A5025512287- Bacterial biofilms and quorum sensing
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Health, Nursing, Elderly Care
- Plant Pathogenic Bacteria Studies
- Social and Political Issues
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Social and Economic Solidarity
- Genomics and Phylogenetic Studies
- Legume Nitrogen Fixing Symbiosis
- Microbial Fuel Cells and Bioremediation
- Plant tissue culture and regeneration
- Nematode management and characterization studies
- Plant Pathogens and Fungal Diseases
- Biochemical and Structural Characterization
- Occupational Health and Burnout
- Youth, Drugs, and Violence
- Banana Cultivation and Research
- Microbial Community Ecology and Physiology
- Microbial Inactivation Methods
- Public Health in Brazil
- Plant Gene Expression Analysis
- Education Pedagogy and Practices
- Oral microbiology and periodontitis research
International Centre for Genetic Engineering and Biotechnology
2013-2024
International Centre for Genetic Engineering and Biotechnology
2024
Istituto Ramazzini
2020
Universidade Estadual Paulista (Unesp)
2004-2015
Instituto de Historia
2009-2010
National Center for Genetic Engineering and Biotechnology
2008
Universidade de Franca
2004-2007
Arkansas Agricultural Experiment Station
2007
Universidade de São Paulo
1995
Endophytes are harmless or beneficial microorganisms that live inside plants between cells. The relationship they develop with the plant as well their potential role in health is at large unexplored and it believed opportunity to find new interesting endophytes among variety of great. Here, we present isolation analysis a collection from one cultivar rice grown Italy. A total 1318 putative were isolated roots, leaves stems submerged dry conditions working 229 isolates was created. Among...
Abstract The use of microbial inoculants in agriculture as biofertilisers and/or biopesticides is an appealing alternative to replace or reduce the practice agrochemicals. Plant microbiota studies are revealing different bacterial groups which populating plant microbiomes re‐energising probiotic bacteria (PPB) translational research sector. Some single‐microbial strain bioinoculants have proven valid (e.g., based on Trichoderma , mycorrhiza rhizobia); however, it now recommended consider...
Summary The quorum sensing (QS) signalling system of Pseudomonas aeruginosa controls many important functions, including virulence. Although the production QS signal molecule N‐ 3‐oxo‐dodecanoyl‐homoserine lactone (3OC 12 ‐HSL) is positively autoregulated, its concentration reaches a steady level long before stationary phase. RsaL protein represses transcription lasI synthase gene, and thus reduces production. We show that binds simultaneously with LasR to rsaL‐lasI bidirectional promoter...
Resolution of bacterial infections is often hampered by both resistance to conventional antibiotic therapy and hiding cells inside biofilms, warranting the development innovative therapeutic strategies. Here, we report efficacy blue laser light in eradicating Pseudomonas aeruginosa cells, grown planktonic state, agar plates mature vitro vivo, with minimal toxicity mammalian tissues. Results obtained using knock-out mutants point oxidative stress as a relevant mechanism which exerts its...
ABSTRACT A mutation in the rsaL gene of Pseudomonas aeruginosa produces dramatically higher amounts N -acyl homoserine lactone with respect to wild type, highlighting key role this negative regulator controlling quorum sensing (QS) opportunistic pathogen. The DNA binding site RsaL protein on rsaL-lasI bidirectional promoter partially overlaps LasR protein, consistent hypothesis that and could be competition promoter. This is first direct demonstration acts as a QS by lasI
Pseudomonas aeruginosa possesses three quorum-sensing (QS) systems which are key in the expression of a large number genes, including many virulence factors. Most studies QS P. have been performed clinical isolates and therefore focused on its role pathogenicity. aeruginosa, however, is regarded as ubiquitous organism capable colonizing different environments also establishing beneficial associations with plants. In this study we examined two N-acyl homoserine lactone known RhlI/R LasI/R...
Burkholderia cenocepacia are opportunistic Gram-negative bacteria that can cause chronic pulmonary infections in patients with cystic fibrosis. These demonstrate a high-level of intrinsic antibiotic resistance to most clinically useful antibiotics complicating treatment. We previously identified 14 genes encoding putative Resistance-Nodulation-Cell Division (RND) efflux pumps the genome B. J2315, but contribution these drug this bacterium remains unclear. To investigate we deleted 3 operons...
Quorum sensing is a cell population-density dependent regulatory system which in gram-negative bacteria often involves the production and detection of N-acyl homoserine lactones (AHLs). Some Pseudomonas putida strains have been reported to produce AHLs, one quorum-sensing locus has identified. However, it appears that majority do not AHLs. In this study we report identification regulation AHL-dependent rhizosphere P. WCS358. This identical recently identified strain IsoF very similar las...
