A5048312470- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- RNA regulation and disease
- Genetics, Aging, and Longevity in Model Organisms
- Platelet Disorders and Treatments
- Inflammatory Biomarkers in Disease Prognosis
- Epigenetics and DNA Methylation
- COVID-19 Clinical Research Studies
- Telomeres, Telomerase, and Senescence
- Circular RNAs in diseases
- Long-Term Effects of COVID-19
- Single-cell and spatial transcriptomics
Medical University of Vienna
2022
Institute of Molecular Biotechnology
2019-2020
BOKU University
2019-2020
Modifications of ribosomal RNA expand the nucleotide repertoire and thereby contribute to ribosome heterogeneity translational regulation gene expression. One particular m5C modification 25S RNA, which is introduced by Rcm1p, was previously shown modulate stress responses lifespan in yeast other small organisms. Here, we report that NSUN5 functional orthologue introducing m5C3782 into human m5C3438 mouse 28S RNA. Haploinsufficiency fibroblasts from William Beuren syndrome patients causes...
The plasma levels of tissue-specific microRNAs can be used as diagnostic, disease severity and prognostic biomarkers for chronic acute diseases drug-induced injury. Thereby, the combination diverse into biomarker signatures using multivariate statistics seems especially powerful from perspective tissue condition specific microRNA shedding plasma. Although next-generation sequencing (NGS) technology enables one to analyse circulating on a genome-scale level, it suffers potential biases (e.g.,...
Our knowledge about the repertoire of ribosomal RNA modifications and enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 depositing m 5 C at position C2381 on 26S rRNA in Caenorhabditis elegans . Here, show NSUN-1 writing second known C2982. Depletion nsun-1 or nsun-5 improved thermotolerance slightly increased locomotion midlife, however, only soma-specific knockdown extended lifespan. Moreover, reduced body size impaired fecundity,...
Blood-derived microRNA signatures have emerged as powerful biomarkers for predicting and diagnosing cardiovascular disease, cancer, metabolic disorders. Platelets platelet-derived microvesicles are a major source of microRNAs. We previously shown that the inappropriate anticoagulation storage blood samples causes substantial platelet activation is associated with release platelet-stored molecules into plasma. However, it currently unclear if circulating levels affected by artificial due to...
Dysregulation of platelet function is causally connected to thrombus formation and cardiovascular diseases. Therefore, assessing reactivity crucial. However, current tests come with pitfalls, limiting clinical use. Plasma miRNA signatures have been suggested as novel biomarkers for predicting/diagnosing diseases monitoring antiplatelet therapy. Here, we provide results from a comprehensive study on the feasibility using circulatory miRNAs surrogate markers activation. We performed small...
Abstract In the last decade cellular senescence, a hallmark of aging, has come into focus for pharmacologically targeting aging processes. Senolytics are one these interventive strategies that have advanced clinical trials, creating an unmet need minimally invasive biomarkers senescent cell load to identify patients at senotherapy. We created landscape miRNA and mRNA expression in five human types induced senescence in-vitro provide proof-of-principle evidence can track burden dynamically...
Abstract Extracellular vesicles (EVs) represent nanometer-sized, subcellular spheres, that are released from almost any cell type and carry a wide variety of biologically relevant cargo. In severe cases coronavirus disease 2019 (COVID-19) other states systemic pro-inflammatory activation, EVs, their cargo can serve as conveyors indicators for severity progression. This information may help distinguish individuals with less manifestation the patients who exhibit acute respiratory distress...
Platelets are the main effectors of primary hemostasis but also cause thrombosis in pathological conditions. Antiplatelet drugs cornerstone for prevention adverse cardiovascular events. Monitoring extent platelet inhibition is essential. Currently available function tests come with constraints, limiting use antiplatelet drug development as well clinical routine. With this study, we aim to investigate whether plasma miRNAs might be suitable biomarkers monitoring treatment. Platelet-poor was...
Platelets are involved in a variety of diseases, making their adequate functional assessment is essential. However, due to easily activatable nature this has some methodological pitfalls. Therefore, the availability stable, measurable surrogate markers would be beneficial. In regard, evidence suggests that certain microRNAs (miRNAs) circulating plasma might useful. We aimed corroborate suitability by analyzing samples obtained randomized controlled trial, which assessed effects periodontal...
Abstract Our knowledge about the repertoire of ribosomal RNA modifications and enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 depositing m 5 C at position C2381 on 26S rRNA in Caenorhabditis elegans . Here, show NSUN-1 writing second known C2982. Depletion nsun-1 or nsun-5 improved locomotion midlife resistance against heat stress, however, only soma-specific knockdown extended lifespan. Moreover, reduced body size impaired fecundity,...