A5043117515- Platelet Disorders and Treatments
- Exercise and Physiological Responses
- Complement system in diseases
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Iron Metabolism and Disorders
- Blood groups and transfusion
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Pain Mechanisms and Treatments
- Trace Elements in Health
- Blood properties and coagulation
- Receptor Mechanisms and Signaling
- Muscle metabolism and nutrition
- Plant Micronutrient Interactions and Effects
- Protein Structure and Dynamics
- Hepatitis B Virus Studies
- Blood transfusion and management
- Blood donation and transfusion practices
- RNA Interference and Gene Delivery
- Periodontal Regeneration and Treatments
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Blood Coagulation and Thrombosis Mechanisms
- Viral Infections and Vectors
Linnaeus University
2020-2025
Linköping University
2014-2015
Abstract Chronic widespread pain (CWP) has a high prevalence in the population and is associated with prominent negative individual societal consequences. There no clear consensus concerning etiology behind CWP although alterations central processing of nociception maintained by peripheral nociceptive input been suggested. Here, we use proteomics to study protein changes trapezius muscle from 18 female patients diagnosed compared 19 healthy subjects. The 2-dimensional gel electrophoresis...
Introduction The functionalization of nanoparticles (NPs) with an antiCD19 targeting mechanism represents a promising approach for the selective delivery drugs and nucleic acids into normal tumor B cells. This strategy has advantage minimizing off-target effects by restricting gene to desired cell population. However, nanoplatform must guarantee both local production protein safety treatment allow effective therapy reduced systemic toxicity. Methods In order ensure acids, we developed...
Complement analysis necessitates strict control of pre-analytical blood handling, including time, temperature, and additives. Here, we compared complement function activation status across five different serum preparations two plasma preparations. Serum was collected from ten healthy volunteers using glass tubes without additives, with a silica clot activator (with or gel separator), containing thrombin separator). Plasma in the presence EDTA inhibitor lepirudin. aliquots were snap-frozen...
Iron oxide nanoparticles (IONPs) are widely used in diagnostic and therapeutic settings. Upon systemic administration, however, they rapidly recognized by components of innate immunity, which limit their capacity can potentially lead to adverse side effects. IONPs were previously found induce the inflammatory response human whole blood, including activation complement system increased secretion cytokines. Here, we investigated thromboinflammatory 10-30 nm lepirudin anticoagulated blood...
Platelet activation and the complement system are mutually dependent. Here, we investigated effects of storage time on platelet function in routinely produced concentrates. The concentrates (n = 10) were stored at 22 °C for seven days assessed daily markers. Additionally, was analyzed terms their responsiveness to protease-activated receptor-1 (PAR-1) thromboxane A2 receptor (TXA2R) capacity adhere collagen. Complement increased over period all markers, including C1rs/C1-INH complex (fold...
Factor I (FI) is an essential regulator of the complement system. Together with co-factors, FI degrades C3b, which inhibits further activation. Genetic mutations in are associated pathological conditions like age-related macular degeneration and atypical hemolytic uremic syndome. Here, we evaluated eight recombinant genetic variants found patients. We assessed FI's co-factor activity presence two co-factors; H soluble CR1. Different analytical assays were employed; SDS-PAGE to evaluate...
Supply of iron into human cells is achieved by carrier protein transferrin and its receptor that upon complex formation get internalized endocytosis. Similarly, the needs to be delivered brain, necessitates transport across blood-brain barrier. While there are still unanswered questions about these mechanisms, extensive efforts have been made use system for delivery therapeutics biological compartments. The dimeric form receptor, where each subunit consists three domains, further complicates...
Machupo virus, known to cause hemorrhagic fevers, enters human cells via binding with its envelope glycoprotein transferrin receptor 1 (TfR). Similarly, the interactions have been explored in biotechnological applications as a molecular system ferry therapeutics across cellular membranes and through impenetrable blood-brain barrier that effectively blocks any such delivery into brain. Study of experimental structure virus (MGP1) complex TfR sequence homology has identified some residues at...
Transferrin receptor 1 (TfR) delivers iron across cellular membranes by shuttling the ion carrier protein transferrin. This ability to deliver large ligands inside cells is taken advantage of pathogens infiltrate human cells. Notably, receptor's outermost ectodomain, apical domain, used as a point attachment for several viruses including hemorrhagic arenaviruses. To better understand interactions with it would be advantageous probe sequence determinants in domain viral spike proteins. Here,...
Human transferrin receptor 1 (TfR) is necessary for the delivery of iron carrier protein into cells and can be utilized targeted across cellular membranes. Binding to regulated by hereditary hemochromatosis (HFE), an regulatory that partly shares a binding site with on TfR. Here, we derived essential interactions from HFE computationally grafted these library small scaffolds. One designed proteins, TB08, was further optimized experimentally identify variants improved The variant, TB08 S3.1,...