A5068671412- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Endometriosis Research and Treatment
- Complement system in diseases
- DNA Repair Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Blood groups and transfusion
- Ubiquitin and proteasome pathways
- COVID-19 Impact on Reproduction
- CRISPR and Genetic Engineering
- Neural dynamics and brain function
- Bioinformatics and Genomic Networks
- Antimicrobial Peptides and Activities
- Blood Coagulation and Thrombosis Mechanisms
- Aquaculture disease management and microbiota
- Histone Deacetylase Inhibitors Research
- Wnt/β-catenin signaling in development and cancer
- Ferroptosis and cancer prognosis
IRCCS Materno Infantile Burlo Garofolo
2023-2025
University of Trieste
2009-2022
Washington University in St. Louis
2021
The University of Melbourne
2013
Centro di Riferimento Oncologico
2013
European Molecular Biology Laboratory
2013
Scuola Internazionale Superiore di Studi Avanzati
2009
Breast cancer is a heterogeneous disease that progresses to the critical hallmark of metastasis. In present study, we show High Mobility Group A1 (HMGA1) protein plays fundamental role in this process basal-like breast subtype. HMGA1 knockdown induces mesenchymal epithelial transition and dramatically decreases stemness self-renewal. Notably, depletion cell lines reduced migration invasion vitro formation metastases vivo. Mechanistically, activated key migration-associated genes which were...
Breast cancer is the most common malignancy in women worldwide. Among breast subtypes, triple-negative (TNBC) aggressive and difficult to treat. One of master regulators TNBC progression architectural transcription factor HMGA1. This study aimed further explore HMGA1 molecular network identify mechanisms involved progression.RNA from MDA-MB-231 cell line, silenced for expression, was sequenced and, with a bioinformatic analysis, partners could cooperate regulating downstream gene networks...
// Silvia Pegoraro 1,* , Gloria Ros Yari Ciani 1,2 Riccardo Sgarra 1 Silvano Piazza 2 and Guidalberto Manfioletti Dipartimento di Scienze della Vita, Università degli Studi Trieste, Italy Laboratorio Nazionale CIB (LNCIB), Area Science Park, * These authors have contributed equally to this work Correspondence to: Manfioletti, email: Piazza, Keywords : cyclin E2, YAP/TAZ, hippo pathway, CDK inhibitors, oncogene/tumour suppressor Received October 20, 2014 Accepted May 12, 2015 Published 22,...
Endometriosis (EM) is a chronic inflammatory disorder with multifactorial etiologies (i.e., genetics and environmental factors, hormonal immunological changes, microbiome alterations). The complement system one of the most frequently dysregulated pathways in EM. Mannose-binding lectin (MBL), carbohydrate pattern recognition molecule, first described subcomponent pathway (LP). Here, we unveiled interplay among MBL polymorphisms, plasma levels, LP functionality, microbiota as potential...
Cancer is a very heterogeneous disease, and biological variability adds further level of complexity, thus limiting the ability to identify new genes involved in cancer development. Oncogenes whose expression levels control cell aggressiveness are useful for developing cellular models that permit differential screenings isogenic contexts. HMGA1 protein has this unique property because it master regulator breast cells transition from nontumorigenic epithelial-like phenotype toward highly...
Pregnancy is an immunologically regulated, complex process. A tightly controlled complement system plays a crucial role in the successful establishment of pregnancy and parturition. Complement inhibitors at feto-maternal interface are likely to prevent inappropriate activation protect fetus. In present study, we aimed understand Factor H (FH), negative regulator activation, normal model pathological pregnancy, i.e. preeclampsia (PE). The distribution expression FH was investigated placental...
Cancer cells secrete proteins that modify the extracellular environment acting as autocrine and paracrine stimulatory factors have a relevant role in cancer progression. The HMGA1 oncofetal protein has prominent controlling expression of an articulated set genes involved various aspect cell transformation. However, little is known about its influencing secretome cells. Performing iTRAQ LC-MS/MS screening for identification secreted proteins, inducible model silencing breast cells, we found...
