A5028074922- Zebrafish Biomedical Research Applications
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- RNA modifications and cancer
- Photoreceptor and optogenetics research
- Microstructure and mechanical properties
- Aluminum Alloys Composites Properties
- Metallurgy and Material Forming
- Electromagnetic Simulation and Numerical Methods
- Metallurgy and Material Science
- Neural dynamics and brain function
- Aquaculture disease management and microbiota
- Advanced Fluorescence Microscopy Techniques
- Cellular transport and secretion
- Electromagnetic Scattering and Analysis
- Neuroscience and Neuropharmacology Research
- Aluminum Alloy Microstructure Properties
- Ion channel regulation and function
- Protein Kinase Regulation and GTPase Signaling
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
National Institute of Genetics
2009-2019
The Graduate University for Advanced Studies, SOKENDAI
2011-2019
Hirosaki University
1997-2015
University of California, San Francisco
2004-2014
University of California, Berkeley
2012
Sony Computer Science Laboratories
2010-2011
Nagahama Institute of Bio-Science and Technology
2010
Renesas Electronics (United States)
2007
Akita University
1994-2005
Iwate University
2001
The genomic guanine and cytosine (G + C) content of eubacteria is related to their phylogeny. G C various parts the genome (protein genes, stable RNA spacers) reveals a positive linear correlation with DNA. However, plotted slopes differ among or first, second, third positions codons depending on functional importance. Facts suggest that biased mutation pressure, called A X T/G has affected whole DNA during evolution so as determine in given bacterium. role pressure diversification bacterial...
The visual system converts the distribution and wavelengths of photons entering eye into patterns neuronal activity, which then drive motor endocrine behavioral responses. gene products important for processing by a living behaving vertebrate animal have not been identified in an unbiased fashion. Likewise, genes that affect development nervous to shape function later life are largely unknown. Here we set out close this gap our understanding using forward genetic approach zebrafish. Moving...
UGA is a nonsense or termination (opal) codon throughout prokaryotes and eukaryotes. However, mitochondria use not only UGG but also as tryptophan codon. Here, we show that codes for in Mycoplasma capricolum, wall-less bacterium having genome 20-25% the size of Escherichia coli genome. This conclusion based on following evidence. First, nucleotide sequence S3 L16 ribosomal protein genes from M. capricolum includes codons reading frames; they appear at positions corresponding to E. L16....
Animal behaviors are generated by well-coordinated activation of neural circuits. In zebrafish, embryos start to show spontaneous muscle contractions at 17 19 h postfertilization. To visualize how motor circuits in the spinal cord activated during this behavior, we developed GCaMP-HS (GCaMP- yper s ensitive), an improved version genetically encoded calcium indicator GCaMP, and created transgenic zebrafish carrying gene downstream Gal4-recognition sequence, UAS (upstream sequence). Then...
Abstract Background We have developed genetic methods in zebrafish by using the Tol2 transposable element; namely, transgenesis, gene trapping, enhancer trapping and Gal4FF-UAS system. Gene trap constructs contain a splice acceptor GFP or Gal4FF (a modified version of yeast Gal4 transcription activator) gene, hsp70l promoter gene. By performing screens these constructs, we generated transgenic that express specific cells, tissues organs. expression is visualized creating double fish carrying...
Abstract Brain activity and connectivity alter drastically during epileptic seizures. The brain networks shift from a balanced resting state to hyperactive hypersynchronous state. It is, however, less clear which mechanisms underlie the transitions. By studying neural glial in zebrafish models of seizures, we observe striking differences between these networks. During preictal period, neurons display small increase synchronous only locally, while gap-junction-coupled network was highly...
Fear conditioning is a form of learning essential for animal survival and used as behavioral paradigm to study the mechanisms memory. In mammals, amygdala plays crucial role in fear conditioning. teleost, medial zone dorsal telencephalon (Dm) has been postulated be homolog mammalian by anatomical ablation studies, showing conditioned avoidance response. However, neuronal populations required response via Dm have not functionally or genetically defined. We aimed identify population through...
The visual system plays a major role in food/prey recognition diurnal animals, and food intake is regulated by the hypothalamus. However, whether how information about prey conveyed to hypothalamic feeding centre largely unknown. Here we perform real-time imaging of neuronal activity freely behaving or constrained zebrafish larvae demonstrate that prey-like stimuli activate centre. Furthermore, identify detector neurons pretectal area project Ablation pretectum completely abolishes capture...
Sprouting angiogenesis is a well-coordinated process controlled by multiple extracellular inputs, including vascular endothelial growth factor (VEGF). However, little known about when and how individual cell (EC) responds to angiogenic inputs in vivo. Here, we visualized Ca(2+) dynamics zebrafish found that intracellular oscillations occurred ECs exhibiting behavior. depended upon VEGF receptor-2 (Vegfr2) Vegfr3 budding from the dorsal aorta (DA) posterior cardinal vein, respectively. Thus,...
We updated the tRNADB-CE by analyzing 939 complete and 1301 draft genomes of prokaryotes eukaryotes, 171 virus genomes, 121 chloroplast approximately 230 million sequences obtained metagenome analyses 210 environmental samples. The 287 102 tRNA genes in total, thus two times compiled previously, are compiled, which sequence information, clover-leaf structure results similarity oligonucleotide-pattern search can be browsed. In order to pool collective knowledge with help from any experts...
The inositol 1,4,5-trisphosphate (IP 3 ) receptor is a calcium ion channel involved in the release of free Ca 2+ from intracellular stores. For analysis role IP -induced (IICR) on patterning embryonic body, monoclonal antibodies that inhibit IICR were produced. Injection these blocking into ventral part early Xenopus embryos induced modest dorsal differentiation. A close correlation between potencies and ectopic axis induction frequency suggests an active -Ca signal may participate modulation
Calcium signaling is known to be associated with cytokinesis; however, the detailed spatio-temporal pattern of calcium dynamics has remained unclear. We have studied changes intracellular free in cleavage-stage Xenopus embryos using fluorescent indicator dyes, mainly Green-1. Cleavage formation was followed by transients that localized cleavage furrows and propagated along as waves. The at were observed each furrow least until blastula stage. velocity waves first approximately 3 microns/s,...
Mutations in the human CACNA1F gene cause incomplete congenital stationary night blindness type 2 (CSNB2), a non-progressive, clinically heterogeneous retinal disorder. However, molecular mechanisms underlying CSNB2 have not been fully explored. Here, we describe positional cloning of blind zebrafish mutant, wait until dark (wud), which encodes homolog CACNA1F. We identified two cacna1f paralogs and showed that cacna1fa transcript (the mutated wud) is expressed exclusively photoreceptor...