A5068744447- Celiac Disease Research and Management
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Blood properties and coagulation
- Microscopic Colitis
- Autoimmune Bullous Skin Diseases
- Galectins and Cancer Biology
- Protein purification and stability
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Rheumatoid Arthritis Research and Therapies
- Transgenic Plants and Applications
- Eosinophilic Esophagitis
- T-cell and B-cell Immunology
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Cell Adhesion Molecules Research
- Systemic Lupus Erythematosus Research
- HER2/EGFR in Cancer Research
- Digestive system and related health
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Research and Splicing
- Helicobacter pylori-related gastroenterology studies
University of Trieste
2015-2025
University of Maribor
2020
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2007-2016
PromoFirenze
2015
Department of Medical Sciences
2015
Piedmont University
2008-2010
Los Alamos National Laboratory
2003-2010
IRCCS Istituto Auxologico Italiano
2005
University of Milan
2005
Istituti di Ricovero e Cura a Carattere Scientifico
2005
OBJECTIVE: Tissue transglutaminase is the autoantigen recognized by sera of celiac patients. An enzyme-linked immunosorbent assay (ELISA) based on guinea-pig tissue was recently used to measure serum antibodies for diagnosis disease. We determine sensitivity and specificity an ELISA test use human recombinant transglutaminase, compared with guinea pig IgA antiendomysium antibodies. METHODS: Serum samples were tested from 65 patients intestinal biopsy proven disease, 10 Crohn's 150 healthy...
To determine coeliac disease prevalence by an anti-transglutaminase antibody assay in a large paediatric population; to evaluate acceptance of the screening programme, dietary compliance, and long term health effects.Cross-sectional survey 3188 schoolchildren (aged 6-12) prospective follow up diagnosed cases. Main outcome measures were: defined intestinal biopsy or positivity both human tissue transglutaminase anti-endomysium antibodies HLA DQ2-8 positive subjects; percentage children whose...
Celiac disease (CD) is an intestinal malabsorption characterized by intolerance to cereal proteins accompanied immunological responses dietary gliadins and tissue transglutaminase, autoantigen located in the endomysium. Tissue transglutaminase belongs family of enzymes that catalyze protein cross-linking reactions constitutively expressed many tissues as well being activated during apoptosis. The role eliciting immune response CD how linked primary reaction are still unclear. In this work,...
Tissue transglutaminase is the autoantigen recognized by sera of celiac patients. An enzyme-linked immunosorbent assay (ELISA) based on guinea-pig tissue was recently used to measure serum antibodies for diagnosis disease. We determine sensitivity and specificity an ELISA test use human recombinant transglutaminase, compared with guinea pig IgA antiendomysium antibodies.Serum samples were tested from 65 patients intestinal biopsy proven disease, 10 Crohn's 150 healthy blood donors.Human...
Background Celiac disease (CD) is an autoimmune gastrointestinal disorder characterized by the presence of anti-transglutaminase 2 (TG2) and anti-gliadin antibodies. Amongst neurological dysfunctions associated with CD, ataxia represents most common one. Methods We analyzed immunohistochemistry, anti-neural reactivity serum from 20 CD patients. To determine role anti-TG2 antibodies in ataxia, two single chain variable fragments (scFv), isolated a phage-display IgA antibody library, were...
We have developed a high-throughput protein expression and interaction analysis platform that combines cDNA phage display library selection massive gene sequencing using the 454 platform. A of open reading frame (ORF) fragments was created from mRNA derived different tissues. This used to study network enzyme transglutaminase 2 (TG2), multifunctional involved in regulation cell growth, differentiation apoptosis, associated with many pathologies. After two rounds panning TG2 we assayed...
Despite recent efforts in discovering novel long non-coding RNAs (lncRNAs) and unveiling their functions a wide range of biological processes applications as biotechnological or therapeutic tools are still at infancy. We have recently shown that AS Uchl1, natural lncRNA antisense to the Parkinson's disease-associated gene Ubiquitin carboxyl-terminal esterase L1 (Uchl1), is able increase UchL1 protein synthesis post-transcriptional level. Its activity requires two RNA elements: an embedded...
