A5070165519- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- Nuclear Structure and Function
- Neuroscience and Neural Engineering
- Particle Accelerators and Free-Electron Lasers
- Force Microscopy Techniques and Applications
- Cardiac Fibrosis and Remodeling
- Laser Applications in Dentistry and Medicine
- Congenital heart defects research
- Cardiovascular Effects of Exercise
- Particle Detector Development and Performance
- 3D Printing in Biomedical Research
- Laser-Plasma Interactions and Diagnostics
- Particle accelerators and beam dynamics
- Genetic Neurodegenerative Diseases
- Gyrotron and Vacuum Electronics Research
- Genetics and Neurodevelopmental Disorders
- Oral health in cancer treatment
- Muscle Physiology and Disorders
- Photodynamic Therapy Research Studies
- Signaling Pathways in Disease
- Polymer Surface Interaction Studies
- Carbon Nanotubes in Composites
Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Legnaro
2021-2024
International Centre for Genetic Engineering and Biotechnology
2011-2022
Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali di Frascati
2018-2020
Institute of Molecular Medicine
2019
Istituto Nazionale di Fisica Nucleare
2018
University of Trieste
2013-2017
University of Colorado Denver
2013-2016
Istituto Nazionale di Fisica Nucleare, Sezione di Roma I
2016
Sapienza University of Rome
2016
Centro de Ingeniería Genética y Biotecnología
2015
Recent evidence indicates that a few human microRNAs (miRNAs), in particular hsa-miR-199a-3p and hsa-miR-590-3p, stimulate proliferation of cardiomyocytes and, once expressed the mouse heart using viral vectors, induce cardiac regeneration after myocardial infarction. Viral however, are not devoid safety issues more notably, drive expression encoded miRNAs for indefinite periods time, which might be desirable light therapeutic application.As an alternative to use we wanted assess efficacy...
Nanoscale manipulations of the extracellular microenvironment are increasingly attracting attention in tissue engineering. Here, combining microscopy, biological, and single-cell electrophysiological methodologies, we demonstrate that neonatal rat ventricular myocytes cultured on substrates multiwall carbon nanotubes interact with by forming tight contacts show increased viability proliferation. Furthermore, observed changes properties cardiomyocytes, suggesting able to promote cardiomyocyte...
Cardiomyocyte proliferation stops at birth when the heart is no longer exposed to maternal blood and, likewise, regulatory T cells (Tregs) that are expanded promote tolerance towards fetus. Here, we report a role of Tregs in promoting cardiomyocyte proliferation. Treg-conditioned medium promotes proliferation, similar serum from pregnant animals. Proliferative cardiomyocytes detected mothers, and Treg depletion during pregnancy decreases both fetal after myocardial infarction results...
Over the last several years, mesenchymal stromal cells (MSCs) have been isolated from different tissues following a variety of procedures. Here, we comparatively assess ex vivo and in properties MSCs either adipose tissue or bone marrow by purification protocols. After MSC transplantation into mouse model hindlimb ischemia, clinical histological analysis revealed that purified on adhesive substrates exerted best therapeutic activity, preserving viability promoting formation new arterioles...
Myocardial tissue engineering currently represents one of the most realistic strategies for cardiac repair. We have recently discovered ability carbon nanotube scaffolds to promote cell division and maturation in cardiomyocytes. Here, we test hypothesis that cardiomyocyte growth by altering gene expression program, implementing electrophysiological properties improving networking functional syncytia. In our study, combine microscopy, biological methodologies, calcium imaging, verify whether...
Laser therapy, recently renamed as photobiomodulation, stands a promising supportive treatment for oral mucositis induced by oncological therapies. However, its mechanisms of action and, more importantly, safety in cancer patients, are still unclear. Here we explored the anti-cancer effect 3 laser protocols, set at most commonly used wavelengths, B16F10 melanoma and carcinogenesis mouse models. While light increased cell metabolism cultured cells, vivo outcome was reduced tumor progression....
Heart failure is a disease of epidemic proportion and leading cause mortality in the world. Because cardiac myocytes are terminally differentiated cells with minimal intrinsic ability to self-regenerate, tissue engineering has emerged as one most realistic therapeutic strategies for repair. We have previously proven carbon nanotube scaffolds promote cardiomyocyte proliferation, maturation, long-term survival. Here, we tested if three-dimensional nanotube-based composites can also growth,...
Resolution of bacterial infections is often hampered by both resistance to conventional antibiotic therapy and hiding cells inside biofilms, warranting the development innovative therapeutic strategies. Here, we report efficacy blue laser light in eradicating Pseudomonas aeruginosa cells, grown planktonic state, agar plates mature vitro vivo, with minimal toxicity mammalian tissues. Results obtained using knock-out mutants point oxidative stress as a relevant mechanism which exerts its...
Therapies for patients with myocardial infarction and heart failure are urgently needed, in light of the breadth these conditions lack curative treatments. To systematically identify previously unidentified cardioactive biologicals an unbiased manner vivo, we developed cardiac FunSel, a method systematic, functional selection effective factors using library 1198 barcoded adeno-associated virus (AAV) vectors encoding mouse secretome. By pooled vector injection into heart, this was screened to...
