A5075476177- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Congenital heart defects research
- Genetic and Kidney Cyst Diseases
- Hippo pathway signaling and YAP/TAZ
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Fluorescence Microscopy Techniques
- Congenital Diaphragmatic Hernia Studies
- Pulmonary Hypertension Research and Treatments
- Cardiovascular Conditions and Treatments
- Autophagy in Disease and Therapy
- Developmental Biology and Gene Regulation
- Congenital Ear and Nasal Anomalies
- Pancreatic function and diabetes
- Cephalopods and Marine Biology
- Coronary Artery Anomalies
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Physiological and biochemical adaptations
- Lipid metabolism and biosynthesis
- Axon Guidance and Neuronal Signaling
- Congenital gastrointestinal and neural anomalies
- Photoacoustic and Ultrasonic Imaging
- Cellular transport and secretion
- Renal and related cancers
Centre National de la Recherche Scientifique
2010-2025
École Polytechnique
2021-2025
Inserm
2021-2025
Max Delbrück Center
2018
Institut de Biologie Paris-Seine
2016
Laboratoire de Biologie du Développement
2010-2016
Sorbonne Université
2013-2016
Ludwig-Maximilians-Universität München
2013
University College London
2010
Abstract Cell migrations are crucial for embryonic development, wound healing, the immune response, as well cancer progression. During mesenchymal cell migration, Rac1-WAVE-Arp2/3 signalling pathway induces branched actin polymerisation, which protrudes membrane and allows migration. Fine-tuning activity of modulates protrusion lifetime migration persistence. Recently, NHSL1, a novel interactor Scar/WAVE complex has been identified negative regulator in vitro. We here analysed its function...
Rationale: Cardiac neural crest cells (NCs) contribute to heart morphogenesis by giving rise a variety of cell types from mesenchyme the outflow tract, ventricular septum, and semilunar valves neurons cardiac ganglia smooth muscles great arteries. Failure in NC development results septation defects commonly observed congenital diseases. NCs derive vagal tube, which also gives enteric that colonize gut; however, so far, molecular mechanisms segregating these 2 populations driving migration...
Mapping red blood cells (RBCs) flow and oxygenation is of key importance for analyzing brain tissue physiology. Current microscopy methods are limited either in sensitivity or spatio-temporal resolution. In this work, we introduce a novel approach based on label-free third-order sum-frequency generation (TSFG) third-harmonic (THG) contrasts. First, propose experimental scheme color TSFG microscopy, which provides simultaneous measurements at several wavelengths encompassing the Soret...
DiGeorge syndrome (DGS) is a congenital disease causing cardiac outflow tract anomalies, craniofacial dysmorphogenesis, thymus hypoplasia, and mental disorders. It results from defective development of neural crest cells (NC) that colonize the pharyngeal arches contribute to lower jaw, neck heart tissues. Although TBX1 has been identified as main gene accounting for defects observed in human patients mouse models, molecular mechanisms underlying DGS etiology are poorly identified. The recent...
A complex interplay of intrinsic factors and extrinsic signalling pathways controls both cell lineage commitment maintenance identity. Loss defined cellular states is the cause many different cancers, including pancreatic cancer. Recent findings suggest a clinical role for conserved SLIT/ROBO pathway in However, whilst this has been extensively studied processes, Slit Robo genes pancreas identity plasticity not established yet. Here, we identify Slit/Robo as key regulator progenitor We find...
Abstract Within the embryonic lung, intrinsic nerve ganglia, which innervate airway smooth muscle, are required for normal lung development and function. We studied of neural crest‐derived neurons within mouse by crossing Wnt1‐Cre mice with R26R‐EYFP reporter to generate double transgenic that express yellow fluorescent protein (YFP) in all crest cells (NCCs) their derivatives. In addition utilizing conventional immunohistochemistry on frozen sections, complex organization innervation was...
