A5071545957- Skin and Cellular Biology Research
- Nuclear Structure and Function
- Cellular transport and secretion
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Neurobiology and Insect Physiology Research
- Connective tissue disorders research
- Protein Degradation and Inhibitors
- Erythrocyte Function and Pathophysiology
- Mast cells and histamine
- Toxin Mechanisms and Immunotoxins
- Herpesvirus Infections and Treatments
- Ubiquitin and proteasome pathways
- Cystic Fibrosis Research Advances
- RNA regulation and disease
- Genomics and Chromatin Dynamics
- Transgenic Plants and Applications
- thermodynamics and calorimetric analyses
- RNA Interference and Gene Delivery
- HER2/EGFR in Cancer Research
- Insect Resistance and Genetics
- Calcium signaling and nucleotide metabolism
- Mass Spectrometry Techniques and Applications
- Developmental Biology and Gene Regulation
Securboration (United States)
2024
Queen Mary University of London
2017-2022
The Francis Crick Institute
2020-2021
University College London
2016-2018
MRC Laboratory for Molecular Cell Biology
2016-2018
Abstract Post-translational modifications are necessary for collagen precursor molecules (procollagens) to acquire final shape and function. However, the mechanism contribution of that occur outside endoplasmic reticulum Golgi not understood. We discovered VIPAR, with its partner proteins, regulate sorting lysyl hydroxylase 3 (LH3, also known as PLOD3) into newly identified post-Golgi IV carriers VIPAR-dependent is essential modification lysines in multiple types. Identification structural...
Mutations in VPS33B and VIPAS39 cause the severe multisystem disorder Arthrogryposis, Renal dysfunction Cholestasis (ARC) syndrome. Amongst other symptoms, patients with ARC syndrome suffer from ichthyosis. Roles for VIPAR have been reported lysosome-related organelle biogenesis, integrin recycling, collagen homeostasis maintenance of cell polarity. Mouse knockouts Vps33b or Vipas39 are good models develop an ichthyotic phenotype. We demonstrate that skin manifestations Vipar deficient mice...
Varicella zoster virus (VZV) is a skin-tropic that infects epidermal keratinocytes and causes chickenpox. Although common, VZV infection can be life-threatening, particularly in the immunocompromized. Therefore, understanding VZV-keratinocyte interactions important to find new treatments beyond vaccination antiviral drugs. In VZV-infected skin, kallikrein 6 ubiquitin ligase MDM2 are upregulated concomitant with keratin 10 (KRT10) downregulation. binds KRT10, targeting it for degradation via...
Abstract Keratinocyte cornification and epidermal barrier formation are tightly controlled processes, which require complete degradation of intracellular organelles, including removal keratinocyte nuclei. nuclear destruction requires Akt1-dependent phosphorylation the lamina protein, Lamin A/C, essential for integrity. However, molecular mechanisms that result in their regulation not well defined. Post-confluent cultures rat keratinocytes (REKs) undergo spontaneous differentiation, allowing...
In Drosophila epithelial cells, apical exclusion of Bazooka (the Par3 protein) defines the position zonula adherens (ZA), which demarcates and lateral membrane allows cells to assemble into sheets. Here, we show that small GTPase Rap1, its effector Canoe (Cno) Cdc42 kinase Mushroom bodies tiny (Mbt), converge in regulating morphogenesis by coupling stabilization junction (AJ) protein E-Cadherin retention at ZA. Furthermore, our results localization Cno Mbt ZA is interdependent, indicating...
Abstract VPS33B and VIPAR comprise the two known components of recently christened class C Homologues in Endosome–Vesicle Interaction ( CHEVI ) complex, thought to act as a tethering complex endosomal trafficking distinct from HOPS CORVET complexes mammalian cells. A recent paper The Journal Pathology further explores role biogenesis α‐granules megakaryocytes, identifying novel interactors this complex: α‐tubulin SEC22B, demonstrating that expression is required for localization SEC22B...
Single nucelotide polymorphisms (SNPs) at the
Abstract The epithelial Zonula adherens (ZA) is a main adhesion compartment that enables organogenesis by allowing cells to assemble into sheets. How ZA assembly regulated during cell morphogenesis not fully understood. We show morphogenesis, the function of small GTPase Rap1 and F-actin binding protein AF6/Cno are both linked P21-activated kinase Pak4/Mbt. find Mbt regulate each other’s localization at cooperate in promoting ECadherin stabilization. During this process Cno regulates...
Abstract Keratinocyte cornification and epidermal barrier formation are tightly controlled processes, which require complete degradation of intracellular organelles, including removal keratinocyte nuclei. nuclear destruction requires Akt1-dependent phosphorylation the lamina protein, Lamin A/C, essential for integrity. However, molecular mechanisms that result in their regulation not well defined. Post-confluent cultures rat keratinocytes (REKs) undergo spontaneous differentiation, allowing...