A5064102620- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Microbial Community Ecology and Physiology
- Clostridium difficile and Clostridium perfringens research
- Genetic diversity and population structure
- Identification and Quantification in Food
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Parasite Biology and Host Interactions
- Environmental DNA in Biodiversity Studies
- Reproductive biology and impacts on aquatic species
- Chromosomal and Genetic Variations
- Fish biology, ecology, and behavior
- Neutropenia and Cancer Infections
- Mycobacterium research and diagnosis
- Animal Nutrition and Physiology
- Plant Virus Research Studies
- Cancer therapeutics and mechanisms
- Genetics, Bioinformatics, and Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Genetic and phenotypic traits in livestock
- Enterobacteriaceae and Cronobacter Research
- Fecal contamination and water quality
- Agriculture Sustainability and Environmental Impact
- Phytoplasmas and Hemiptera pathogens
- Subterranean biodiversity and taxonomy
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2023
Institut National de la Recherche Agronomique
2019
Institut National de la Recherche Agronomique d'Algérie
2019
Université Toulouse III - Paul Sabatier
2018
Université de Toulouse
2018
Accurate species phylogenies are a prerequisite for all evolutionary research. Teleosts the largest and most diversified group of extant vertebrates, but relationships among their three oldest lineages remain unresolved. On basis seven high-quality new genome assemblies in Elopomorpha (tarpons, eels), we revisited topology deepest branches teleost phylogeny using independent gene sequence chromosomal rearrangement phylogenomic approaches. These analyses converged to single scenario that...
The evolution of sex determination (SD) in teleosts is amazingly dynamic, as reflected by the variety different master sex-determining genes identified. Pangasiids are economically important catfishes South Asian countries, but little known about their SD system. Here, we generated novel genomic resources for 12 and characterized Based on a Pangasianodon hypophthalmus chromosome-scale genome assembly, identified an anti-Müllerian hormone receptor type Ⅱ gene (amhr2) duplication, which was...
Our study was designed to gain a better understanding of how different feeding patterns affect the dynamics gut microbiomes and microbe–host interactions. This research showed that timing solid food introduction is key component microbiota shaping in early developmental stages, though with lower impact on settled profiles older individuals.
Abstract Inspired by the production of reference data sets in Genome a Bottle project, we sequenced one Charolais heifer with different technologies: Illumina paired-end, Oxford Nanopore, Pacific Biosciences (HiFi and CLR), 10X Genomics linked-reads, Hi-C. In order to generate haplotypic assemblies, also both parents short reads. From these data, built two haplotyped trio high quality genomes consensus assembly, using up-to-date software packages. The assemblies obtained PacBio HiFi reaches...
Contaminated pork is a significant source of foodborne Salmonellosis. Pork contaminated at the slaughterhouse and intestinal content predominant
Abstract Background To study communities of micro-organisms taxonomically and functionally, metagenomic analyses are now often used. If there is no reference gene catalogue, a de novo approach required. Because genomes easier to interpret than contigs, the recovery metagenome-assembled (MAGs) by binning contigs from data has recently become common task for microbial studies. However, during this process, significant loss information between assembly contigs. This why it important produce...
Abstract Enterotoxigenic Escherichia coli (ETEC) is the aetiological agent of postweaning diarrhoea (PWD) in piglets. The SNPs located on Mucine 4 ( MUC4 ) and Fucosyltransferase 1 FUT1 genes have been associated with susceptibility to ETEC F4 F18, respectively. interplay between genotypes infection use amoxicillin modifying intestinal microbiota during a natural by multiresistant strains never investigated. aim this study was evaluate effects administration through different routes presence...
This study was conducted to test the ability of a carvacrol-based formulation (Phodé, France) decrease C. jejuni caecal load in inoculated broiler chickens and impact inoculation alone or combined with product, on microbiota.On day 1, were either fed control feed same diet supplemented product. On 21, carvacrol-supplemented half non-supplemented (108 CFU). Quantitative PCR used quantify chicken samples 16S rRNA gene sequencing carried out at 25, 31 35 days age. A significant 1.4 log observed...
Abstract RHO GTPases regulate essential functions such as the organization of actin cytoskeleton. The classic members cycle between an active GTP-bound and inactive GDP-bound conformation whereas atypical are predominantly GTP-bound. Besides their well-established role, RHOB RAC1, rapidly induced and/or activated by genotoxic stress contribute to DNA damage response. Here we used camptothecin, a selective topoisomerase I (TOP1) inhibitor that stabilizes TOP1 cleavage complexes (TOP1cc),...
ABSTRACT The evolution of sex determination (SD) mechanisms in teleost fishes is amazingly dynamic, as reflected by the variety different master sex-determining genes identified, even sometimes among closely related species. Pangasiids are a group economically important catfishes many South-Asian countries, but little known about their system. Here, we generated novel genomic resources for 12 Pangasiid species and provided first characterization SD Based on an Oxford Nanopore long-read...
Abstract Accurate species phylogenies are a prerequisite for evolutionary research. Teleosts by far the largest and most diversified group of extant vertebrates, but relationships among three oldest lineages teleosts remain unresolved. Based on seven high-quality new genome assemblies in Elopomorpha (tarpons, eels), we revisited topology deepest branches teleost phylogeny using independent gene sequence chromosomal rearrangement phylogenomic approaches. These analyses converged to single...
In third generation sequencing, the production of quality data requires selection molecules longer than ∼20 kbp, but size threshold most purification technologies is smaller this target. Here, we describe a technology operated in capillary with tunable range 3 to 40 kbp controlled by an electric field. We demonstrate that cut-off sharp, yield high, and throughput scalable. also provide analytical model actuation settings filter. The high molecular weight genomic DNA from melon Cucumis melo...
Abstract The transition from a milk-based diet to exclusive solid feeding deeply modifies microbiota-host crosstalk. Specifically, early ingestion of plant polysaccharides would be one the main nutritional components drive host-microbiota-interaction. To capture effects early-life nutrition (starch vs rapidly fermentable fiber) on holobiont development, we investigated hand gut bacteriome and metabolome other transcriptome two host tissues. Rabbit model was used study post-natal...
Sex chromosomes are generally derived from a pair of classical type-A chromosomes, and relatively few alternative models have been proposed up to now. B (Bs) supernumerary dispensable with non-Mendelian inheritance found in many plant animal species, that often considered as selfish genetic elements behave genome parasites. The observation some species Bs can be either restricted or predominant one sex raised the interesting hypothesis could play role determination. characterization putative...
DNA size fractionation is an essential tool in molecular biology and used to isolate targets a mixture characterized by broad molecular-weight distribution. Microfluidics was thought provide the opportunity create devices capable of enhancing speeding up classical processes. However, this conjecture met limited success due low mass or volume throughput these technologies. We describe μLAF (μ-laboratory for fractionation) technology selection based on stacking molecules films ∼100 μm...