A5085819789- Uterine Myomas and Treatments
- Extracellular vesicles in disease
- Cancer, Lipids, and Metabolism
- MicroRNA in disease regulation
- Advanced Proteomics Techniques and Applications
- Hidradenitis Suppurativa and Treatments
- Reproductive System and Pregnancy
- Heat shock proteins research
- Prostate Cancer Treatment and Research
- RNA Research and Splicing
- TGF-β signaling in diseases
- Cellular Mechanics and Interactions
- Microscopic Colitis
- Inflammatory Biomarkers in Disease Prognosis
- Dermatology and Skin Diseases
- Hormonal and reproductive studies
- Glycosylation and Glycoproteins Research
- Vitamin D Research Studies
- Autoimmune and Inflammatory Disorders
- Galectins and Cancer Biology
- Caveolin-1 and cellular processes
- Sexual function and dysfunction studies
- Endometriosis Research and Treatment
- S100 Proteins and Annexins
- Cardiomyopathy and Myosin Studies
IRCCS Materno Infantile Burlo Garofolo
2016-2025
University of Trieste
2015
University of Ferrara
2011
To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum pregnant women, who later developed severe preeclampsia (sPE) third trimester pregnancy (n = 24) compared to women with normal 24).Sera from 12-14-week-gestation whole blood were subjected microarray analysis TaqMan Low Density Array chips (human microRNA panel V3.0), and quantitative real-time polymerase chain reaction.By using chip technology, 19 mature miRNAs appeared group sPE...
Background/Objectives: Endometrial cancer (EC) is the second most frequent gynecological malignant tumor in postmenopausal women. Pathogenic mechanisms related to onset and development of disease are still unknown. To identify dysregulated proteins associated with EC we exploited a combined vitro/in silico approach analyzing proteome exosomes advanced MS techniques annotating their results by using Chymeris1 AI tools. Methods: this aim pilot study, performed deep proteomics analysis high...
The BCS1L gene encodes a mitochondrial chaperone which inserts the Fe2S2 iron–sulfur Rieske protein into nascent electron transfer complex III. Variants in are associated with spectrum of disorders, ranging from mild to severe phenotypes. Björnstad syndrome, milder condition, is characterized by sensorineural hearing loss (SNHL) and pili torti. More disorders include Complex III Deficiency, leads neuromuscular metabolic dysfunctions multi-systemic issues Growth Retardation, Aminoaciduria,...
Endometrial cancer is the most common gynecologic malignancy arising from endometrium. Identification of serum biomarkers could be beneficial for its early diagnosis. We have used 2D-Difference In Gel Electrophoresis (2D-DIGE) coupled with Mass Spectrometry (MS) procedures to investigate proteome 15 patients endometrial and non-cancer subjects. identified 16 proteins diagnostic potential, considering only spots a fold change in %V ≥ 1.5 or ≤0.6 intensity, which were statistically significant...
Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease with multifactorial aetiology that involves strict interplay between genetic factors, immune dysregulation and lifestyle. Familial forms represent around 40% of total HS cases show an autosomal dominant mode inheritance the disease. In this study, we conducted whole-exome sequence analysis on Italian family 4 members encompassing vertical transmission HS. Focusing rare damaging variants, identified insertion one nucleotide...
Endometrial cancers (ECs) are mostly adenocarcinomas arising from the inner part of uterus. The identification serum biomarkers, either soluble or carried in exosome, may be useful making an early diagnosis. We used label-free quantification mass spectrometry (LFQ-MS)-based proteomics to investigate proteome exosomes albumin-depleted 12 patients with EC, as compared healthy controls. After and statistical analysis, we found significant changes abundance (p < 0.05) 33 proteins EC vs. control...
Endometrial cancer (EC) is the most frequent gynaecologic in postmenopausal women. We used 2D-DIGE and mass spectrometry to identify candidate biomarkers endometrial cancer, analysing serum protein contents of 10 patients versus control subjects. Using gel-based proteomics, we identified 24 biomarkers, considering only spots with a fold change volume percentage ≥ 1.5 or intensity ≤ 0.6, which were significantly different between cases controls (p < 0.05). Western blotting analysis both...
Epithelial-to-Mesenchymal Transition (EMT) is relevant in malignant growth and frequently correlates with worsening disease progression due to its implications metastases resistance therapeutic interventions. Although EMT known occur several types of solid tumors, the information concerning tumors arising from epithelia bile tract still limited. In order approach problem cholangiocarcinoma, we decided investigate changes protein expression occurring two cell lines under conditions leading as...
