A5070129349- Neonatal Respiratory Health Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Metal complexes synthesis and properties
- Respiratory Support and Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Free Radicals and Antioxidants
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Synthesis and biological activity
- Medical Imaging and Pathology Studies
- Influenza Virus Research Studies
- Congenital Diaphragmatic Hernia Studies
- Inhalation and Respiratory Drug Delivery
- Immune Response and Inflammation
- RNA modifications and cancer
- Carcinogens and Genotoxicity Assessment
- Pediatric health and respiratory diseases
- S100 Proteins and Annexins
- Air Quality and Health Impacts
- Synthesis of Organic Compounds
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Fibroblast Growth Factor Research
- Adipokines, Inflammation, and Metabolic Diseases
- Redox biology and oxidative stress
Temple University
2016-2024
Institut thématique Immunologie, inflammation, infectiologie et microbiologie
2023
National Jewish Health
2008-2019
University of Colorado Denver
2008-2019
Zero to Three
2019
Aarhus University
2014
Institut de Biologie du Développement Marseille
2014
Aix-Marseille Université
2014
University of Łódź
2004-2011
Texas A&M University System
2005-2007
Alveolar epithelium plays a pivotal role in protecting the lungs from inhaled infectious agents. Therefore, regenerative capacity of alveolar is critical for recovery these insults order to rebuild epithelial barrier and restore pulmonary functions. Here, we show that sublethal infection mice with Streptococcus pneumoniae, most common pathogen community-acquired pneumonia, led exclusive damage lung alveoli, followed by regeneration resolution inflammation. We surfactant protein C–expressing...
Alveolar Type II (ATII) cells are important targets for seasonal and pandemic influenza. To investigate the influenza-induced innate immune response in those cells, we measured global gene expression profile of highly differentiated ATII infected with influenza A virus at a multiplicity infection 0.5 4 hours 24 after inoculation. Infection stimulated significant increase mRNA concentrations many host defense-related genes, including pattern/pathogen recognition receptors, IFN, IFN-induced...
Angiogenesis, in which new blood vessels form via endothelial cell (EC) sprouting from existing vessels, is a critical event embryonic development and multiple disease processes. Many insights have been made into key EC receptors ligands/growth factors that govern angiogenesis, but intracellular molecular mechanisms of this process are not well understood. NF-E2-related factor 2 (Nrf2) transcription well-known for regulating the stress response pathologic settings, its role less appreciated....
Background Cigarette smoke (CS) is a highly complex mixture and many of its components are known carcinogens, mutagens, other toxic substances. CS induces oxidative stress cell death, this toxicity plays key role in the pathogenesis several pulmonary diseases. Methodology/Principal Findings We studied effect cigarette extract (CSE) human alveolar epithelial type I-like (ATI-like) cells. These isolated II cells that differentiating toward I phenotype vitro have lost markers express markers....
The alveolar surface is covered by an epithelium composed of 2 main cell types: type I and II cells. Alveolar (ATII) cells have a distinct morphology with apical microvilli characteristic lamellar bodies, which are the intracellular storage form pulmonary surfactant. ATII play important role in innate immunity produce secrete They proliferate to restore after damage more sensitive We developed efficient rapid method isolate purify from mice. epithelial were dissociated murine lung dispase...
BackgroundCigarette smoke is the main risk factor of pulmonary emphysema development, which characterized by alveolar wall destruction. Mitochondria are important for type II (ATII) cell metabolism due to ATP generation.MethodsWe isolated ATII cells from control non-smoker and smoker organ donors, after lung transplant patients with determine mitochondrial function, dynamics (mt) DNA damage.FindingsWe found high superoxide generation mtDNA damage in emphysema. This correlated decreased...
Electronic cigarette (e-cig) use has recently been implicated in promoting atherosclerosis. In this study, we aimed to investigate the mechanism of e-cig exposure accelerated atherosclerotic lesion development. Approach and Results: Eight-week-old ApoE-/- mice fed normal laboratory diet were exposed vapor (ECV) for 2 hours/day, 5 days/week 16 weeks. We found that ECV significantly induced lesions as examined by Oil Red O staining greatly upregulated TLR9 (toll-like receptor 9) expression...
Cigarette smoke (CS) is a main risk factor for chronic obstructive pulmonary disease (COPD). Oxidative stress induced by CS causes DNA and lung damage. Oxidant/antioxidant imbalance occurs in the distal air spaces of smokers patients with COPD. We studied effect oxidative generated both vivo vitro on murine primary alveolar type II (ATII) cells isolated from nuclear erythroid 2-related factor-2 (Nrf2)(-/-) mice. determined human ATII cell injury analyzed smoker non-smoker donors ex vivo....
