A5030764152- Wound Healing and Treatments
- Cardiac Fibrosis and Remodeling
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Diabetic Foot Ulcer Assessment and Management
- Reproductive Biology and Fertility
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Viral Infections and Immunology Research
- Ubiquitin and proteasome pathways
- Hippo pathway signaling and YAP/TAZ
- Body Contouring and Surgery
- Virus-based gene therapy research
- Cardiomyopathy and Myosin Studies
- Dental Research and COVID-19
- Medical and Biological Ozone Research
- Pulmonary Hypertension Research and Treatments
- RNA modifications and cancer
- Neonatal Respiratory Health Research
- Endometriosis Research and Treatment
- Diagnosis and Treatment of Venous Diseases
- Elasticity and Material Modeling
- Uterine Myomas and Treatments
- Ovarian function and disorders
- Congenital heart defects research
University of Trieste
2020-2025
International Centre for Genetic Engineering and Biotechnology
2019-2025
International Centre for Genetic Engineering and Biotechnology
2022
Resolution of bacterial infections is often hampered by both resistance to conventional antibiotic therapy and hiding cells inside biofilms, warranting the development innovative therapeutic strategies. Here, we report efficacy blue laser light in eradicating Pseudomonas aeruginosa cells, grown planktonic state, agar plates mature vitro vivo, with minimal toxicity mammalian tissues. Results obtained using knock-out mutants point oxidative stress as a relevant mechanism which exerts its...
Pulmonary fibrosis is a devastating disease, in which fibrotic tissue progressively replaces lung alveolar structure, resulting chronic respiratory failure. Alveolar type II cells act as epithelial stem cells, being able to transdifferentiate into I mediate gas exchange, thus contributing homeostasis and repair after damage. Impaired transdifferentiation emerging major pathogenetic mechanism driving both onset progression of the lung. Here, we show that endothelial secrete angiocrine factors...
The loss of skin integrity has always represented a major challenge for clinicians dealing with dermal defects, such as ulcers (diabetic, vascular and chronic), postoncologic resections (i.e., radical vulvectomy) or dermatologic disorders. introduction in recent decades acellular matrices (ADMs) supporting the repair restoration functionality significant step toward achieving clean wound before performing grafts. Hard-to-heal generally depend on local ischemia nonadequate vascularization. In...
Abstract Background New drugs to tackle the next pathway or mutation fueling cancer are constantly proposed, but 97% of them doomed fail in clinical trials, largely because they identified by cellular silico screens that cannot predict their vivo effect. Methods We screened an Adeno-Associated Vector secretome library (> 1000 clones) directly a mouse model and validated therapeutic effect first hit, EMID2, both orthotopic genetic models lung pancreatic cancer. Results EMID2 overexpression...
Abstract Nonhealing wounds place a significant burden on both quality of life affected patients and health systems. Skin substitutes are applied to promote the closure nonhealing wounds, although their efficacy is limited by inadequate vascularization. The stromal vascular fraction (SVF) from adipose tissue promising therapy overcome this limitation. Despite few successful clinical trials, its incorporation in routine has been hampered inconsistent results. All these studies concluded...
The current COVID-19 pandemic has highlighted the importance of aerosol-based transmission human pathogens; this therefore calls for novel medical devices which are able to sterilize contaminated aerosols. Here we describe a new laser device droplets containing either viruses or bacteria. Using engineered viral particles, determined 10,600 nm wavelength as most efficient and exploitable source be manufactured in commercial device. Given lack existing working models reproduce aerosol living...
A major obstacle in inducing therapeutic angiogenesis the heart is inefficient gene transfer to endothelial cells (ECs). Here, we identify compounds able enhance permissiveness of cardiac ECs adeno-associated virus (AAV) vectors, which stand as ideal tools for vivo delivery. We screened a library >1,500 US Food and Drug Administration (FDA)-approved drugs, combination with AAV ECs. Among top drugs increasing AAV-mediated transduction, found vatalanib, an inhibitor multiple tyrosine kinase...
Abstract Funding Acknowledgements Type of funding sources: Foundation. Main source(s): IEO-Monzino Foundation Background Cardiac metastasis is considered a relatively rare malignancy, although an increasing prevalence expected in the future due to life expectancy. Several cases cardiac metastases remain untreatable, given anatomical location that often hampers both surgical eradication and bioptic collection for biological characterization. Here, we use spatial transcriptomics provide...
Abstract Funding Acknowledgements None. Background The adult heart is unable to regenerate after ischemic injury, primarily due the incapacity of cardiomyocyte (CM) re-enter in cell cycle and endothelial cells (EC) form new vessels. Interestingly, our recent data have unveiled that decline CM proliferation birth paralleled by a reduction angiogenic potential. Nevertheless, precise mechanisms governing angiogenesis inhibition remain elusive. We hypothesize terminal differentiation CMs leads...
Abstract Funding Acknowledgements None. Introduction The adult mammalian heart has a poor regenerative and angiogenic potential. At the same time, both primary cardiac tumors metastases are extremely rare. This suggests that shared mechanisms may halt proliferation of (both cardiomyocyte endothelial) cancer cells. Among have been claimed to be responsible for loss proliferative potential cardiomyocytes early after birth is sudden increase in mechanical load. Purpose Here, we investigate...
Abstract Background. New drugs to tackle the next pathway or mutation fueling cancer are constantly proposed, but 97% of them doomed fail in clinical trials, largely because they identified by cellular silico screens that cannot predict their vivo effect. Methods. We screened an Adeno-Associated Vector secretome library (> 1000 clones) directly a mouse model and validated therapeutic effect first hit, EMID2, both orthotopic genetic models lung pancreatic cancer. Results. EMID2...
Abstract Funding Acknowledgements Type of funding sources: None. Introduction Despite major attempts aiming to revascularize an ischemic heart, prognosis cardiac ischemia remains poor. A obstacle for the induction new blood vessels, referred as therapeutic angiogenesis, is inefficient gene transfer in endothelial cells (ECs). This poor permissiveness genetic modification a limiting step studies pathological conditions with inadequate, but also increased vascularization. To study function...
Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – EU funding. Main source(s): PREFER Introduction With the increased prevalence chronic diseases, non-healing wounds place a significant burden on health system, with 2-5%, similar to one heart failure. They are persistent full-thickness skin lesions that affect patients suffering from vascular disorders, such as diabetes and peripheral artery disease. Skin implants substitutes currently applied promote closure...
Abstract With the increased prevalence of chronic diseases, non-healing wounds place a significant burden on both quality life affected patients and health systems. Skin substitutes are applied to promote closure wounds, although their efficacy is limited by inadequate vascularization. The stromal vascular fraction (SVF) from adipose tissue has emerged as promising therapy overcome this limitation. Despite few successful clinical trials, its incorporation in routine been hampered...