Refletir sobre o campo saúde do(a) trabalhador(a) é objetivo deste estudo. Busca-se sublinhar significado das condições de trabalho para ser humano do ponto vista da saúde. A premissa não quantificar, mas inferir que as podem gerar danos à saúde, nem sempre apresentam imediato a sua relação com trabalho. São discutidas, partir abordagem qualitativa, três situações, quais contemplam os trabalhos rural, informal e infantil e, como resultado, verifica-se contradição categoria trabalho, que, se...
Summary Pseudomonas fuscovaginae is a Gram‐negative fluorescent pseudomonad pathogenic towards several plant species. Despite its importance as pathogen, no molecular studies of virulence have thus far been reported. In this study we show that P. possesses two conserved N ‐acyl homoserine lactone (AHL) quorum sensing (QS) systems which designated PfsI/R and PfvI/R. The system homologous to the BviI/R Burkholderia vietnamiensis produces responds C10‐HSL C12‐HSL whereas PfvI/R LasI/R...
Background Members of swarming bacterial consortia compete for nutrients but also use a co-operation mechanism called quorum sensing (QS) that relies on chemical signals as well other secreted products ("public goods") necessary swarming. Deleting various genes this machinery leads to cheater mutants impaired in aspects cooperation. Methodology/Principal Findings Pairwise made Pseudomonas aeruginosa, its QS B. cepacia cells show interspecies consortium can "combine the skills" participants...
In Chromobacteium violaceum, the purple pigment violacein is under positive regulation by N-acylhomoserine lactone CviI/R quorum sensing system and negative an uncharacterized putative repressor. this study we report that biosynthesis of negatively controlled a novel repressor protein, VioS. The operon regulated VioS positively in both C. violaceum heterologous Escherichia coli genetic background. does not regulate apart from violacein, VioS, other phenotypes antagonistically. Quorum are...
Endophytes live inside plants and are often beneficial. Kosakonia is a novel bacterial genus that includes many diazotrophic plant-associated isolates. Plant-bacteria studies on two rice endophytic beneficial strains were performed, including comparative genomics, secretome profiling, in planta tests, field release trial. The efficient rhizoplane root endosphere colonizers localized the cortex. Secretomics revealed 144 putative secreted proteins, type VI secretory system (T6SS) proteins. A...
ABSTRACT Bacterial strains belonging to Burkholderia cepacia can be human opportunistic pathogens, plant and growth promoting have remarkable catabolic activity. B. consists of several genomovars comprising what is now known as the complex. Here we report quorum-sensing system a genomovar I onion rot type strain ATCC 25416. Quorum sensing cell-density-dependent regulatory response which involves production N -acyl homoserine lactone (HSL) signal molecules. The cep locus has been inactivated...
Flooding events caused by severe rains and poor soil drainage can interfere with plant germination seedling establishment. Rice is one of the cereal crops that has unique strategies under flooding. One these based on fast coleoptile elongation in order to reach water surface re-establish contact air. Microorganisms contribute health via growth promoters provide protection from abiotic stresses. To characterise community composition microbiome rice submergence, a 16S rRNA gene profiling...
Studies on bacterial plant diseases have thus far been focused the single species causing disease, with very little attention given to many other microorganisms present in microbiome. This study intends use pathobiome analysis of rice foot rot caused by Dickeya zeae, as a case investigate effects this pathogen total resident microbiome and highlight possible interactions between members community involved disease process. The asymptomatic foot-rot symptomatic field-grown plants over two...
In the opportunistic human pathogen Pseudomonas aeruginosa, quorum sensing (QS) is crucial for virulence. The RsaL protein directly represses transcription of lasI, synthase gene main QS signal molecule. On basis sequence homology, cannot be predicted to belong any class characterized DNA-binding proteins. this study, an in silico model structure was inferred showing that belongs tetrahelical superclass helix-turn-helix overall very similar N-terminal domain lambda cI repressor and...
Pseudomonas corrugata is a phytopathogenic bacterium, causal agent of tomato pith necrosis, yet it an ubiquitous bacterium that part the microbial community in soil and rhizosphere different plant species. Although very heterogeneous species, all strains tested were able to produce short chain acyl homoserine lactone (AHL) quorum sensing signal molecules. The main AHL produced was N-hexanoyl-L-homoserine (C(6)-AHL). An system, designated PcoI/PcoR, identified characterized. role system...
Quorum sensing (QS) is a form of gene regulation based on cell-density that depends inter-cellular communication. While there are variety models for bacterial colony morphology, little work linking QS genes to movement in an open system.The onset swarming environmental P. aeruginosa PUPa3 was described with simplified computational model which cells random motion communicate via diffusible signal (representing N-acyl homoserine lactones, AHL) as well diffusible, secreted factors (enzymes,...
Kosakonia oryzae KO348 is an endophytic and plant growth-promoting strain isolated from the roots of rice in Italy. Here, we report draft genome sequence KO348.