Endometrial cancer arises from the endometrium. It has a slow progression and reported survival rate of 75%. The identification soluble biomarkers in uterine aspirate may be very useful for its early diagnosis. Uterine aspirates 10 patients with endometrial 6 non-endometrial controls were analyzed by two-dimensional gel electrophoresis coupled mass spectrometry western blotting data verification. A total 25 proteins fold change %V ≥2 or ≤0.5 intensity observed to significantly (P<0.05). From...
The exact role of malignant ascites in the development intraperitoneal metastases remains unclear, and mechanisms by which extracellular vesicles (EVs) promote tumor progression pre-metastatic niche have not been fully discovered. In this study, we characterized from high-grade epithelial ovarian cancer patients. Small-EVs (30-150 nm) were isolated two sources-the bulk ascitic fluid-derived cell cultures-and assessed with a combination imaging, proteomic profiling, protein expression...
Uterine leiomyomas are benign smooth muscle cell tumors originating from the myometrium. In this study we focus on leiomyoma and normal myometrium phosphoproteome, to identify differentially phosphorylated proteins involved in tumorigenic signaling pathways, anti-apoptotic processes survival. We obtained paired tissue samples of seven adjacent myometria analyzed phosphoproteome by two-dimensional gel electrophoresis (2-DE) combined with immobilized metal affinity chromatography (IMAC) Pro-Q...
Endometrial cancer (EC) is the most common gynecologic malignancy, and it arises in inner part of uterus. Identification serum biomarkers essential for diagnosing disease at an early stage. In this study, we selected 44 healthy controls type I EC tumor stage 1, used Immuno-oncology panel Target 96 Oncology III to simultaneously detect levels 92 cancer-related proteins serum, using a proximity extension assay. By applying methodology, identified 20 proteins, associated with outcome binary...
Endometrial cancer (EC) is the most common gynecologic malignancy of endometrium. This study focuses on EC and normal endometrium phosphoproteome to identify differentially phosphorylated proteins involved in tumorigenic signalling pathways which induce growth. We obtained tissue samples from 8 types I at tumour stage 1 endometria. analyzed by two-dimensional differential gel electrophoresis (2D-DIGE), combined with immobilized metal affinity chromatography (IMAC) mass spectrometry for...
Uterine leiomyoma is the most common benign smooth muscle cell tumor of uterus. Proteomics a powerful tool for analysis complex mixtures proteins. In our study, we focused on proteins that were upregulated in compared to myometrium. Paired samples eight leiomyomas and adjacent myometrium obtained submitted two-dimensional gel electrophoresis (2-DE) mass spectrometry protein identification Western blotting 2-DE data validation. The comparison between patterns revealed 24 significantly (p <...
Endometrial cancer (EC) is the most frequent gynecologic in postmenopausal women. Pathogenetic mechanisms that are related to onset and progression of disease largely still unknown. A multi-omics strategy can help identify altered pathways could be targeted for improving therapeutical approaches. In this study we used a approach on four EC cell lines identification common dysregulated type 1 2 ECs. We analyzed proteomics metabolomics AN3CA, HEC1A, KLE ISHIKAWA by mass spectrometry. The...
Among gynecological cancers, endometrial cancer is the most common in developed countries. Extracellular vesicles (EVs) are cell-derived membrane-surrounded that contain proteins involved immune response and apoptosis. A deep proteomic approach can help to identify dysregulated extracellular matrix (ECM) EVs correlated key pathways for tumor development. In this study, we used a proteomics correlating two acquisitions—data-dependent acquisition (DDA) data-independent (DIA)—on from...
Aquaporin-3 (AQP3) is an aquaglyceroporin and peroxiporin that plays a crucial role in skin barrier homeostasis. Dysregulated AQP3 expression has been observed different inflammatory conditions. Hidradenitis Suppurativa (HS) autoinflammatory keratinization disease typically appears between 10 21 years of age, characterized by alteration
Uterine leiomyoma is the most common female reproductive tract benign tumor. Little known about protein composition and changes in interstitial fluid (IF). The present study focused on abundance IF of leiomyoma. Leiomyoma IFs adjacent myometrial were obtained analyzed by two-dimensional electrophoresis (2-DE) coupled with mass spectrometry western blotting for 2-DE data validation. A total 25 unique proteins observed to change significantly (P<0.05). Of these different abundance, 22 had not...
The aim of the present study was to analyze protein composition ductal breast carcinoma and surrounding normal tissue in individual patients using comparative 2D proteomics mass spectrometry detect candidate disease biomarkers for diagnosis prognosis. Samples cancerous obtained form 28 were analyzed. Chaperonins cytoskeletal proteins predominated among 11 which major changes abundance detected. Of these with an altered expression, 2 had a decreased expression 9 increased expression. In...