEF expressed in cells, interacts with peptidylglycine alpha-amidating monooxygenase, granule protein known affect cytoskeleton. inhibits Trio activate Rac, peptides...
The molecular mechanisms involved in the maturation of secretory granules, organelles that store hormones and neuropeptides, are poorly understood. As granule content proteins processed, composition membranes changes, yielding constitutive-like secretion immature producing secretagogue-responsive mature granules. Constitutive-like was not previously recognized as a process subject to regulation. We show Kalirin Trio, homologous Rho guanine nucleotide exchange factors (GEFs), which interact...
Abstract The visual system is extremely efficient at detecting events across time even very fast presentation rates; however, discriminating the identity of those much slower and requires attention over time, a mechanism with coarser resolution [Cavanagh, P., Battelli, L., & Holcombe, A. O. Dynamic attention. In C. Nobre S. Kastner (Eds.), Oxford handbook (pp. 652–675). Oxford: University Press, 2013]. Patients affected by right parietal lesion, including TPJ, are severely impaired in...
Secretory granules carrying fluorescent cargo proteins are widely used to study granule biogenesis, maturation, and regulated exocytosis. We fused the soluble secretory protein peptidylglycine alpha-hydroxylating monooxygenase (PHM) green (GFP) formation. When expressed in AtT-20 or GH3 cells, PHM-GFP fusion partitioned from endogenous hormone (adrenocorticotropic hormone, growth hormone) into separate pools. Both exogenous were stored released response secretagogue. Importantly, we found...
The field of organellar proteomics has emerged as an attempt to minimize the complexity data obtained from whole cell and tissue extracts while maximizing resolution on protein composition a single subcellular compartment. Standard methods involve lengthy density-based gradient and/or immunoaffinity purification steps followed by extraction, 1-DE or 2-DE, gel staining, in-gel tryptic digestion, identification MS. In this paper, we present alternate approach purify organelles containing...
Abstract Summary Nuclear magnetic resonance (NMR)-based metabolomics is widely used to obtain metabolic fingerprints of biological systems. While targeted workflows require previous knowledge metabolites, prior statistical analysis, untargeted approaches remain a challenge. Computational tools dealing with fully NMR-based are still scarce or not user-friendly. Therefore, we developed AlpsNMR (Automated spectraL Processing System for NMR), an R package that provides automated and efficient...
A new and short fragment-based approach towards artificial (but “natural-based”) complex polyphenols has been developed, exploiting the Ugi multicomponent reaction of phenol-containing simple substrates.
It is long established that von Willebrand factor (VWF) central to hemostasis and thrombosis. Endothelial VWF stored in cell-specific secretory granules, Weibel-Palade bodies (WPBs), organelles generated a wide range of lengths (0.5-5.0 µm). WPB size responds physiological cues pharmacological treatment, secretion from shortened WPBs dramatically reduces platelet plasma adhesion an endothelial surface.