A5023667036- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Cell Adhesion Molecules Research
- Force Microscopy Techniques and Applications
- Molecular Junctions and Nanostructures
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Nanofabrication and Lithography Techniques
- Biosensors and Analytical Detection
- T-cell and B-cell Immunology
- Forest Insect Ecology and Management
- Biochemical and Structural Characterization
- Iron Metabolism and Disorders
- Chemical Synthesis and Analysis
- HER2/EGFR in Cancer Research
- Caveolin-1 and cellular processes
- CAR-T cell therapy research
- Genomics and Chromatin Dynamics
- Neurological diseases and metabolism
- Viral gastroenteritis research and epidemiology
- Trace Elements in Health
Italian Institute of Technology
2024-2025
Karolinska Institutet
2018-2024
Elettra-Sincrotrone Trieste S.C.p.A.
2016-2019
AREA Science Park
2016-2017
National Interuniversity Consortium of Materials Science and Technology
2016-2017
University of Trieste
2016-2017
Vitenparken
2016
University of Insubria
2007
Abstract The precise molecular mechanism of how misfolded α-synuclein (α-Syn) accumulates and spreads in synucleinopathies is still unknown. Here, we show the role cellular prion protein (PrP C ) mediating uptake spread recombinant α-Syn amyloids. vitro data revealed that presence PrP fosters higher amyloid fibrils, which was also confirmed vivo wild type ( Prnp +/+ compared to knock-out −/− mice. Additionally, amyloids blocked replication scrapie prions Sc ex , indicating a link between two...
Programmed Death-1 (PD-1) is a coinhibitory receptor expressed on activated T cells that suppresses T-cell signaling and effector functions. It has been previously shown binding to its ligand PD-L1 induces spatial reorganization of PD-1 receptors into microclusters the cell membrane. However, roles organization clustering have not elucidated. Here, we used DNA origami flat sheets display ligands at defined nanoscale distances investigated their ability inhibit activation in vitro. We found...
Abstract Insulin binds the insulin receptor (IR) and regulates anabolic processes in target tissues. Impaired IR signalling is associated with multiple diseases, including diabetes, cancer neurodegenerative disorders. IRs have been reported to form nanoclusters at cell membrane several types, even absence of binding. Here we exploit nanoscale spatial organization achieve controlled multivalent activation. To control valency, developed rod-like insulin–DNA origami nanostructures carrying...
Plasticity is an essential condition for cancer cells to invade surrounding tissues. The nucleus the most rigid cellular organelle and it undergoes substantial deformations get through environmental constrictions. Nuclear stiffness mostly depends on nuclear lamina chromatin, which in turn might be affected by architectural proteins. Among these HMGA1 (High Mobility Group A1) protein, a factor that plays causal role neoplastic transformation able disentangle heterochromatic domains H1...
The oriented immobilization of proteins, key for the development novel responsive biomaterials, relies on availability effective probes. These are generally provided by standard approaches based in vivo maturation and vitro selection antibodies and/or aptamers. techniques can suffer technical problems when a non-immunogenic epitope needs to be targeted. Here we propose strategy circumvent this issue silico design. In our method molecular binders, form cyclic peptides, computationally evolved...
Early detection of cancer plays a crucial role in disease prognosis. It requires the recognition and quantification low amounts specific molecular biomarkers, either free or transported inside nanovesicles, through development novel sensitive diagnostic technologies. In this context, we have developed nanoarray platform for noninvasive biomarkers circulating bloodstream. The assay is based on manipulation to create functional spots surface-immobilized binders differential topography...
The deposition of biomolecules on biosensing surface platforms plays a key role in achieving the required sensitivity and selectivity for biomolecular interactions analysis. Controlling interaction between is increasingly becoming crucial design tool to modulate properties needed improve performance assay detection outcome. Carboxymethyl-dextran (CMD) coating can be exploited promote chemical grafting proteins, providing hydrophilic, bioinert, nonfouling high density immobilized proteins. In...
Background: DNA hybridization is at the basis of most current technologies for genotyping and sequencing, due to unique properties base-pairing that guarantee a high grade selectivity. Nonetheless presence single base mismatches or not perfectly matched sequences can affect response devices major challenge is, nowadays, distinguish mismatch and, same time, unequivocally differentiate read-out fully partially matching sequences. Results: We present here two platforms based on different...
The availability of devices for cancer biomarker detection at early stages the disease is one most critical issues in biomedicine. Towards this goal, to increase assay sensitivity, device miniaturization strategies empowered by employment high affinity protein binders constitute a valuable approach. In work we propose two different surface-based miniaturized platforms body fluids: first platform an atomic force microscopy (AFM)-based nanoarray, where AFM used generate functional nanoscale...
ABSTRACT Autoimmune diseases, driven by Fcγ receptor (FcγR) activation through autoantibody immune complexes (IC), present a complex therapeutic challenge of achieving pharmacological blockade FcγR without triggering activation. The assembly ICs into polydisperse, higher-order structures is required for However, engineered multimeric, monodisperse Fc assemblies have been reported to prevent activation, suggesting that spatial organization determines In this study, we functional single-chain...
Transferrin receptor 1 (TfR) delivers iron across cellular membranes by shuttling the ion carrier protein transferrin. This ability to deliver large ligands inside cells is taken advantage of pathogens infiltrate human cells. Notably, receptor's outermost ectodomain, apical domain, used as a point attachment for several viruses including hemorrhagic arenaviruses. To better understand interactions with it would be advantageous probe sequence determinants in domain viral spike proteins. Here,...
Human transferrin receptor 1 (TfR) is necessary for the delivery of iron carrier protein into cells and can be utilized targeted across cellular membranes. Binding to regulated by hereditary hemochromatosis (HFE), an regulatory that partly shares a binding site with on TfR. Here, we derived essential interactions from HFE computationally grafted these library small scaffolds. One designed proteins, TB08, was further optimized experimentally identify variants improved The variant, TB08 S3.1,...