A5027802626- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Sphingolipid Metabolism and Signaling
- Virus-based gene therapy research
- Immune Response and Inflammation
- Tracheal and airway disorders
- Respiratory viral infections research
- Extracellular vesicles in disease
- Antimicrobial Peptides and Activities
- Asthma and respiratory diseases
- Infant Nutrition and Health
- NF-κB Signaling Pathways
- Carbohydrate Chemistry and Synthesis
- Circular RNAs in diseases
- Bacterial biofilms and quorum sensing
- Pneumocystis jirovecii pneumonia detection and treatment
- Pediatric health and respiratory diseases
- Ion channel regulation and function
- Andrographolide Research and Applications
- Helicobacter pylori-related gastroenterology studies
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
University of Verona
2014-2024
Azienda Ospedaliera Universitaria Integrata Verona
2006-2023
Istituto Oncologico Veneto
2017
Laboratory of Molecular Genetics
2008
Cystic Fibrosis Research Foundation
1991-2003
University of Brescia
1989
Abstract As an inherited disorder characterized by severe pulmonary disease, cystic fibrosis could be considered a comorbidity for coronavirus disease 2019. Instead, current clinical evidence seems to heading in the opposite direction. To clarify whether host factors expressed Cystic Fibrosis epithelia may influence 2019 progression, here we describe expression of SARS-CoV-2 receptors primary airway epithelial cells. We show that angiotensin converting enzyme 2 (ACE2) and localization are...
IL-8 released from bronchial epithelial cells infected with Pseudomonas aeruginosa plays a crucial role in the chronic lung pathology of patients affected by cystic fibrosis. Novel anti-inflammatory approaches will benefit thorough understanding regulatory mechanisms involved transcription this chemokine to identify potential pharmacological targets. We addressed issue investigating phosphoproteins and factors (TFs) on gene human IB3-1, CuFi-1, Calu-3 cells. P. increased basal...
ABSTRACT The innate immune response to adenovirus (Ad)-derived gene transfer vectors has been shown initiate immediately after interaction of Ad with respiratory epithelial cells, through the induction extracellular signal-regulated kinase 1 and 2 (ERK1/2) JNK mitogen-activated protein (MAPK), nuclear factor κB (NF-κB), different proinflammatory genes. serotypes or 5 (Ad2/5) enter epithelia initial binding fiber coxsackievirus-adenovirus receptor (CAR) or, alternatively, cell surface heparan...
Peptide nucleic acids (PNAs) are very useful tools for gene regulation at different levels, but in particular the last years their use targeting microRNA (anti-miR PNAs) has provided impressive advancements. In this respect, microRNAs related to repression of cystic fibrosis transmembrane conductance regulator (CFTR) gene, which is defective fibrosis, great importance development new type treatments. paper we propose an anti-miR PNA miR-145, a reported suppress CFTR expression....
Newborn screening (NBS) for cystic fibrosis (CF) has been gradually established in several countries, but scant data are available on its long-term effects survival. Our objective was to evaluate the of CF NBS 586 patients, diagnosed and followed between 1971 2014 at Verona Centre were analysed. Eligibility confirmed 342 cases by NBS, 101 with meconium ileus 143 through symptoms (44 out false negatives). The primary end-point 30-year overall survival patients NBS. Patients grouped according...
Pseudomonas aeruginosa colonization, prominent inflammation with massive expression of the neutrophil chemokine IL-8, and luminal infiltrates neutrophils are hallmarks chronic lung disease in patients cystic fibrosis (CF). The nociceptive transient receptor potential ankyrin (TRPA) 1 calcium channels have been recently found to be involved nonneurogenic inflammation. Here, we investigate role TRPA1 CF respiratory inflammatory models vitro. Expression was evaluated tissue sections cells by...
The psoralen-related compound, 4,6,4'-trimethylangelicin (TMA) potentiates the cAMP/PKA-dependent activation of WT-CFTR and rescues F508del-CFTR-dependent chloride secretion in both primary secondary airway cells homozygous for F508del mutation. We recently demonstrated that TMA, like Lumacaftor (VX-809), stabilizes first membrane-spanning domain (MSD1) enhances interface between NBD1 ICL4 (MSD2). TMA also anti-inflammatory properties, via reduction IL-8 expression, thus making a promising...
Chronic inflammatory response in the airway tract of patients affected by cystic fibrosis is characterized an excessive recruitment neutrophils to bronchial lumina, driven chemokine interleukin (IL)-8. We previously found that 5-methoxypsoralen reduces Pseudomonas aeruginosa-dependent IL-8 transcription epithelial cell lines, with IC(50) 10 μM (Nicolis E, Lampronti I, Dechecchi MC, Borgatti M, Tamanini A, Bezzerri V, Bianchi N, Mazzon Mancini Giri MG, Rizzotti P, Gambari R, Cabrini G. Int...
Abstract Respiratory insufficiency is the major cause of morbidity and mortality in patients affected by cystic fibrosis (CF). An excessive neutrophilic inflammation, mainly orchestrated release IL-8 from bronchial epithelial cells amplified chronic bacterial infection with Pseudomonas aeruginosa, leads to progressive tissue destruction. The anti-inflammatory drugs presently used CF have several limitations, indicating need for identifying novel molecular targets. To address this issue, we...
Extracts from Nigella arvensis L. seeds, which are widely used as anti-inflammatory remedies in traditional medicine of Northern Africa, were able to inhibit the expression pro-inflammatory neutrophil chemokine Interleukin (IL)-8 Cystic Fibrosis (CF) bronchial epithelial IB3-1 cells exposed Gram-negative bacterium Pseudomonas aeruginosa. The chemical composition extracts led identification three major components, β-sitosterol, stigmasterol and campesterol, most abundant phytosterols,...
but so far the functional effect of PKC-dependent phosphorylation has not been clarified.We investigated PKC on CFTR-mediated C1transport by treating with phorbol 12-myristate 13-acetate (PMA), cell line C127i stably expressing CFTR wild type (C127 CFTRw/t), or bearing most common mutation deltaF508 CFTRdF508).We show that PMA activates C1-efflux in C127 CFTRwIt, CFTRdF508 and C127i.The PMA-dependent activation is mediated increase intracellular [CAMP] result a primary K+ conductive...