Centriolar satellites are high-order assemblies, scaffolded by the protein PCM1, that gravitate as particles around centrosome and play pivotal roles in fundamental cellular processes notably ciliogenesis autophagy. Despite stringent control mechanisms involving phosphorylation ubiquitination, landscape of post-translational modifications shaping these structures remains elusive. Here, we report necrosulfonamide (NSA), a small molecule known for binding inactivating effector cell death...
Polarization-resolved nonlinear optical microscopy can provide label-free images of anisotropic biomaterials. Existing techniques are often limited by their complexity, chromaticity, or slowness. In this article, we propose a simple implementation based on the passive splitting excitation beam into slightly delayed orthogonally polarized pulse trains. Label-free second- and third-harmonic signals corresponding to each polarization state separated time-resolved photon counting, resulting in...
The DiGeorge/22q11-deletion syndrome (22q11DS), also known as velocardiofacial syndrome, is a congenital disease causing numerous structural and behavioral disorders, including cardiac outflow tract anomalies, craniofacial dysmorphogenesis, parathyroid thymus hypoplasia, mental disorders. It results from unique chromosomal microdeletion on the 22q11.2 region in which transcriptional activator TBX1 decisive for occurrence of disease. During embryogenesis, Tbx1 required patterning pharyngeal...
Summary Cell migrations are crucial for embryonic development, wound healing, the immune response, as well cancer progression. During mesenchymal cell migration, Rac1-WAVE-Arp2/3 signalling pathway induces branched actin polymerisation, which protrudes membrane and allows migration. Fine-tuning activity of modulates protrusion lifetime migration persistence. Recently, NHSL1, a novel interactor Scar/WAVE complex has been identified negative regulator in vitro . We here analysed its function...
Morphogenesis involves many cell movements to organize cells into tissues and organs. For proper development, all these need be tightly coordinated, accumulating evidence suggests this is achieved, at least in part, through mechanical interactions. Testing the embryo requires direct physical perturbations. Laser ablations are an increasingly used option that allows relieving constraints or physically isolating two populations from each other. However, performed with ultraviolet (UV) laser,...
Morphogenesis involves many cell movements to organize cells into tissues and organs. For proper development, all these need be tightly coordinated, accumulating evidence suggests this is achieved, at least in part, through mechanical interactions. Testing the embryo requires direct physical perturbations. Laser ablations are an increasingly used option that allows relieving constraints or physically isolating two populations from each other. However, performed with ultraviolet (UV) laser,...
SUMMARY Centriolar satellites are high-order assemblies, scaffolded by the protein PCM1, that gravitate as particles around centrosome and play pivotal roles in fundamental cellular processes notably ciliogenesis autophagy. Despite stringent control mechanisms involving phosphorylation ubiquitination, landscape of post-translational modifications shaping these structures remains elusive. Here, we report necrosulfonamide (NSA), a small molecule known for binding inactivating effector cell...
We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several coherent signals produced by two synchronized femtosecond pulse trains. demonstrate that it can be used to obtain red blood cell (RBC)-specific label-free contrast in live zebrafish and is promising tool for probing RBC oxygenation.
Cilia protrude from the cell surface and play critical roles in in-tracellular signaling, environmental sensing development. Actin-dependent contractility intracellular trafficking are both required for ciliogenesis, but little is known about how these processes coordinated. Here, we identified a Rac1-and Rab35-binding protein with truncated BAR domain that named MiniBAR (aka KIAA0355/GARRE) which plays key role ciliogenesis. colocalizes Rac1 Rab35 at plasma membrane on vesicles traffick-ing...
Summary Morphogenesis, wound healing and some cancer metastases depend upon migration of cell collectives that need to be guided their destination as well coordinated with other movements. During zebrafish gastrulation, extension the embryonic axis is led by mesendodermal polster migrates towards animal pole, followed axial mesoderm undergoes convergence extension. We here investigate how cells are pole. Using a combination precise laser ablations, advanced transplants functional in silico...