To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum pregnant women, who later developed severe preeclampsia (sPE) third trimester pregnancy (n = 24) compared to women with normal 24).Sera from 12-14-week-gestation whole blood were subjected microarray analysis TaqMan Low Density Array chips (human microRNA panel V3.0), and quantitative real-time polymerase chain reaction.By using chip technology, 19 mature miRNAs appeared group sPE...

Biospecific interaction analysis (BIA) was performed using surface plasmon resonance (SPR) and biosensor technologies to detect genetically modified Roundup Ready soybean gene sequences. We first immobilized, on SA sensor chips, single-stranded biotinylated oligonucleotides containing lectin sequences, the efficiency of hybridization oligonucleotide probes differing in length determined. Second, we immobilized PCR products from nontransgenic soybeans (genomes carrying only gene), as well...

New drugs would be beneficial to fight resistant HIV strains, in particular those capable of interfering with essential viral functions other than targeted by highly active antiretroviral therapy drugs. Despite the central role played Tat protein transcription, a search for vegetable extracts able hamper this important function was never carried out. In work, we evaluated chemical composition and possible interference oil from Thymus vulgaris , Cananga odorata Cymbopogon citratus Rosmarinus...

Screening a cDNA library from human skeletal muscle and cardiac with probe derived junctin led to the isolation of two groups clones. The first group displayed deduced amino acid sequence that is 84% identical dog heart junctin, whereas second had single open reading frame encoded polypeptide predicted mass 33 kDa, whose 78 NH₂-terminal residues are its COOH terminus domain aspartyl β-hydroxylase, member α-ketoglutarate-dependent dioxygenase family. We named latter junctate....

The human leukaemic K562 cell line can be induced in vitro to undergo erythroid differentiation by a variety of chemical compounds, including haemin, butyric acid, 5‐azacytidine and cytosine arabinoside. Differentiation cells is associated with an increased expression embryo‐fetal globin genes, such as the ζ, ε γ genes. Therefore has been proposed useful model system determine therapeutic potential new differentiating compounds well study molecular mechanism(s) regulating changes embryonic...

Among the phenolic acids tested on K562 cell line, a model of chronic myeloid leukemia (CML), caffeic acid (CA) was biologically active sensitive and imatinib (IM)-resistant cells at micro-molar concentration, either in terms reduction proliferation or triggering apoptosis. The CA treatment provoked mitochondrial membrane depolarization, genomic DNA fragmentation phosphatidylserine exposure, hallmarks Cell cycle analysis following with comparable cytotoxic concentrations IM showed marked...

Glucose transporter-4 (GLUT4) represents the major glucose transporter isoform responsible for uptake into insulin-sensitive cells, primarily in skeletal muscle and adipose tissues. In insulin-resistant conditions, such as type 2 diabetes mellitus, GLUT4 expression and/or translocation to cell plasma membrane is reduced, compromising energy metabolism. Therefore, use of synthetic or naturally occurring molecules able stimulate a good tool alternative treatments insulin resistance. The...

We determined whether peptide nucleic acids (PNAs) are able to interact with NF-kappaB p52 transcription factor. The binding of DNA-DNA, DNA-PNA, PNA-DNA, and PNA-PNA hybrid molecules carrying the sites human immunodeficiency type 1 long terminal repeat was studied by (i) biospecific interaction analysis (BIA) using surface plasmon resonance technology, (ii) electrophoretic mobility shift, (iii) DNase I footprinting, (iv) UV cross-linking assays. Our results demonstrate that does not...

Abstract Peptide nucleic acids (PNAs) containing an insert of three chiral monomers based on D ‐lysine (‘chiral box’) were synthesized and used as probes in Biospecific Interaction Analysis (BIA) for the recognition DNA W1282X point mutation cystic fibrosis gene. Hybridization experiments carried out solution showed enhanced mismatch when compared with analogous achiral PNAs oligonucleotides. The signal intensity was lower, but selectivity Biacore response found to be much higher than that...

In this paper we demonstrate that peptide nucleic acids (PNAs) are excellent probes able to detect the W1282X point mutation of cystic fibrosis (CF) gene when biospecific interaction analysis (BIA) by surface plasmon resonance (SPR) and biosensor technologies is performed. The results reported here suggest BIA an easy, fast, automatable approach for detecting mutations CF, allowing real-time monitoring hybridization between 9-mer CF PNA target biotinylated PCR products generated from...

