A5007261809- Marine Ecology and Invasive Species
- Subterranean biodiversity and taxonomy
- Ichthyology and Marine Biology
- Marine Biology and Ecology Research
- Parasite Biology and Host Interactions
- Olfactory and Sensory Function Studies
- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- Fish Biology and Ecology Studies
- Aquatic life and conservation
- Neuroinflammation and Neurodegeneration Mechanisms
- Spider Taxonomy and Behavior Studies
- Venomous Animal Envenomation and Studies
- Neurobiology and Insect Physiology Research
- Microtubule and mitosis dynamics
- Genetic diversity and population structure
- Marine Sponges and Natural Products
- Hippo pathway signaling and YAP/TAZ
- Chromosomal and Genetic Variations
- Hedgehog Signaling Pathway Studies
- Cellular Mechanics and Interactions
- Physiological and biochemical adaptations
- Axon Guidance and Neuronal Signaling
Institut des Neurosciences Paris-Saclay
2017-2025
Centre National de la Recherche Scientifique
2017-2025
Université Paris-Saclay
2017-2023
CEA Paris-Saclay
2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2023
University of Valparaíso
2022
Université Paris-Sud
2017-2019
Valparaiso University
2014
Abstract Cell migrations are crucial for embryonic development, wound healing, the immune response, as well cancer progression. During mesenchymal cell migration, Rac1-WAVE-Arp2/3 signalling pathway induces branched actin polymerisation, which protrudes membrane and allows migration. Fine-tuning activity of modulates protrusion lifetime migration persistence. Recently, NHSL1, a novel interactor Scar/WAVE complex has been identified negative regulator in vitro. We here analysed its function...
The fish Astyanax mexicanus comes in two forms: the normal surface-dwelling and blind depigmented cave-adapted morphs. Comparing development of their basal forebrain, we found quantitative differences numbers cells specific clusters for six out nine studied neuropeptidergic cell types. Investigating origins these differences, showed that early Shh Fgf signaling impact on NPY Hypocretin clusters, via effect Lhx7 Lhx9 transcription factors, respectively. Finally, demonstrated such...
ABSTRACT Developmental evolution and diversification of morphology can arise through changes in the regulation gene expression or protein-coding sequence. To unravel mechanisms underlying early developmental cavefish species Astyanax mexicanus, we compared transcriptomes surface-dwelling blind cave-adapted morphs at end gastrulation. Twenty percent transcriptome was differentially expressed. Allelic ratios cave X surface hybrids showed that cis-regulatory are quasi-exclusive contributors to...
Sequential developmental events, starting from the moment of fertilization, are crucial for acquisition animal body plan. Subtle modifications in such early events likely to have major impacts later morphogenesis, bringing along morphological diversification. Here, comparing blind cave and surface morphotypes Astyanax mexicanus fish, we found heterochronies during gastrulation that produce organizer axial mesoderm tissues with different properties (including differences expression dkk1b) may...
ABSTRACT The vertebrate retinas originate from a specific anlage in the anterior neural plate called eye field. Its identity is conferred by set of ‘eye transcription factors’, whose combinatorial expression has been overlooked. Here, we use dimorphic teleost Astyanax mexicanus, which develops proper eyes wild type and smaller colobomatous blind cavefish embryos, to unravel molecular anatomy field its variations within species. Using series markers (rx3, pax6a, cxcr4b, zic1, lhx2, emx3...
The peripheral olfactory sensory system arises from morphologically identifiable structures called placodes. Placodes are relatively late developing structures, evident only well after the initiation of somitogenesis. generally described as being induced ectoderm suggesting that their development is separate coordinated cell movements generating central nervous system.With advent modern techniques it possible to follow neurectoderm giving rise anterior neural tube, including optic cup with...
In the vertebrate olfactory tract new neurons are continuously produced throughout life. It is widely believed that neurogenesis contributes to learning and memory can be regulated by immune signaling molecules. Proteins originally identified in system have subsequently been localized developing adult nervous system. Previously, we shown imprinting, a specific type of long-term memory, correlated with transcriptional response organs include up-regulation genes associated To better understand...
Animals in many phyla are adapted to and thrive the constant darkness of subterranean environments. To do so, cave animals have presumably evolved mechano- chemosensory compensations loss vision, as is case for blind characiform cavefish, Astyanax mexicanus. Here, we systematically assessed olfactory capacities cavefish surface fish this species lab well wild, five different caves northeastern Mexico, using an setup specially developed test record responses during fieldwork. Overall showed...
The olfactory epithelia arise from morphologically identifiable structures called placodes. Sensory placodes are generally described as being induced the ectoderm suggesting that their development is separate coordinated cell movements generating central nervous system. Previously, we have shown a large field of cells bordering telencephalic precursors in neural plate, and movements, not division, underlie placode morphogenesis. Subsequently by image analysis, were tracked they moved...
The fish species Astyanax mexicanus with its sighted and blind eco-morphotypes has become an original model to challenge vertebrate developmental evolution. Recently, we demonstrated that phenotypic evolution can be impacted by early events starting from the production of oocytes in ovaries. A. offers amenable test influence maternal determinants on cell fate decisions during development, yet mechanisms which information contained eggs is translated into specific programs remain obscure due...
ABSTRACT Changes in gene expression regulation during development are considered the main drivers of morphological evolution and diversification. Here, we analysed embryonic transcriptomes surface-dwelling blind cave-adapted morphs species Astyanax mexicanus their reciprocal F1 hybrids at tailbud stage. Comparing parents allelic ratios hybrids, found that ∼20% transcriptome is differentially expressed cis -regulatory changes contributors to variations early developmental two morphs. We...
Abstract The fish species Astyanax mexicanus with its sighted and blind eco-morphotypes has become an original model to challenge vertebrate developmental evolution. Recently, we demonstrated that phenotypic evolution can be impacted by early events starting from the production of oocytes in ovaries. A. offers amenable test influence maternal effect on cell fate decisions during development, yet mechanisms which information contained eggs is translated into specific programs remain obscure...
Monoaminergic systems are conserved in vertebrates, yet they present variations neuroanatomy, genetic components and functions across species. MonoAmine Oxidase, or MAO, is the enzyme responsible for monoamine degradation. While mammals possess two genes, MAO-A MAO-B, fish one single mao gene. To study function of MAO homeostasis on brain development physiology, here we have generated a knockout line Astyanax mexicanus (surface fish), by CRISPR/Cas9 technology. Homozygote larvae died at 13...
Abstract The vertebrate retinas originate from a specific anlage in the anterior neural plate called eyefield. eyefield shares its border with prospective telencephalon and is contact ventrally posteriorly hypothalamic diencephalic precursors. Eyefield identity conferred by set of “eye transcription factors”, whose combinatorial expression has not been precisely characterized. Here, we use dimorphic teleost species Astyanax mexicanus , which develops proper eyes wild type smaller...
Sex chromosomes are generally derived from a pair of classical type-A chromosomes, and relatively few alternative models have been proposed up to now. B (Bs) supernumerary dispensable with non-Mendelian inheritance found in many plant animal species, that often considered as selfish genetic elements behave genome parasites. The observation some species Bs can be either restricted or predominant one sex raised the interesting hypothesis could play role determination. characterization putative...
Abstract Sequential developmental events, starting from the moment of fertilization, are crucial for acquisition animal body plan. Subtle modifications in such early events likely to have a major impact later morphogenesis, bringing along morphological diversification. Here, comparing blind cave and surface morphotypes Astyanax mexicanus fish, we found heterochronies during gastrulation, producing organizer axial mesoderm tissues with different properties, including differences expression...