A5030568464- Hemoglobinopathies and Related Disorders
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Iron Metabolism and Disorders
- Prenatal Screening and Diagnostics
- DNA and Nucleic Acid Chemistry
- Erythrocyte Function and Pathophysiology
- Cystic Fibrosis Research Advances
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Blood groups and transfusion
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- SARS-CoV-2 and COVID-19 Research
- Neonatal Respiratory Health Research
- Circular RNAs in diseases
- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- Genomics, phytochemicals, and oxidative stress
- Synthesis and biological activity
- Garlic and Onion Studies
- RNA Research and Splicing
- Genomics and Rare Diseases
University of Ferrara
2016-2025
Consorzio Interuniversitario per le Biotecnologie
2021-2025
Lega Italiana per la Lotta ai Tumori
2014
Pontifícia Universidade Católica de Goiás
2009
Fundação de Apoio a Pesquisa do Estado de Goiás
2009
Abstract As an inherited disorder characterized by severe pulmonary disease, cystic fibrosis could be considered a comorbidity for coronavirus disease 2019. Instead, current clinical evidence seems to heading in the opposite direction. To clarify whether host factors expressed Cystic Fibrosis epithelia may influence 2019 progression, here we describe expression of SARS-CoV-2 receptors primary airway epithelial cells. We show that angiotensin converting enzyme 2 (ACE2) and localization are...
IL-8 released from bronchial epithelial cells infected with Pseudomonas aeruginosa plays a crucial role in the chronic lung pathology of patients affected by cystic fibrosis. Novel anti-inflammatory approaches will benefit thorough understanding regulatory mechanisms involved transcription this chemokine to identify potential pharmacological targets. We addressed issue investigating phosphoproteins and factors (TFs) on gene human IB3-1, CuFi-1, Calu-3 cells. P. increased basal...
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Abstract The application of Peptide Nucleic Acids (PNAs), mimics DNA lacking the sugar-phosphate backbone, for antisense/anti-gene therapy and gene editing is limited by their low uptake cells. Currently, no simple efficient delivery systems methods are available to solve this open issue. One most promising approach modification PNA structure through covalent linkage poliarginine tails, but means that every intended be internalized must modified. Herein we report results relative ability a...
Abstract: We studied the effects of rapamycin on cultures erythroid progenitors derived from peripheral blood 10 β ‐thalassaemia patients differing widely with respect to their potential produce foetal haemoglobin (HbF). For this, we employed two‐phase liquid culture procedure for growing progenitors, high performance chromatography analysis HbF production and reverse transcription polymerase chain reaction quantification accumulation globin mRNAs. The results demonstrated that induced an...
Abstract Herein we describe the activity of a peptide nucleic acid (PNA) that targets microRNA‐210 (miR‐210), which is associated with hypoxia and modulated during erythroid differentiation. PNAs directed against miR‐210 were designed to bind high affinity target RNA strand undergo efficient uptake in cells. A polyarginine–PNA conjugate (Rpep‐PNA‐a210) showed both very for into cells without need transfection reagents. An unmodified PNA same sequence displayed ability RNA, but cellular was...
The activity of a peptide nucleic acid (PNA) targeting cancer-associated microRNA-221 is described. PNAs against miR-221 were designed in order to bind very efficiently the target RNA strand and undergo efficient uptake cells. A polyarginine-PNA conjugate targeted (Rpep-PNA-a221) showed both high affinity for cellular without addition transfection reagents. Unmodified PNA with same sequence displayed binding, but was poor. Consistently, only Rpep-PNA-a221 strongly inhibited miR-221....
The biological activity of a combined treatment U251, U373 and T98G glioma cell lines with two anti-miR PNAs, directed against miR‑221 miR‑222 conjugated an ocataarginine tail (R8-PNA-a221 R8-PNA-a222) for efficient cellular delivery, was determined. Apoptosis analyzed, the effect cells either or both PNAs on reversion drug-resistance phenotype assessed in temozolomide-resistant line. Selectivity PNA/miRNA interactions studied by surface plasmon resonance (SPR)-based Biacore analysis....
Peptide nucleic acids (PNAs) are very useful tools for gene regulation at different levels, but in particular the last years their use targeting microRNA (anti-miR PNAs) has provided impressive advancements. In this respect, microRNAs related to repression of cystic fibrosis transmembrane conductance regulator (CFTR) gene, which is defective fibrosis, great importance development new type treatments. paper we propose an anti-miR PNA miR-145, a reported suppress CFTR expression....
Gene editing by the CRISPR-Cas9 nuclease system technology can be considered among most promising strategies to correct hereditary mutations in a variety of monogenic diseases. In this paper, we present for first time correction, gene editing, β039-thalassemia mutation, one frequent Mediterranean area. The results obtained demonstrated presence normal β-globin genes after correction mutation performed on erythroid precursor cells from homozygous patients. This was allele-specific PCR and...
Introduction: β-thalassemia is caused by autosomal mutations in the β-globin gene, which induce absence or low-level synthesis of erythroid cells. It widely accepted that a high production fetal hemoglobin (HbF) beneficial for patients with β-thalassemia. Sirolimus, also known as rapamycin, lipophilic macrolide isolated from strain Streptomyces hygroscopicus serves strong HbF inducer vitro and vivo. In this study, we report biochemical, molecular, clinical results sirolimus-based NCT03877809...
Congenital myopathies are genetically and clinically heterogeneous conditions causing severe muscle weakness, mutations in the ryanodine receptor gene (RYR1) represent most frequent cause of these conditions. A common feature diseases caused by recessive RYR1 is a decrease 1 protein content muscle. The aim present investigation was to gain mechanistic insight into causes this reduced 1. We found that biopsies patients with exhibit decreased expression muscle-specific microRNAs, increased DNA...
The involvement of microRNAs in the control repressors human γ-globin gene transcription has been firmly demonstrated, as described for miR-486-3p mediated down-regulation BCL11A. On other hand, we have reported that miR-210 is involved erythroid differentiation and, possibly, up-regulation. In present study, identified coding sequence BCL11A a possible target miR-210. following results sustain this hypothesis: (a) interactions between and site were demonstrated by SPR-based biomolecular...