A5006541973- Photosynthetic Processes and Mechanisms
- DNA Repair Mechanisms
- Fungal and yeast genetics research
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- T-cell and B-cell Immunology
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- Circular RNAs in diseases
- Mass Spectrometry Techniques and Applications
- thermodynamics and calorimetric analyses
- Protein Structure and Dynamics
- Mitochondrial Function and Pathology
- Analytical Chemistry and Chromatography
- Hemoglobin structure and function
- Carcinogens and Genotoxicity Assessment
- Immune cells in cancer
- Advanced Statistical Methods and Models
- Listeria monocytogenes in Food Safety
- Complement system in diseases
- Biochemical and Structural Characterization
University of Edinburgh
2002-2025
The Queen's Medical Research Institute
2024
University of Aberdeen
2016-2020
Institute of Genetics and Cancer
2017-2019
Institute of Medical Sciences
2019
Midwestern University
2017
University of Tasmania
2012-2015
Centre for Inflammation Research
2002
University of Nottingham
1997
UCL Australia
1994
Graphical AbstractFirst-generation, bare-metal stents successfully open and support occluded vessels but are prone to in-stent restenosis caused by excessive vascular smooth muscle cell proliferation. Drug-eluting overcome these drawbacks coating with anti-proliferative compounds, due the broad mechanism of action in blocking proliferation different types, they associated late-stent thrombosis delayed or impaired re-endothelialization. CCN5 protein-coated designed both block promote...
Abstract Objective A deficiency in a subcomponent of C1q can result increased susceptibility to autoimmune diseases such as systemic lupus erythematosus (SLE). The monocyte endocytic receptor CD91 is implicated the endocytosis apoptotic neutrophils via interactions with and calreticulin. In this clinical study, we studied binding leukocytes determined whether bound specifically calreticulin on cells undergoing apoptosis SLE. Methods Proximal antibody phage display, calreticulin‐transfected...
Summary Analysis of the N ‐ethyl‐ ‐nitrosourea ( ENU )‐induced repro42 mutation previously identified spermatogenesis associated 22 Spata22 ) as a gene required for meiotic progression and fertility in both male female mice, but its specific contribution to process was unclear. Here, we report on novel, null allele Gt confirm requirement germ cell development. Similar mutant histological mating analyses indicate that gametogenesis is profoundly affected Gt/Gt males females, resulting...
Abstract Changes in environmental temperature influence cellular processes and their dynamics, thus affect the life cycle of organisms that are unable to control cell/body temperature. Meiotic recombination is process essential for producing healthy haploid gametes by providing physical links (chiasmata) between homologous chromosomes guide accurate segregation. Additionally, meiotic recombination—initiated programmed DNA double-strand breaks (DSBs)—can generate genetic diversity and,...
Objective To compare the results of a computer programme based on Trigg's tracking system (TTS) identification basal body temperature (BBT) shift day from daily records BBT values (TTS transition day), with identified same using Sensiplan® symptothermal method natural family planning.Methods A was written to display readings for 364 menstrual cycles 51 women aged 24 35 years, obtained German Natural Family Planning (NFP) database. The TTS so each record then compared estimated by...
A technique has been developed for the direct analysis by visible spectrophotometry of yeast spots growing on agar plates. This allows rapid semi‐quantitative estimations cytochromes c , b and oxidase permits identification strains with impaired respiratory electron flow. Results screening 105 mutants are presented. There appears to be a correlation between exonic location mutation in COX1 level optically detectable enzyme. Mutations cytochrome bc 1 complex also affect expression can cause...
The ability to utilize different selectable markers for tagging or mutating multiple genes in Schizosaccharomyces pombe is hampered by the historical use of only two markers, ura4+ and kanMX6; latter conferring resistance antibiotic G418 (geneticin). More have been described recently, but introducing these into yeast cells often requires strain construction from scratch. To overcome this problem we other groups created transformation cassettes with flanking homologies kanMX6 which enable an...
Hop1 is a component of the meiosis-specific chromosome axis and belongs to evolutionarily conserved family HORMA domain proteins. its orthologs in higher eukaryotes are major factor promoting double-strand DNA break formation inter-homolog recombination. In budding yeast mammals, they also involved meiotic checkpoint kinase cascade monitoring completion repair. We used fission yeast, Schizosaccharomyces pombe, which lacks canonical synaptonemal complex test whether has role beyond supporting...
Abstract Meiotic recombination is essential for producing healthy gametes, and also generates genetic diversity. DNA double-strand break (DSB) formation the initiating step of meiotic recombination, producing, among other outcomes, crossovers between homologous chromosomes (homologs), which provide physical links to guide accurate chromosome segregation. The parameters influencing DSB position repair are thus crucial determinants reproductive success Using Schizosaccharomyces pombe , we show...
Analysis of a generalised growth equation shows that both the maximum rate microbial culture and duration lag phase are related to each other growth. Similar relationships apply expressions, such as logistic Gompertz models, special cases model. Moreover, same observed qualitatively in measurements Salmonella species. These results may allow characterisation with fewer parameters than is usually case imply likelihood fundamental physiological interdependence between rate, time References...
The behavior of enzyme-catalyzed reactions is not made clear to many students by the standard mathematical description enzyme kinetics. An enzyme-machine analogy described that has details Michaelis-Menten mechanism and associated kinetics more accessible with minimal use mathematics. Students taught using appear have fewer misconceptions than those a approach.
Conference Article| August 01 1991 MOA-stilbene: a new effector of the Qi site chloroplast cytochrome bf complex PETER R. RICH; RICH 1Glynn Research Institute, Bodmin, Cornwall PL30 4AU, United Kingdom Search for other works by this author on: This Site PubMed Google Scholar SALLY A. MADGWICK; MADGWICK SIMON BROWN; BROWN GEBHARD VON JAGOW; JAGOW *Gustav-Embden-Zentrum der Biologischen Chemie, Klinikum Johann Wolfgang Goethe-Universität, 6000 Frankfurt, Germany ULRICH BRANDT Biochem Soc Trans...
Ligand-binding assays, such as immunoassays, are usually analysed using standard curves based on the four-parameter and five-parameter logistic models. An estimate of uncertainty an analyte concentration obtained from is needed for confidence intervals or precision profiles. Using a numerical simulation approach, it shown that becomes significant at extremes range this affected significantly by steepness curve. We also provide expressions coefficient variation which profile can be obtained....