A5049473177- Blood properties and coagulation
- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Toxin Mechanisms and Immunotoxins
- Erythrocyte Function and Pathophysiology
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Cannabis and Cannabinoid Research
- Skin and Cellular Biology Research
- Plant Surface Properties and Treatments
- Mast cells and histamine
- Phytochemistry and biological activity of medicinal plants
- Platelet Disorders and Treatments
- Food Allergy and Anaphylaxis Research
- Hemoglobinopathies and Related Disorders
- Effects and risks of endocrine disrupting chemicals
- Biopolymer Synthesis and Applications
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Plant Stress Responses and Tolerance
- Pharmacological Effects and Assays
- Curcumin's Biomedical Applications
- Phytochemicals and Antioxidant Activities
- Polysaccharides Composition and Applications
- Melamine detection and toxicity
University of Rome Tor Vergata
2015-2024
Istituto Dermopatico dell'Immacolata
2000
University of the Basque Country
2000
Weatherford College
1998-2000
Ohio University
1998
University of Naples Federico II
1997
Eunice Kennedy Shriver National Institute of Child Health and Human Development
1990-1995
University of Ferrara
1995
University of Padua
1995
National Institutes of Health
1985-1994
Incubation of chloroplasts Helianthus tuberosus with labelled putrescine and/or spermidine and proteolytic digestion their trichloroacetate-soluble -insoluble proteins revealed the presence N-(gamma-glutamyl)-putrescine, N1,N4-bis-(gamma-glutamyl)-putrescine N1,N8-bis-(gamma-glutamyl)spermidine. This finding may be regarded as unequivocal proof transglutaminase activity in chloroplasts. In addition, recovery or acetylspermidine from incubated [3H]putrescine [3H]spermidine respectively...
We have investigated polyamine metabolism in primary cultures of mouse epidermal cells.These cells, which grow at low Caa* levels as a monolayer with characteristics basal terminally differentiate when the extracellular Ca2+ level is raised above 1 mM.The cellular free polyamines were measured, and, after incubation cell ['Hlputrescine, distribution label both acid-soluble and acid-insoluble components was examined.Free reduced cells induced to differentiate.Treatment retinoic acid, prevents...
Glucocorticoids (GC) are used widely for the treatment of patients with various disorders, including autoimmune diseases, allergies, and lymphoproliferative disorders. Glucocorticoid therapy is often limited by several adverse reactions associated GC excess. Excess can elicit a variety symptoms signs, growth retardation in children; immunosuppression; cardiovascular disorders like hypertension atherosclerosis; osteoporosis; myopathy; diabetes mellitus. Currently, attention focused on...
New drugs would be beneficial to fight resistant HIV strains, in particular those capable of interfering with essential viral functions other than targeted by highly active antiretroviral therapy drugs. Despite the central role played Tat protein transcription, a search for vegetable extracts able hamper this important function was never carried out. In work, we evaluated chemical composition and possible interference oil from Thymus vulgaris , Cananga odorata Cymbopogon citratus Rosmarinus...
Osteopontin (OP) is a component of extracellular, bone, and urinary stone matrices, but the mechanism by which it stably incorporated into such matrices remains unknown. By SDS-PAGE analysis [125I]OP, treated with catalytic amount TG, we first demonstrate both intra- intermolecular covalent cross-linking OP. Most importantly, products generated from reactions containing OP, Fn, TG SDS-PAGE, autoradiography, Western blotting using either OP or Fn antibody, quantitation TG-catalyzed...
Poly(vinyl alcohol) microcapsules have been tailored as carriers to deliver camptothecin, an anticancer drug poorly soluble in water. The capsules reacted with a chitosan--folate complex order selectively target cancer cells overexpressing the folic acid receptor. Microcapsules decorated characterized their uptake and release of following absorption band at λ = 370 nm diagnostic molecule. selectivity chitosan-folate targeting has demonstrated by fluorescence microscopy using HeLa cells,...
Among the phenolic acids tested on K562 cell line, a model of chronic myeloid leukemia (CML), caffeic acid (CA) was biologically active sensitive and imatinib (IM)-resistant cells at micro-molar concentration, either in terms reduction proliferation or triggering apoptosis. The CA treatment provoked mitochondrial membrane depolarization, genomic DNA fragmentation phosphatidylserine exposure, hallmarks Cell cycle analysis following with comparable cytotoxic concentrations IM showed marked...
We have previously demonstrated that when phospholipase A2 is treated with either tissue tranaglutaminase or human plasma Factor XHIa, a striking increase of its catalytic activity observed, due to the formation an intramolecular e-(7-glutamyl)-lysine crosslink [Cordella-Miele et al. (1990) J. Biol. Chem. 265,17180–17188]. Here, we report effect transglutaminase substrates such as mono-, di-, and polyamines on this transglutaminasecatalyzed post-translational modification A2. Incorporation...
Soil salinity is considered one of the most severe abiotic stresses in plants; plant acclimation to could be a tool improve salt tolerance even sensitive genotype. In this work we investigated physiological mechanisms underneath response gradual and prolonged exposure sodium chloride cultivars Brassica napus L. Fifteen days old seedlings Dynastie (salt tolerant) SY Saveo sensitive) were progressively exposed increasing soil conditions for 60 days. Salt plants both showed reductions biomass,...
Glucose transporter-4 (GLUT4) represents the major glucose transporter isoform responsible for uptake into insulin-sensitive cells, primarily in skeletal muscle and adipose tissues. In insulin-resistant conditions, such as type 2 diabetes mellitus, GLUT4 expression and/or translocation to cell plasma membrane is reduced, compromising energy metabolism. Therefore, use of synthetic or naturally occurring molecules able stimulate a good tool alternative treatments insulin resistance. The...