A5034436415- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Lung Cancer Treatments and Mutations
- Cell Adhesion Molecules Research
- Receptor Mechanisms and Signaling
- Cancer therapeutics and mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Cancer Genomics and Diagnostics
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- Virus-based gene therapy research
- Neutropenia and Cancer Infections
- Cancer-related gene regulation
- PI3K/AKT/mTOR signaling in cancer
- Colorectal Cancer Treatments and Studies
- Phagocytosis and Immune Regulation
- HIV Research and Treatment
- Cellular transport and secretion
- Retinal Development and Disorders
- Cell Image Analysis Techniques
- Melanoma and MAPK Pathways
- S100 Proteins and Annexins
- Immune Cell Function and Interaction
- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
Inserm
2014-2024
Université Toulouse III - Paul Sabatier
2014-2024
Université de Toulouse
2017-2024
Centre de Recherche en Cancérologie de Toulouse
2014-2024
Centre National de la Recherche Scientifique
2018-2024
Macrophages are essential for HIV-1 pathogenesis and represent major viral reservoirs. Therefore, it is critical to understand macrophage infection, especially in tissue macrophages, which widely infected vivo, but poorly permissive cell-free infection. Although cell-to-cell transmission of a determinant mode infection how transfers toward macrophages remains elusive. Here, we demonstrate that fusion CD4+ T lymphocytes with human leads their efficient productive Importantly, several...
Abstract Drug-tolerance has emerged as one of the major non-genetic adaptive processes driving resistance to targeted therapy (TT) in non-small cell lung cancer (NSCLC). However, kinetics and sequence molecular events governing this response remain poorly understood. Here, we combine real-time monitoring cell-cycle dynamics single-cell RNA sequencing a broad panel oncogenic addiction such EGFR-, ALK-, BRAF- KRAS-mutant NSCLC, treated with their corresponding TT. We identify common path drug...
ABSTRACT The human Ras superfamily of small GTPases controls essential cellular processes such as gene expression and cell proliferation. As their deregulation is widely associated with cancer, regulatory proteins have become increasingly attractive for the development novel therapeutics. Classical methods to monitor GTPase activation include pulldown assays that limit analysis GTP-bound form from lysates. Alternatively, live-cell FRET biosensors may be used study dynamics in response...
Purpose: The Epstein–Barr virus (EBV)-associated cancer nasopharyngeal carcinoma (NPC) is rare in Europe and North America but a real public health problem some regions of the world, such as southern Asia, Africa, for Inuit populations. Due to anatomy location nasopharynx, surgery rarely used treat primary NPC cancers. Treatment by radiotherapy, combined or not with chemotherapy, are efficient tumors often do protect against fatal relapses metastases. Methods: Search new therapeutic...
Ectopic Fas-ligand (FasL) expression in tumor cells is responsible for both escape through counterattack of Fas-positive infiltrating lymphocytes and rejection though inflammatory immune responses. We have previously shown that RhoA GTPase its effector ROCK negatively control FasL membrane murine melanoma B16F10 cells. In this study, we found treatment with the inhibitor H1152 reduced development vivo overexpression. Although did not reduce growth vitro, pretreatment delayed appearance,...
Cell-cell fusion is an evolutionarily conserved process that essential for many functions, including fertilisation and the formation of placenta, muscle osteoclasts, multinucleated cells are unique in their ability to resorb bone. The mechanisms osteoclast multinucleation involve dynamic interactions between actin cytoskeleton plasma membrane still poorly characterized. Here, we found moesin, a cytoskeletal linker protein member Ezrin/Radixin/Moesin (ERM) family, activated during maturation...
Small GTPases are highly regulated proteins that control essential signaling pathways through the activity of their effector proteins. Among RHOA subfamily, RHOB regulates peculiar functions could be associated with endocytic trafficking Here, we used an optimized assay based on tripartite split-GFP complementation to localize GTPase-effector complexes high-resolution. The detection interaction Rhotekin Rho binding domain (RBD) specifically recognizes active GTP-bound GTPase, is performed in...
Abstract RHO GTPases regulate essential functions such as the organization of actin cytoskeleton. The classic members cycle between an active GTP-bound and inactive GDP-bound conformation whereas atypical are predominantly GTP-bound. Besides their well-established role, RHOB RAC1, rapidly induced and/or activated by genotoxic stress contribute to DNA damage response. Here we used camptothecin, a selective topoisomerase I (TOP1) inhibitor that stabilizes TOP1 cleavage complexes (TOP1cc),...
CD70 is a costimulatory molecule member of the Tumor Necrosis Factor family that expressed on activated immune cells. Its ectopic expression has been described in several types cancer cells including lymphomas, renal cell carcinomas and glioblastomas. We have recently its part tumor from vast majority melanoma biopsies human lines, found decreased over time as disease progressed. Here, we show RhoA, BRAF Mitogen Activating Protein Kinase pathways are involved positive transcriptional...
Determining the cellular level of activated form RhoGTPases is key importance to understand their regulatory functions in cell physiopathology. We previously reported scFvC1, that selectively bind GTP-bound RhoA, RhoB and RhoC. In this present study we generate, by molecular evolution, a new phage library isolate scFvs displaying high affinity selectivity RhoA RhoB. Using display maturation against GTP-locked mutant RhoAL63, isolated active conformation Kd values at nanomolar range, which...
ABSTRACT Drug-tolerant “dormant” cells (DTC) have emerged as one of the major non-genetic mechanisms driving resistance to targeted therapy in lung cancer, although sequence events leading entry and exit from dormancy remain poorly described. Here, we performed real-time monitoring cell cycle dynamics during adaptive response Epidermal Growth Factor Receptor tyrosine kinase inhibitors (EGFR-TKi) a panel EGFR-mutated cancer lines. We identified rare population S/G2 cycling (referred early...
The selective down-regulation of activated intracellular proteins is a key challenge in cell biology. RHO small GTPases switch between GDPbound and GTP-bound state that drives downstream signaling. To date, no tool available to study endogenous induced conformational changes live cells. Here, we set up an original cell-based screen selectively degrade the RHOB-GTP fraction using nanobodies functionalized with F-box domain. We identified one intrabody shows targeting mediated by interactions...
Abstract Purpose: Drug-tolerant “dormant” cells (DTC) have emerged as one of the major non-genetic mechanisms driving resistance to targeted therapy in lung cancer, although sequence events leading entry and exit from dormancy remain poorly described. Here, we provide a step-by-step phenotypic molecular characterization different processes involved during adaptive response osimertinib using several EGFR-mutated cancer models. This strategy led identification common vulnerability...
Strategies based on intracellular expression of artificial binding domains present several advantages over manipulating nucleic acid or the use small molecule inhibitors. Intracellularly-functional nanobodies can be considered as promising macrodrugs to study key signaling pathways by interfering with protein-protein interactions. With aim studying RAS-related GTPase RHOA family, we previously isolated, from a synthetic phage display library, selective towards GTP-bound conformation...