<h3>Introduction</h3> Despite climate change being recognised as the greatest health threat of 21st century, current medical education curricula do not reflect urgency crisis. Preparing for climate-related repercussions requires educational institutions to disseminate planetary knowledge in a systematic way. We sought evaluate extent literature on inclusion undergraduate and postgraduate guide curricular development. <h3>Methods analysis</h3> A scoping review is undertaken with search...
Expression of the complement-regulatory proteins (CRP) CD46, CD55 and CD59 represents a strategy used by tumor cells to evade complement-dependent cell cytoxicity stimulated monoclonal antibodies.We have isolated two single-chain variable fragments (scFv) from human phage-display library these scFv we produced miniantibodies (MB), MB-55 (against CD55) MB-59 CD59), containing hinge-CH2-CH3 domains IgG1.The specificity MB for corresponding CRP was assessed ELISA using purified CD59.MB-55...
It is generally accepted that plasmids containing the same origin of replication are incompatible. We have re-examined this concept in terms plasmid copy number, by introducing and different antibiotic resistance genes into bacteria. By selecting for to only one antibiotic, we were able examine persistence carrying resistances other antibiotics. find not rapidly lost, but persist bacteria multiple overnight growth cycles, with some dependence upon nature selected for. out experiments origins...
The multifunctional, protein cross-linking transglutaminase 2 (TG2) is the main autoantigen in celiac disease, an autoimmune disorder with defined etiology. Glutamine-rich gliadin peptides from ingested cereals, after their deamidation by TG2, induce T-lymphocyte activation accompanied autoantibody production against TG2 1–2% of population. pathogenic role and exact binding properties these antibodies to are still unclear. Here we show that different patients target same conformational...
Drug delivery strategies using chitosan nanobubbles (CS-NBs) could be used to reduce drug side effects and improve outcomes in hepatocellular carcinoma (HCC) treatment. To enhance their action, a targeting agent, such as the humanized anti-GPC3 antibody GC33 (condrituzumab), attached surface. Here, we investigated use of idarubicin-loaded CS-NBs for HCC treatment GC33-derived minibody (that named 4A1) CS-NB delivery. Various formulations were prepared with or without 4A1 conjugation...
Introduction The functionalization of nanoparticles (NPs) with an antiCD19 targeting mechanism represents a promising approach for the selective delivery drugs and nucleic acids into normal tumor B cells. This strategy has advantage minimizing off-target effects by restricting gene to desired cell population. However, nanoplatform must guarantee both local production protein safety treatment allow effective therapy reduced systemic toxicity. Methods In order ensure acids, we developed...
Coeliac disease is an autoimmune that primarily associated with chronic inflammation of the gut, but can also affect organs outside from liver to skin and CNS. The triggered in predisposed individuals by a peptide mixture (PT) derived digestion gliadin, component wheat, which ingested food. Although induction endoplasmic reticulum stress intestinal epithelial cells (IECs) upon exposure PT known, underlying molecular mechanisms remain unclear. Identifying key players this signaling pathway...
<h3>Background and objective</h3> Antitransglutaminase (anti-TG2) antibodies are synthesised in the intestine their presence seems predictive of future coeliac disease (CD). This study investigates whether mucosal represent an early stage gluten intolerance even absence intestinal damage serum anti-TG2 antibodies. <h3>Methods</h3> investigated 22 relatives patients with CD genetically predisposed to but negative for both abnormalities. Fifteen subjects were symptomatic seven asymptomatic....
Abstract Osteoblasts, osteocytes, and osteoclasts (OCs) are involved in the bone production resorption, which crucial homeostasis. OC hyperactivation plays a role exaggerated resorption of diseases such as osteoporosis, rheumatoid arthritis, osteolytic tumor metastases. This work stems from finding that OCs can express B7h (ICOS-Ligand), is ligand ICOS T cell costimulatory molecule. Because recent reports have shown that, endothelial, dendritic, cells, triggering modulates several activities...