In the last decade, carbon nanotube growth substrates have been used to investigate neurons and neuronal networks formation in vitro when guided by artificial nano-scaled cues. Besides, nanotube-based interfaces are being developed, such as prosthesis for monitoring brain activity. We recently described how alter electrophysiological synaptic responses of hippocampal culture. This observation highlighted exceptional ability this material interfering with nerve tissue growth. Here we test...
Rationale: The Notch pathway plays a key role in stimulating mammalian cardiomyocyte proliferation during development and the early postnatal life; adult zebrafish, reactivation of this is also essential to drive cardiac regeneration after injury. Objective: We wanted assess efficacy stimulation neonatal hearts as means induce myocardial infarction mice. Methods Results: In life, exit from cell cycle was paralleled by decreased signaling establishment repressive chromatin environment at...
The ability of the adult heart to regenerate cardiomyocytes (CMs) lost after injury is limited, generating interest in developing efficient cell-based transplantation therapies. Rigid carbon nanotubes (CNTs) scaffolds have been used improve CMs viability, proliferation, and maturation, but they require undesirable invasive surgeries for implantation. To overcome this limitation, we developed an injectable reverse thermal gel (RTG) functionalized with CNTs (RTG-CNT) that transitions from a...
The development of compact accelerator facilities providing high-brightness beams is one the most challenging tasks in field next-generation and cost affordable particle accelerators, to be used many fields for industrial, medical, research applications. ability shape beam longitudinal phase space, particular, plays a key role achieving high-peak brightness. Here we present new approach that allows us tune space by means plasma wakefields. electron passing through drives large wakefields are...
Plasma-based technology promises a tremendous reduction in size of accelerators used for research, medical, and industrial applications, making it possible to develop tabletop machines accessible broader scientific community. By overcoming current limits conventional pushing particles larger energies, the availability strong tunable focusing optics is mandatory also because plasma-accelerated beams usually have large angular divergences. In this regard, active-plasma lenses represent compact...
Given the clinical impact of LMNA cardiomyopathies, understanding lamin function will fulfill a need and lead to advancement in treatment heart failure. A multidisciplinary approach combining cell biology, atomic force microscopy (AFM), molecular modeling was used analyse biomechanical properties human A/C gene (LMNA) mutations (E161K, D192G, N195K) using an vitro neonatal rat ventricular myocyte model. The severity defects due three correlated with phenotype. AFM identified distinctive...
Despite the high prevalence of ischemic heart diseases worldwide, no antibody-based treatment currently exists. Starting from evidence that a specific isoform Bone Morphogenetic Protein 1 (BMP1.3) is particularly elevated in both patients and animal models myocardial infarction, here we assess whether its inhibition by monoclonal antibody reduces cardiac fibrosis. We find this collagen deposition cross-linking, paralleled enhanced cardiomyocyte survival, vivo primary cultures cells....
Abstract Atomic force microscopy (AFM) cell loading/unloading curves were used to provide comprehensive insights into biomechanical behavior of cardiomyocytes carrying the lamin A/C (LMNA) D192G mutation known cause defective nuclear wall, myopathy and severe cardiomyopathy. Our results suggested that LMNA increased maximum deformation load, stiffness fragility as compared controls. Furthermore, there seems be a connection between this adhesion since displayed loss AFM probe-to-cell membrane...
Heart failure is a morbid disorder characterized by progressive cardiomyocyte (CM) dysfunction and death. Interest in cell-based therapies growing, but sustainability of injected CMs remains challenge. To mitigate this, we developed an injectable biomimetic Reverse Thermal Gel (RTG) specifically engineered to support long-term CM survival. This RTG biopolymer provided solution-based delivery vehicle CMs, which transitioned gel-based matrix shortly after reaching body temperature. In this...
ZASP is a cytoskeletal PDZ-LIM protein predominantly expressed in striated muscle. It forms multiprotein complexes and plays pivotal role the structural integrity of sarcomeres. Mutations are associated with myofibrillar myopathy, left ventricular non-compaction dilated cardiomyopathy. The ablation its murine homologue Cypher results neonatal lethality. has several alternatively spliced isoforms, this paper we clarify nomenclature human isoforms as well their dynamics expression pattern...
Background Ankrd2 (also known as Arpp) together with Ankrd1/CARP and DARP are members of the MARP mechanosensing proteins that form a complex titin (N2A)/calpain 3 protease/myopalladin. In muscle, is located in I-band sarcomere moves to nucleus adjacent myofibers on muscle injury. myoblasts it predominantly differentiation shifts from cytoplasm. agreement its role sensor interacts both sarcomeric transcription factors. Methodology/Principal Findings Expression profiling endogenous silenced...
Previous investigations suggested that lamin A/C gene (LMNA) mutations, which cause a variety of human diseases including muscular dystrophies and cardiomyopathies, alter the nuclear mechanical properties. We hypothesized biomechanical changes may extend beyond nucleus.