Long-chain acyl-CoA dehydrogenase (LCAD) is a mitochondrial fatty acid oxidation enzyme whose expression in humans low or absent organs known to utilize acids for energy such as heart, muscle, and liver. This study demonstrates localization of LCAD human alveolar type II pneumocytes, which synthesize secrete pulmonary surfactant. The physiological role the pathway lung was subsequently studied using knock-out mice. Lung reduced LCAD−/− mice demonstrated compliance, but histological...
Rationale Alveolar type II (ATII) cells act as adult stem contributing to alveolar I (ATI) cell renewal and play a major role in idiopathic pulmonary fibrosis (IPF), supported by familial cases harbouring mutations genes specifically expressed these cells. During IPF, ATII lose their regenerative potential aberrantly express pathways epithelial–mesenchymal transition (EMT). The microRNA miR-200 family is downregulated but its effect on human IPF remains unproven. We wanted 1) evaluate the...
Abstract Aging impairs the immune responses to influenza A virus (IAV), resulting in increased mortality IAV infections older adults. However, factors within aged lung that compromise host defense remain unknown. Using a murine model and human samples, we identified prostaglandin E 2 (PGE ), as such factor. Senescent type II alveolar epithelial cells (AECs) are overproducers of PGE lung. proliferation macrophages (AMs), critical for against respiratory pathogens, via reduction oxidative...
Lipids play a central role in lung physiology and pathology; however, comprehensive lipidomic characterization of human pulmonary cells relevant to disease has not been performed. The involved host defense, including alveolar macrophages (AMs), bronchial epithelial (BECs), type II (ATIIs), were isolated from subjects analysis by LC-MS LC-MS/MS was Additionally, pieces tissue the same donors analyzed MALDI imaging MS order determine lipid localization tissue. unique distribution phospholipids...
Emphysema is caused by the cigarette smoke (CS)-induced destruction of alveolar wall septa, and CS main risk factor for chronic obstructive pulmonary disease (COPD). To study mechanisms response to this insult, we focused on oxidant-induced lung injury potential role nuclear erythroid 2-related factor-2 (Nrf2), which a key regulator antioxidant defense system. We studied protective N-acetylcysteine (NAC) against type II (ATII) cells induced in vivo vitro. ATII were isolated purified using...
Identification of biomarkers cigarette smoke -induced lung damage and early COPD is an area intense interest. Glucose regulated protein 78 kD (i.e., GRP78), a multi-functional which mediates cell responses to oxidant stress, increased in the lungs smokers serum subjects with COPD. We have suggested that secretion GRP78 by cells may explain increase To assess lung, we assayed bronchoalveolar lavage fluid (BALF) chronic non-smokers. also directly assessed acute effect material on isolated...
Infection with the novel severe acute respiratory syndrome coronavirus 2 has been associated a hypercoagulable state. Emerging data from China and Europe have consistently shown an increased incidence of venous thromboembolism (VTE). We aimed to identify VTE early predictors at our high-volume tertiary care center.We performed retrospective cohort study 147 patients who had admitted Temple University Hospital disease 2019 (COVID-19) April 1, 2020 27, 2020. first identified (pulmonary...
The early inflammatory response to influenza A virus infection contributes severe lung disease and continues pose a serious threat human health. mechanisms by which cells invade the respiratory tract remain unclear. Uncontrolled inflammation oxidative stress cause damage in infection. We have previously shown that fatty acid binding protein 5 (FABP5) has anti-inflammatory properties. speculate that, as transporter of acids, FABP5 plays an important protective role against lipids during well....
Cigarette smoke (CS) is a main source of oxidative stress and key risk factor for emphysema, which consists alveolar wall destruction. Alveolar type (AT) II cells are in the gas exchange regions lung. We isolated primary ATII from deidentified organ donors whose lungs were not suitable transplantation. analyzed cell injury obtained nonsmokers, moderate smokers, heavy smokers. DJ-1 protects induces nuclear erythroid 2-related factor-2 (Nrf2) expression, activates antioxidant defense system....
Rationale: The association between non-tuberculous mycobacterial lung disease and alpha-1-antitrypsin (AAT) deficiency is likely due, in part, to underlying emphysema or bronchiectasis. But there increasing evidence that AAT itself enhances host immunity against microbial pathogens thus could compromise protection. Objectives: goal of this project determine if augment macrophage activity mycobacteria. Methods: We compared the ability monocyte-derived macrophages cultured autologous plasma...