Plasticity is an essential condition for cancer cells to invade surrounding tissues. The nucleus the most rigid cellular organelle and it undergoes substantial deformations get through environmental constrictions. Nuclear stiffness mostly depends on nuclear lamina chromatin, which in turn might be affected by architectural proteins. Among these HMGA1 (High Mobility Group A1) protein, a factor that plays causal role neoplastic transformation able disentangle heterochromatic domains H1...
Background Natural antisense long non-coding RNAs (lncRNAs) are regulatory transcribed from the opposite strand of either protein coding or genes, able to modulate their own sense gene expression. Hence, dysregulation can lead pathologic processes. Cancer is a complex class diseases determined by aberrant expression variety factors, among them, oncofetal chromatin architectural proteins High Mobility Group A (HMGA) several cancer hallmarks. Thus, we decided investigate presence natural...
Endometriosis (EM) is defined as the engraftment and proliferation of functional endometrial-like tissue outside uterine cavity, leading to a chronic inflammatory condition. While precise etiology EM remains elusive, recent studies have highlighted crucial involvement dysregulated immune system. The complement system one predominantly altered pathways in EM. Owing its process angiogenesis, here, we examined possible role first recognition molecule classical pathway, C1q. C1q plays seminal...
Abstract High Mobility Group A are non-histone nuclear proteins that regulate chromatin plasticity and accessibility, playing an important role both in physiology pathology. Their activity is controlled by transcriptional, post-transcriptional post-translational mechanisms. In this study we provide evidence for a novel modulatory mechanism HMGA functions. We show HMGAs complexed vivo with the histone chaperone nucleophosmin (NPM1), interaction requires histone-binding domain of NPM1...
Among breast cancer subtypes, triple-negative (TNBC) is the most aggressive with worst prognosis and highest rates of metastatic disease. To identify TNBC gene signatures, we applied network-based methodology implemented by SWIM software to expression data patients in The Cancer Genome Atlas (TCGA) database. enables predict key (switch) genes within co-expression network, whose perturbations pattern abundance may contribute (patho)biological phenotype. Here, analysis revealed an interesting...
Abstract Periods of intense electrical activity can initiate neuronal plasticity leading to long lasting changes network properties. By combining multielectrode extracellular recordings with DNA microarrays, we have investigated in rat hippocampal cultures the temporal sequence events triggered by a transient exposure GABA A receptor antagonist gabazine (GabT). GabT induced synchronous bursting pattern activity. The analysis identified three main phases during GabT: (i) immediately after...
The HMGA1 architectural transcription factor is an oncogene overexpressed in the vast majority of human cancers. a highly connected node nuclear molecular network and key aspect involvement cancer development that simultaneously confers cells multiple oncogenic hits, ranging from global chromatin structural gene expression modifications up to direct functional alterations cellular proteins. Interestingly, also modulates DNA damage repair pathways. In this work, we provide evidences linking...
Chromatin accessibility plays a critical factor in regulating gene expression cancer cells. Several factors, including the High Mobility Group A (HMGA) family members, are known to participate directly chromatin relaxation and transcriptional activation. The HMGA1 oncogene encodes an architectural transcription that alters DNA structure interacts with factors favouring their landing onto regulatory sequences. Here, we provide evidence of additional mechanism exploited by modulate...
Abstract Blockage of GABA‐A receptors in hippocampal neuronal cultures triggers synchronous bursts spikes initiating plasticity, partly mediated by changes gene expression. By using specific pharmacological blockers, we have investigated which sources Ca 2+ entry primarily control expression induced 20 µM gabazine applied for 30 min (GabT). Intracellular transients were monitored with imaging while recording electrical activity patch clamp microelectrodes. Concomitant transcription profiles...