DNA-binding drugs have been reported to be able interfere with the activity of transcription factors in a sequence-dependent manner, leading alteration transcription. This and similar effects could important practical applications experimental therapy many human pathologies, including neoplastic diseases viral infections. The analysis biological by footprinting, gel retardation, polymerase chain reaction, vitro studies does not allow real time study binding DNA dissociation generated...

The 'Lab-on-a-chip technology' involves miniaturization of complex analytical procedures and is expected to enable laboratory testing move from the central employing equipment into non-laboratory settings. We report application a printed circuit board (PCB)-based chip, generating dielectrophoretic (DEP)-based cylinder-shaped cages for separation recovery white blood cells erythrocytes. This possibility interest develop low-cost Lab-on-a-chip devices diagnostic purposes. Accordingly, we...

The isolation of rare cells, such as fetal nucleated red blood cells and trophoblasts, from maternal for non-invasive prenatal diagnosis is a new field research exhibiting several difficulties since this strategy requires unresolved basic technological protocols successful outcome. However, achievements in the Laboratory-on-a-chip (Lab-on-a-chip) technology have provided clear advancements projects aimed at biological fluids. Among most interesting approaches are those based on...

In several types of thalassemia (including beta(0)39-thalassemia), stop codon mutations lead to premature translation termination and mRNA destabilization through nonsense-mediated decay. Drugs (for instance aminoglycosides) can be designed suppress termination, inducing a ribosomal readthrough. These findings have introduced new hopes for the development pharmacologic approach cure this disease. However, effects aminoglycosides on globin carrying beta-thalassemia not yet been investigated....

Despite curcumin (CUR) inhibiting cell proliferation in vitro by activating apoptotic death, its use pharmacological therapy is hampered poor solubility, low stability biological fluids, and rapid removal from the body. Therefore, CUR-derivatives with better chemical–physical characteristics are needed. The bis-ketone moiety of CUR strongly influences slightly alkaline solutions such as plasma. Here, we considered replacement isoxazole, beta-enamine, or oxime groups to obtain more stable...

Surface plasmon resonance (SPR) based biosensors have been described for the identification of genetically modified organisms (GMO) by biospecific interaction analysis (BIA). This paper describes design and testing an SPR-based BIA protocol quantitative determinations GMOs. Biotinylated multiplex Polymerase Chain Reaction (PCR) products from nontransgenic maize as well powders containing 0.5 2% Bt-176 sequences were immobilized on different flow cells a sensor chip. After immobilization,...

Isatinyl and indanyl nitrones (INs) were synthesized evaluated for their antiproliferative activity antioxidant properties.

In the present paper, we applied surface plasmon resonance (SPR) and biosensor technologies for biospecific interaction analysis (BIA) to detect ΔF508 mutation (F508del) of cystic fibrosis transmembrane regulator (CFTR) gene in both homozygous as well heterozygous human subjects. The proposed method is divided into three major steps. first step immobilization on a SA5 sensor chip two biotinylated oligonucleotide probes (one normal, N-508, other mutant, ΔF508) that are able hybridize CFTR...

In the present paper, biospecific interaction analysis (BIA) was performed using surface plasmon resonance (SPR) and biosensor technologies to detect Trp1282Ter mutation (W1282X) of cystic fibrosis (CF) transmembrane conductance regulator (CFTR) gene. We first immobilized on a SA5 sensor chip single-stranded biotinylated oligonucleotide containing sequence involved in this mutation, efficiency hybridization probes differing length determined. Second, we different chips polymerase-chain...

We have investigated the effects of aromatic polyamidines on HIV-1 transcription. found a block to Tat-induced transcription assessed by inhibition CAT activity in HL3T1 cells at concentration lower than IC50 value, suggesting that molecules with three (TAPB) and four (TAPP) benzamidine rings could be useful against HIV-1. In contrast, only two (DAPP) did not reasoned this effect due binding TAPB TAPP TAR RNA. By EMSA filter assays, we studied possible interactions Wild-type RNA or mutations...